骨転移研究のためのマイクロ流体環流システムを用いた３次元骨髄微小環境の構築
| Project/Area Number |
13F03049
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| Research Category |
Grant-in-Aid for JSPS Fellows
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| Allocation Type | Single-year Grants |
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| Section | 外国 |
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| Research Field |
Microdevices/Nanodevices
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| Research Institution | The University of Tokyo |
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Principal Investigator |
竹内 昌治 東京大学, 生産技術研究所, 教授 (90343110)
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| Co-Investigator(Kenkyū-buntansha) |
HSIAO Amy 東京大学, 生産技術研究所, 外国人特別研究員
HSIAO AMY Yu-ching 東京大学, 生産技術研究所, 外国人特別研究員
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2014: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2013: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | 3D cell culture / Tissue engineering / Cancer cell / Microfluidic / Mesenchymal stem cells / Cell fiber / 3D cell Culture / tissue engineering / microfluidics |
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| Outline of Annual Research Achievements |
The main purpose of this research is to engineer the bone marrow niche microenvironment in vitro for metastatic cancer studies using micro-technologies. In the original plan, we proposed to include cancer cells, osteoblasts, and endothelial cells in the system. During the process, we learned about the importance of mesenchymal stem cells and pursued to create three-dimensional (3D) microenvironments from mesenchymal stem cells (MSCs). Initially, multipotent dedifferentiated fat (DFAT) cells that possess similar differentiation abilities as MSCs were used. DFAT cells were encapsulated in the core region of alginate shell fibers using a microfluidic double co-axial device to make 3D cell fiber constructs and differentiated into the smooth muscle cell lineage. Subsequently MSCs derived from adipose tissue, adipose-derived stem cells (ADSCs), were cultured as cell fiber constructs using the same cell fiber technology and differentiated into adipocytes. The conditioned media from the differentiated ADSC fibers was further used to culture MCF-7 breast cancer cells to study its effect on breast cancer cell proliferation.
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| Research Progress Status |
26年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
26年度が最終年度であるため、記入しない。
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Report
(2 results)
Research Products
(4 results)
