The study for the function of synapsins in Synaptic vesicles and secretory granules

• Project/Area Number: 13680837
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: Gunma University
• Principal Investigator: HOSAKA Masahiro Department of Melecular Medicine, Institute for Molecular and Cellular Regulation, Gunma University, Assistant Professor, 生体調節研究所, 講師 (80311603)
• Project Period (FY): 2001 – 2002
• Project Status: Completed (Fiscal Year 2002)
• Budget Amount: ¥3,600,000 (Direct Cost: ¥3,600,000); Fiscal Year 2002: ¥1,700,000 (Direct Cost: ¥1,700,000); Fiscal Year 2001: ¥1,900,000 (Direct Cost: ¥1,900,000)
• Keywords: Synapsin / Synaptic Vesicles / Chromogranin A / Secretogranin III / ATPase / Secretory Granules

Research Abstract

A project of Synapsin in Synaptic vesicles
Synapsins constitute a family of synaptic vesicle proteins essential for regulating neurotransmitter release. Only two domains are conserved in all synapsins : a short N-terminal A domain with a single phosphorylation site for cAMP-dependent protein kinase (PKA) and CaM Kinase I, and a large central C domain that binds ATP and may be enzymatic. With this research grant, we tried to show that the C-domain of synapsins works as an ATPase and which unknown substrates co-laborate with synapsins. For these aims, we employed a yeast two-hybrid system with rat cDNA library. We are still analysing some candidate proteins.
A project of Sorting Mechanisms to Secretory Granule in Neuroendocrine Cells
Chromogranin A (CgA) is transported restrictedly to regulated secretory granules in neuroendocrine cells. Because the binding-prone property of CgA with secretory proteins may be essential for its sorting to secretory granules, we screened its binding partner proteins using a yeast two-hybrid system. As results, we identified that SgIII binds to CgA and directs CgA to secretory granules by specific interactions in various endocrine cells.

Research Products

• [Publications] Sakai Y, Hosaka M, Hira Y, Harumi T, Ohsawa Y, Wang H, Takeuchi T, Uchiyama Y, Watanabe T: "Immunocytochemical localization of secretogranin III in the anterior lobe of male rat pituitary glands"J Histochem Cytochem. 51. 227-238 (2003)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Hosaka M, Watanabe T, Sakai Y, Uchiyama Y, Takeuchi T: "Identification of a chromogranin A domain that mediates binding to secretogranin III and targeting to secretory granules in pituitary cells and pancreatic β-cells"Mol Biol Cell. 13. 3388-3399 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Sakai Y, Hosaka M. Hira Y, Harumi T, Ohsawa Y, Wang H, Takeuchi T, Uchiyama Y, Watanabe T.: "Immunocytochemical localization of secretogranin III in the anterior lobe of male rat pituitary glands"J Histochern Cytochem.. 51. 227-238 (2003)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Hosaka M. Watanabe T, Sakai Y, Uchiyama Y, Takeuchi T.: "Identification of a chromogranin A domain that mediates binding to secretogranin III and targeting to secretory granules in pituitary cells and pancreatic β-cells"Mol Biol Cell.. 13. 3388-3399 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Sakai Y, Hosaka M et al.: "Immunocytochemical localization of secretogranin III in the anterior lobe of male rat pituitary glands"J Histochem Cytochem. 51. 227-238 (2002)
• [Publications] Hosaka M et al.: "Identification of a chromogranin A domain that mediates binding to secretogranin III and targeting to secretory granules in pituitary cells and pancreatic beta-cells"Mol Biol Cell. 13. 3388-3399 (2002)
• [Publications] Yi Z, et al.: "The rab27a/granuphilin complex regulates the exocytosis of insulin-containing dense-core granules"Mol Cell Biol. 22. 1858-1867 (2002)