| Project/Area Number |
13832005
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Institution | Yamaguchi University |
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Principal Investigator |
MIZUKAMI Yoichi Yamaguchi University Center for Gene Research, Associate Professor, 遺伝子実験施設, 助教授 (80274158)
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| Co-Investigator(Kenkyū-buntansha) |
KOBAYASHI Sei Yamaguchi University, School of Medicine, Professor, 医学部, 教授 (80225515)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2002: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2001: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | heart / ischemia / signal transduction / seretion / protein ourification / mass spectrometry / kinase / charomatography / 分泌 |
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| Research Abstract |
We purify survival factors released from cadiomyocytes exposed to ischemia. The supernatant of 100 liter was collected from cultured cardiomyocytes, and concentrated by ammonium sulfate. The sample was separated by column chromatography, and tested survival activity for cardiomyocytes. Two proteins having survival activity were identified using mass spectrometry. The molecular masses of these proteins are 33 kDa and 41 kDa, respectively. These proteins induce serine phosphorylation of 30 kDa protein, and the purified proteins show survival activity. We speculate that these proteins play a role for understanding of ischemia in heart and for development of drug discovery.
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