| Project/Area Number |
13680839
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | Shinshu University |
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Principal Investigator |
SUZUKI Tatsuo Shinshu University School of Medicine, Professor, 医学部, 教授 (80162965)
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| Co-Investigator(Kenkyū-buntansha) |
TIAN Qingbao Shinshu University School of Medicine, Research Associate, 医学部, 助手 (40324264)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2001: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | PSD / postsynaptic density / synaptic plasticity / local protein synthesis / poetsynaptic density / postsynaptic density / dendrite |
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| Research Abstract |
We cloned from a rat brain cDNA library more than ten novel full-length cDNAs, whose mRNAs are localized to postsynaptic sites (we call them Dem genes). We characterized some of the Dem proteins. 1
Results obtained are as follows.
1. synUSP : a novel ubiquitin-specific protease (USP), which is enriched in the PSD fraction and localized to dendrites in the rat brain. It possesses USP enzyme activity. We also identified an interacting protein by yeast two-hybrid method.
2. synArfGEF : a novel GEF for Art, which is enriched in the PSD fraction and localized to dendrites in the rat brain. Pull-down assay showed interaction with PSD-95 and SAP97 via a C-terminal PDZ-binding motif.
3. ssLRP : a novel LDL receptor-like protein which is localized to the postsynaptic sites and localized to dendrites in the rat brain. It interacted with PSD-95 and SAP97 via a C-terminal PDZ-binding motif. Ligands are still under investigation.
4. Another Dem protein, TANC and p55L appear to be scaffold proteins for PSD. They interacted with various other PSD proteins, including PDZ-containing proteins and cytoskeletal proteins.
5. We plan to produce knockout mice for synUSP and ssLRP genes to know their physiological functions and relation to some types of disease.
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