| Project/Area Number |
13680848
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
SANO Mamoru Kyoto Prefectural University of Medicine, Professor, 医学研究科, 教授 (60090429)
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| Project Period (FY) |
2001 – 2003
|
| Project Status |
Completed (Fiscal Year 2003)
|
| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2003: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2002: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2001: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | radicicol / geldanamycin / Hsp90 / NF108-15 / N2a / DRG / ラディシコール / ゲルダナマイシン / ニューロトロフィン / 知覚神経節 |
|---|
| Research Abstract |
Radicicol and geldanamycin, Hsp9O-binding antibiotics, potentiate neurite growth and survival of cultured sensory neurons from chick embryo. They did not exhibit trophic effects on the sensory neurons from embryonic and adult rats and mice. We show that the antibiotics induced the outgrowth of neurites in NG108-15 cells and N2A cells. Treatment of cells with these drugs decreased levels of Raf1,ERK1/2 and phosphorylated ERK1/2 time-dependently. Levels of Akt1 and CDK4 were slightly decreased. The neurite growth by the drugs was blocked by actynomycin D. The neurite growth of NG108-15 cells Geld might be due to a change in the synthesis of some specific protein(s).
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