ＣＡＧＥ、 ５＇ シーケンス・タグテクノロジーを使ってのガンにおける代わりのプロ
| Project/Area Number |
13F03706
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| Research Category |
Grant-in-Aid for JSPS Fellows
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| Allocation Type | Single-year Grants |
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| Section | 外国 |
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| Research Field |
ゲノム生物学
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| Research Institution | 0182401 |
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Principal Investigator |
フォレスト アリスター 独立行政法人理化学研究所, ライフサイエンス技術基盤研究センター, チームリーダー
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| Co-Investigator(Kenkyū-buntansha) |
カチコフスキー ボグミル 独立行政法人理化学研究所, ライフサイエンス技術基盤研究センター, 外国人特別研究員
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| Project Period (FY) |
2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | Mesothelioma / Therapeutic target / Pan—cancer / Biomarker / protein coding genes / long non—coding RNAs / Differential Expression / Drug sensitivity |
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| Research Abstract |
We have used the CAGE data (genome-wide, 5' sequence tag technology) from FANTOM5 project to identify promoter that are aberrantly used in mesothelioma cancers. We compared the expression data from 16 mesothelioma lines to that of the normal primary mesothelial cells from 3 donors. Using differential gene expression we identified a, set of genes that are specifically up-regulated in the mesothelioma lines. Amongst these was the Oxytocin Receptor (OXTR).
To confirm that the up-regulation we studied a microarray dataset of 39 primary mesotheliomas, 64 other cancers and 9 normal lung/pleura samples. 50% of the mesothelioma tumor samples expressed OXTR at levels above that in the normal lung tissue. To test whether suppressing the OXTR can kill mesothelioma cells, we examined the sensitivity of 6 mesothelioma cell lines to Atosiban, which is a clinically approved OXTR antagonist. We find that 2 of the mesothelioma cell lines with high OXTR expression were sensitive to Atosiban. When working with FANTOM5 data and comparing mesothelioma cell lines to normal mesothelial cells, we noticed what some promoters show a general up- or down-regulation in cancer. We extended the analysis to 12 cancer origins comparing cancer cell lines to their counterpart normal primary cells. In a comprehensive analysis we identified a list of universal, pan cancer promoters (of both protein coding genes and long non-coding RNA), which is meant for the wet lab validation studies.
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| Strategy for Future Research Activity |
I don't fill out this section, because FY2013 is the final project year.
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Report
(1 results)
Research Products
(2 results)
