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Cellular and molecular mechanism involved in the newt retinal regeneration

Research Project

Project/Area Number 13680807
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Developmental biology
Research InstitutionNara Women's University

Principal Investigator

ARAKI Masasuke  Nara Women's University, Faculty of Science, Professor, 理学部, 教授 (00118449)

Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2002: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2001: ¥2,800,000 (Direct Cost: ¥2,800,000)
KeywordsRetina / Retinal Pigment Epithelium / Regeneration / Newt / FGF-2 / IGF-1 / Organ Culture / 脈絡膜 / IGF-1 / BrdU / 組織間相互作用
Research Abstract

Transdifferentiation from retinal pigment epithelium (RPE) to neural retina (NR) was studied under a new culture system as an experimental model for newt retinal regeneration. Adult newt RPEs were organ-cultured with the choroid on a filter membrane. RPE cells migrated out of the explant and became lightly pigmented "neuron-like cells" with neuritic processes, which were positively stained for neuron-specific antibodies. BrdU-labeling study showed that these cells proliferate under culture condition. When RPE sheets were isolated from the choroid and cultured under the same condition, RPE cells neither grow nor differentiate into neural cells, while RPE cells differentiated to neurons when the isolated RPE sheets were reattached to the choroid and cultured. In the presence of FGF-2, however, RPE cells of isolated RPE sheets began to proliferate and differentiate to neurons. IGF-1 had no effects on RPE cell growth but intensely promoted neural differentiation when added in combination with FGF-2. Isolated RPE cells also proliferated substantially when it was co-cultured with the choroid with a culture filter membrane inbetween these two tissues. These results indicated that the choroid plays an essential role in retinal rgeneration ot the newt and suggests that diffusible factors are involved in this tissue interaction. FGF-2 and IGF-1 are considered as the candidate molecules.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Ikegami Y, Mitsuda S, Araki M: "Neural cell differentiation from retinal pigment epithelial cells of the newt : An organ culture model for the urodele retinal regeneration"J.Neurobiol.. 50. 209-220 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Araki M, Takano T, et al.: "Epithelia-mesencyme interaction plays an essential role in transdifferentiation of retinal pigment epithelium of silver mutant quail"Develop.Biol.. 244. 358-371 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hata K, Araki M, Yamamori T: "Ciliary neurotrophic factor is specifically expressed in developing rat pineal gland and eyes"NeuroReport. 13. 735-739 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shimauchi Y, Yahata T, Matubara S, Araki M: "Role of tissue interaction between pineal primordium and neighboring tissues in avian pineal morphogenesis studied by intraocular transplantation"Dev.Gene Evol.. 212. 319-329 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Uemonsa T, Skagami K, Yasuda K, Araki M: "Development of dorsal-ventral polarity in the optic vesicle studied by embroynic transplantation and in ovo culture"Develop.Biol.. 248. 319-330 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Araki M, Nagata K, et al.: "Developmentally regulated expression of Neuro-p24 and its possible function in neutrite extension"Neurosci.Res.. 44. 379-389 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ikegami Y, Mitsuda S and Araki M: "Neural cell differentiation from retinal pigment epithelial cells of the newt : An organ culture model for the urodele retinal regeneration"J.Neurobiol. 50. 209-220 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Araki M, Takano T, et al.: "Epithelia-mesenchyme interaction plays an essential role in transdifferentiation of retinal pigment epithelium of silver mutant quail : Localization of FGF and related molecules and aberrant migration pattern of neural crest cells during eye rudiment formation"Devlop.Biol.. 244. 358-371 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hata K, Araki M and Yamamori T: "Ciliary neurotrophic factor is specifically expressed in developing rat pineal gland and eyes"NeuroReport. 13. 735-739 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shimauchi Y, Yahata T, Matubara S and Araki M: "Role of tissue interaction between pineal primordium and neighboring tissues in avian pineal morphogenesis studied by intraocular transplantation"Dev.Gene Evol.. 212. 319-329 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Uemonsa T, Sakagami K, Yasuda K and Araki M: "Development of dorsal-ventral polarity in the optic vesicle studied by embryonic transplantation and in ovo culture"Develop.Biol.. 248. 319-330 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Araki M, Nagata K, et al.: "Developmentally regulated expression of Neuro-p24 and its possible function in neurite extension"Neurosci.Res.. 44. 379-389 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hata K, Araki M and Yamamori T: "Ciliary neurotrophic factor inhibits differentiation of photoreceptor-like cells in rat pineal glands in vitro"Dev.Brain Res.. (in press). (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ikegami Y, Mitsuda S, Araki M: "Neural cell differentiation from retinal pigment epithelial cells of the newt : An organ culture model for the urodele retinal regeneration"Journal of Neurobiology. 50. 209-220 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Araki M et al.: "Epithelia-mesenchyme interaction plays an essential role in transdifferentiation of retinal pigment epithelium of silver mutant quail : Localization of FGF and related molecules and aberrant migration pattern of neural crest cells during eye rudiment formation"Developmental Biology. 244. 358-371 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Uemonsa T et al.: "Development of dorsal-ventral polarity in the optic vesicle studied by embryonic transplantation and in ovo culture"Developmental Biology. 248. 319-330 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Shimauchi Y et al.: "Role of tissue interaction between pineal primordium and neighboring tissues in avian pineal morphogenesis studied by intraocular transplantation"Development Gene and Evolution. 212. 319-329 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 荒木正介, 右衛門佐知子, 池上陽子: "網膜の発生と再生に結合組織(間葉組織)はいかなる役割をもつか"モレキュラーメディシン. 39. 38-46 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ikegami et al.: "Neural cell differentiation from netinal pigment epitheleal cells of the neut"Journal of Neurobiology. 50. 209-220 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] 荒木 正介: "網膜・視神経の発生と再生(分担)"中山書店. 266 (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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