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Developmental mechanisms from tetal to neonatal type of respiratory center as a functional neuron network

Research Project

Project/Area Number 13680880
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Neuroscience in general
Research InstitutionShowa University

Principal Investigator

ONIMARU Hiroshi  Showa Univ School of Med., Associate Professor, 医学部, 助教授 (30177258)

Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 2002: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2001: ¥1,900,000 (Direct Cost: ¥1,900,000)
Keywordsrespiratory center / development / fetus / prainnstem-spiralcord / respiratory neuron / optical recording / neonate / rat / 顔面神経核 / バースト形成 / ホールセル記録
Research Abstract

We analyzed intrinsic burst generating property of respiratory neurons in the medulla of the rat fetus. In the E17-20 preparations that showed the fetal type-C4 inspiratory burst pattern, about 60% of inspiratory neurons exhibited voltage-dependent burst activity. In E19 preparations, the input resistance of the bursting neurons was significantly higher than that of non-bursting cells. Membrane capacitance tended to be lower (but not significant) in the bursting neurons than in the non-bursting cells. Since the bursting neurons still kept the bursting activity in the low Ca2+/high Mg2+ solution, we concluded that they possess intrinsic burst generating properties.
Using optical recordings, we analyzed respiratory neuron network activity in the ventral medulla of fetus stained with voltage-sensitive dye. In E20/21 preparations, respiratory neuron activity appeared in the limited area of the rostral ventrolateral medulla (para-facial respiratory group, pFRG), and propagated mainly caudall … More y. Then, during the inspiratory phase, the peak of activity was detected, caudally, in the caudal ventrolateral medulla (CVLM) and, rostrally, in the mainly lateral and medial region of the facial nucleus. Such pattern was basically identical to that of neonatal rat preparations. In contrast, in all of El7 and most of El8 preparations, respiratory activity first appeared in the CVLM region, coincidently with the start of inspiratory burst, and then propagated rostrally. Successively, strong activity appeared in the whole region of the facial nucleus and continued 1-3 sec after termination of C4 inspiratory activity. In half of E19 preparations, respiratory activity started from the pFRG (neonatal type), whereas it started from the CVLM in
others (fetal type). However, even in the preparations that showed the neonatal type-network activity pattern, C4 inspiratory activity was the fetal type showing short burst duration. We suggest that the primary rhythm generating network and the spatio-temporal pattern develops to the neonatal type before the neuron network generating inspiratory activity does. Less

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] Onimaru, H.: "A Novel Functional Neuron Group for Respiratory Rhythm Generation in the Ventral Medulla"The Journal of Neuroscience. 23. 1478-1486 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Onimaru, H.: "Development of the rat respiratory neuron network during the late fetal period"Neuroscience Research. 42. 209-218 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Janczewski, W.A.: "Opioid-resistant respiratory pathway from the preinspiratory neurons to abdominal muscles : in vivo and in vitro study in the newborn rat"Journal of Physiology. 545. 1017-1026 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tokumasu, M.: "Optical recording of spontaneous respiratory neuron activity in the rat brainstem"Jpn. J. Physiol.. 51. 613-619 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takeda, S.: "Opioid Action on Respiratory Neuron Activity of the Isolated Respiratory Network in Newborn Rats"Anesthesiology. 95. 740-749 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Onimaru, H. and Homma, I.: "A novel functional neuron group for respiratory rhythm generation in the ventrolateral medulla"J. Neurosci.. 23. 1478-1486 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Janczewski,W.A. ,Onimaru,H. and Homma, I.: "Opioid-resistant respiratory pathway from the preinspiratory neurones to abdominal muscles - in vivo and in vitro study in the newborn rat"J. Physiol.. 545. 1017-1026 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Onimaru, H. and Homma, I.: "Development of the rat respiratory neuron network during the late fetal period"Neuroscience Research. 42. 209-218 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Matsuura, T. , Kumagai, H. , Kawai, A. , Onimaru, H., Imai, M., Oshima, N., Sakata, K. and Saruta, T.: "Different responses of rostral ventrolateral medulla neurons to Angiotensin II and Candesartan in neonatal Wistar-Kyoto and spontaneously hypertensive rats"Hypertension. 40. 560-565 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takeda, S. , Eriksson,L. I. , Yamamoto,Y. , Joensen,H. , Onimaru,H. and Lindahl,S. G. E.: "Opioid action on respiratory neuron activity of the isolated respiratory network in newborn rats"Anesthesiology. 95. 740-749 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tokumasu, M. , Nakazono, Y., Ide, H., Akagawa, K., and Onimaru, H.: "Optical recording of spontaneous respiratory neuron activity in the rat brainstem"Jpn J Physiol. 51. 613-619 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Onimaru, H.: "A Novel Functional Neuron Group for Respiratory Rhythm Generation in the Ventral Medulla"The Journal of Neuroscience. 23. 1478-1486 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Onimaru, H.: "Development of the rat respiratory neuron network during the late fetal period"Neuroscience Research. 42. 209-218 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Janczewski, W.A.: "Opioid-resistant respiratory pathway from the preinspiratory neurons to abdominal muscles : in vivo and in vitro study in the newborn rat"Journal of Physiology. 545. 1017-1026 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Onimaru, H.: "Development of the rat respiratory neuron network during the late fetal period"Neuroscience Research. (in press).

    • Related Report
      2001 Annual Research Report
  • [Publications] Tokumasu, M.: "Optical recording of spontaneous respiratory neuron activity in the rat brainstew"Jpn J Physiol.. 51. 613-619 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Takeda, S.: "Opioid Action on Respiratory Neuron Activity of The Isolated Respiratory Network in Newborn Rats"Anesthesiology. 95. 740-749 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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