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Real-time analysis on activity-dependent glucose uptake by usiug brain slices

Research Project

Project/Area Number 13680893
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 神経・脳内生理学
Research InstitutionAkita University

Principal Investigator

YAMADA Katsuya  Akita University School of Medicine Research Associate, 医学部, 助手 (40241666)

Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2002: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2001: ¥2,300,000 (Direct Cost: ¥2,300,000)
Keywordsglucose / fluorescence / uptake / brain / gloucose / flourescent / tracer
Research Abstract

Energy supply in the brain solely depends on glucose. However, the relationship between brain activity and glucose supply has not been well understood. By using 2-NBDG as a fluorescent glucose tracer, we have for the first time visualized glucose uptake into a single, living mammalian cells (Yamada et al, J. Biol. Chem. 2000). Our aim in the present study was to visualize glucose uptake into neurons. In conclusion, neurons dissociated from ventromedial hypothalamus, substantia nigra pars reticulata, and visual cortex incorporated 2-NBDG and showed an increase in the fluorescence. 2-NBDG was applied to visual cortical slices while the underlying white matter was electrically stimulated. Changes in the fluorescence intensity were detected in the area above the stimulating electrodes. In vivo experiments were also conducted in the somatosensory barrel cortex while the whiskers were mechanically stimulated after injection of 2-NBDG into artery. 2-NBDG fluorescence was observed in the site where optical intrinsic signals were the highest, implying an activity-dependent glucose uptake although more detailed study should be conducted.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] Yamada, K.: "Protective role of ATP-sensitive potassium channels in hypoxia-induced generalize seizure"Science. 292. 1543-1546 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yamada, K.: "Metabolic control of the seizure gate: a role for neuronal K-ATP channels"Trends in Pharmacological Sciences. 22. 601-602 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yamada, K.: "ATP-sensitive potassium channels in the brain : a sensor of hypoxic conditions"News in Physiological Sciences. 17. 127-130 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 山田勝也: "エネルギー欠乏時に脳を守るATP感受性カリウムチャネルの働き"メディカルテクノロジー. 30. 165-166 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 山田勝也: "単一膵島細胞におけるグルコース取り込みと細胞内カルシウムの観察 分子糖尿病学の進歩-基礎から臨床まで-2001.監修 矢崎義雄 渥美他編"金原出版. 187 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yamada,K., Ji,J.-J., Yuan,H., Miki,T., Sato,S., Horimoto,N., Shimizui,T., Seino,S., Inagaki,N.: "Protective role of ATP-sensitive potassium channels in hypoxia-induced generalized seizure."Science. 292. 1543-1546 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yamada,K., Inagaki,N: "Metabolic control of the seizure gate: a role for neuronal K-ATP channels."Trends Pharmacol. Sci.. 22. 601-602 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yamada, K., Inagaki,N.: "ATP-sensitive potassium channels in the Brain: a sensor of Iryposic conditions."News Physiol. Sci.. 17. 127-130 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yamada, K., Inagaki, N.: "A role of ATP-sensitive potassium channels: a protection for the brain during energy-depleting state"Medical Technology. 30. 165-166 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yamada, K., Nakata, M., Inagaki, N.: "Measurement of glucose uptake and intracellular calcium levels in a single pancreatic Islet cells in Bunshitonyobyogaku no sinpo -kisokararinnshoumade-"Yazaki Y., Ed. Kanehara Shuppan. 187 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yamada, K.: "Protective role of ATP-sensitive potassium channels in hypoxia-induced generalize seizure"Science. 292・5521. 1543-1546 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yamada, K.: "Metabolic control of the seizure gate : a role for neuronal K-ATP channels"Trends in Pharmacological Sciences. 22・12. 601-602 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yamada, K.: "ATP-sensitive potassium channels in the brain : a sensor of hypoxic conditions"News in Physiological Sciences. 17. 127-130 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 山田勝也: "エネルギー欠乏時に脳を守るATP感受性カリウムチャネルの働き"メディカルテクノロジー. 30・2. 165-166 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 山田勝也: "単一膵島細胞におけるグルコース取り込みと細胞内カルシウムの観察 分子糖尿病学の進歩-基礎から臨床まで-2001 監修 矢崎義雄 渥美他編"金原出版. 187 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yamada, K., et al.: "Protective role of ATP-sensitive potassium channels in hypoxia-induced generalized seizure"Science. 292. 1543-1546 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yamada, K., et al.: "Metabolic control of the seizure gate : a role for neuronal K-ATP channels"Trends in Pharmacological Sciences. 22. 601-602 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 山田勝也, 他: "エネルギー欠乏時に脳を守るATP感受性カリウムチャネルの働き"メディカルテクノロジー. 30・2. 165-166 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] 山田勝也, 他: "単一膵島細細胞におけるグルコース取り込みと細胞内カルシウムの観察 分子糖尿病学の進歩-基礎から臨床まで-2001"金原出版. 187 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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