生命1次情報の高速収集とそれに基づく統合バイオサイエンスの展開

• Project/Area Number: 13GS0016
• Research Category: Grant-in-Aid for Creative Scientific Research
• Allocation Type: Single-year Grants
• Research Institution: National Institutes of Natural Sciences Okazaki Research Facilities (2004-2005); Okazaki National Research Institutes (2001-2003)
• Principal Investigator: 永山 國昭 (永山 国昭) 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), 岡崎統合バイオサイエンスセンター, 教授 (70011731)
• Co-Investigator(Kenkyū-buntansha): 森 泰生 京都大学, 大学院工学研究科, 教授 (80212265) ; 岡村 康司 (岡村 靖司) 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), 岡崎統合バイオサイエンスセンター, 教授 (80201987) ; 宇理須 恒雄 (宇理須 恆雄) 分子科学研究所, 極端紫外光科学研究系, 教授 (50249950) ; 青野 重利 岡崎国立共同研究機構, 統合バイオサイエンスセンター, 教授 (60183729) ; 高橋 卓也 岡崎国立共同研究機構, 計算科学研究センター, 助手 (70262102) ; 渡辺 芳人 岡崎国立共同研究機構, 統合バイオサイエンスセンター, 教授 (10201245)
• Project Period (FY): 2001 – 2005
• Project Status: Completed (Fiscal Year 2005)
• Budget Amount: ¥403,000,000 (Direct Cost: ¥335,500,000、Indirect Cost: ¥67,500,000); Fiscal Year 2005: ¥91,000,000 (Direct Cost: ¥70,000,000、Indirect Cost: ¥21,000,000); Fiscal Year 2004: ¥91,000,000 (Direct Cost: ¥70,000,000、Indirect Cost: ¥21,000,000); Fiscal Year 2003: ¥110,500,000 (Direct Cost: ¥85,000,000、Indirect Cost: ¥25,500,000); Fiscal Year 2002: ¥110,500,000 (Direct Cost: ¥110,500,000)
• Keywords: 電子顕微鏡 / 位相回復法 / DNAシーケンサー / チャネル / 蛋白質 / カルシウム / 電位センサー / バイオセンサー / 電位依存性蛋白質 / DNA / シーケンサー / 人口酵素 / 信号制御 / 自己組織 / 人工酵素 / 自己組織化

Research Abstract

[複素顕微鏡]炭素膜を用いる位相板には物質透過に伴う電子線損失がある。この問題を解決し像の感度を上げるため無損失位相板の開発を試みた。Aharnov-Bohm効果を用いると、ベクトルポテンシャルが電子線の位相を変えるため電子線損失がない。ループ型微小磁石と棒型微小磁石の2つの位相板につきテストし、棒磁石型の場合無損失位相板が成功した。
[チャネル蛋白質]形質膜における、一酸化窒素(NO)センサーカチオンチャネルとして働くTRPC5による、NO感知の分子機構を明らかにした。TRPC5のチャネル腔を形成するpore領域近傍のシステイン残基を、NOはニトロシル化し、その結果生じるコンフォメーション変化により、空間的に近接する内部ゲートが開くことが示された。
[電位センサー蛋白質]イノシトールリン脂質のうちPIP2によって活性が変化することが知られているKチャネルを電位センサー分子(VSP)とともにアフリカツメガエル卵母細胞へ強制発現させ計測し、酵素活性が膜電位依存的に制御されることを見出した。更に電位センサードメインをもちボア領域を欠く別の膜タンパクがチャネル活性をもつことを示した。
ヒト電位依存性NaチャネルNav1.6分子の機能の多様性を明らかにするためアンキリンGとNav1.6を共発現させ不活性化に及ぼす影響を検討した。
[蛋白質機能素子作製]シリコン基板に微細貫通孔を形成する技術を開発し、ここに脂質二重膜/イオンチャンネル(グラミシジン)を再構成して単一イオンチャンネル電流を計測することに成功した。微細孔構造を工夫することで、シリコン基板として世界最小の雑音電流(貫通孔径50μmで〜1pA rms、テフロン基板と同程度)を得ることが出来た。

Research Products

• [Journal Article] Carboxysomes in Living Cyanobacterial Cell Visualized by Hilbert Differential Contrast Transmission Electron Microscopy (2006)
  • Author(s): Y.Kaneko, R.Danev, K.Nagayama, H.Nakamoto
  • Journal Title: J.Bacterol 188 Pages: 805-808
• [Journal Article] Self-assembly of nano-sized arrays on highly oriented thin films of poly (2006)
  • Author(s): M.Tosaka, M.Tsuji, T.Ogawa, H.Kitano, K.Nakano, S.Kohjiya, R.Danev, K.Nagayama
  • Journal Title: Polymer 47 Pages: 951-955
• [Journal Article] Ca^<2+>/calmodulin dependent myosin light chain kinase is essential for activation of TRPC5 channels expressed in HEK cells. (2006)
  • Author(s): Shimizu S, Yoshida T, Wakamori M, Ishii M, Okada T, Takahashi M, Seto M, Sakurada K, Kiuchi Y, Mori Y
  • Journal Title: J. Physiol. 570 Pages: 219-235
• [Journal Article] Phoneutria nigriventer _<(1)>-Phonetoxin IIA : A new tool for anti-calcium channel autoantibody assays in Lambert-Eaton myasthenic syndrome. (2006)
  • Author(s): Martin-Moutot N, Haro LD, Santos RG, Mori Y, Seagar M
  • Journal Title: Neurobiol. Dis. (in presss)
• [Journal Article] Intracellular-produced hydroxyl radical mediates H202-induced TRPM2 channel activation and cell death in rat β-cell line RIN-5F. (2006)
  • Author(s): Ishii M, Shimizu S, Hara Y, Hagiwara T, Miyazaki A, Mori Y, Kiuchi Y
  • Journal Title: Cell Calcium (in presss)
• [Journal Article] A functional AMPA receptor-calcium channel complex in the postsynaptic membrane. (2006)
  • Author(s): Kang, MG, Chen CC, Wakamori M, Hara Y, Mori Y, Campbell KP
  • Journal Title: Proc. Natl. Acad. Sec. USA (in presss)
• [Journal Article] A critical role of TRPM2 in neuronal cell death by hydrogen peroxide (2006)
  • Author(s): Kaneko S, Kawakami S, Hara Y, Itoh E, Minami T, Takada Y, Kume T, Katsuki H, Mori Y, Akaike A
  • Journal Title: J Pharmacol. Sci (in presss)
  • NAID: 10018237407
• [Journal Article] A novel protein with a voltage sensor domain is a voltage-gated proton channel (2006)
  • Author(s): M.Sasaki, M.takagi, Y.OKAMURA
  • Journal Title: Science(on line published) March 23
• [Journal Article] 膜電位を感じて活性を変化させるイノシトールリン脂質脱リン酸化酵素 (2006)
  • Author(s): 岡村康司, 村田喜理, 岩崎広英, 佐々木真理
  • Journal Title: タンパク質 核酸 酵素 51 Pages: 18-26
• [Journal Article] 電位センサーの動作原理と電位感受性酵素VSP (2006)
  • Author(s): 岡村康司
  • Journal Title: 細胞工学 25 Pages: 231-235
• [Journal Article] In vivo subcellular ultrastructures recognized with Hilbert-differential-contrast transmission electron microscopy (2005)
  • Author(s): Y.Kaneko, R.Danev, K.Nitta, K.Nagayama
  • Journal Title: J. Electr. Microsc 54 Pages: 79-85
  • NAID: 10015519980
• [Journal Article] Transfer doublet and an Elaborated Phase Plate Holder for 120kV Electro-Phase Microscope (2005)
  • Author(s): F.Hosokawa, R.Danev, Y.Arai, K.Nagayama
  • Journal Title: J. Electr. Microsc 54 Pages: 317-324
• [Journal Article] Depositions of lipid bilayers on OH-density -controlled silicon dioxide surfaces (2005)
  • Author(s): R.Tero, T.Urisu, H.Okawara, K.Nagayama
  • Journal Title: J. Vac. Sci. Technol A23 Pages: 751-754
• [Journal Article] Application of Phase Contrast Transmission Microscopic Methods to Polymer Materials (2005)
  • Author(s): M.Tosaka, R.Danev, K.Nagayama
  • Journal Title: Macromolesules 38 Pages: 7884-7886
• [Journal Article] FPL-64176 modification of Ca_v1.2 L-type calcium channels : dissociation of effects on ionic current and gating current. (2005)
  • Author(s): McDonough SI, Mori Y, Bean BP
  • Journal Title: Biophys. J. 81 Pages: 211-223
• [Journal Article] Comprehensive analysis of the ascidian genome reveals novel insights into the molecular evolution of ion channel genes (2005)
  • Author(s): Okamura Y, murata Y, Nakajo K, Iwasaki I, Ohtsuka Y, Tanaka-Kumisima M, Takahashi N, Hara Y, Yoshida T, Nisida M, Okado H, Watari H, Meinertzhagen IA, Nisnino A, Satoh N, Takahashi K, Satou Y, Okada Y, Mori Y
  • Journal Title: Physiological Genomics 222 Pages: 269-282
• [Journal Article] The non-selective cation permeating channel TRPC3 is a tetrahedron with a cap in the large cytoplasmic end (2005)
  • Author(s): Mio K, Ogura T, Hara Y, Mori Y, SatoC
  • Journal Title: Biochem. Biophys. Res. Comm. 333 Pages: 768-777
• [Journal Article] The role of canonical transient receptor potential 7 (TRPC7) in B-cell receptor-activated channels. (2005)
  • Author(s): Lievremont J-P, Numaga Y, Vazquez G, Lemonnier L, Hara Y, Mori E, Trebak M, Trebak M, Moss SE, Bird GS, Mori Y, Putney Jr JW
  • Journal Title: J. Biol. Chem. 280 Pages: 35346-35351
• [Journal Article] Phosphoinositide phosphatase activity coupled to an intrinsic voltage sensor (2005)
  • Author(s): Y.Murata, H.Iwasaki, M.Sasaki, K.Inaba, Y.Okamura
  • Journal Title: Nature 435 Pages: 1239-1243
• [Journal Article] GABAergic synaptic transmission modulates swimming in the ascidian larva (2005)
  • Author(s): E.R.Brown, A.Nishino, Q.Bone, I.A.Meinertzhagen, Y.Okamura
  • Journal Title: Eur. J. Neurosci 22 Pages: 2541-2548
• [Journal Article] Tight junctions in Schwann cells of peripheral myelinated axons : a lesson from claudin-19-deficient mice (2005)
  • Author(s): T.Miyamoto, K.Morita, D.Takemoto, K.Takeuchi, Y.Kitano, T.Miyakawa, K.Nakayama, Y.Okamura, H.Sasaki, Y.Miyachi, M.Furuse, S.Tsukita
  • Journal Title: J Cell Biol. 169 Pages: 527-538
• [Journal Article] イオンチャネル遺伝子から見た生理機能の進化 (2005)
  • Author(s): 岡村康司
  • Journal Title: 海洋 41 Pages: 61-70
• [Journal Article] イオンの出入りを介さず膜電位シグナルを伝えるタンパク質Ci-VSP (2005)
  • Author(s): 岩崎広英, 村田喜理, 佐々木真理, 岡村康司
  • Journal Title: 実験医学 23 Pages: 2942-2944
• [Journal Article] Formation of high resistance supported lipid bilayer on the surface of Si substrate with micro electrodes in printing (2005)
  • Author(s): T.Urisu, Md.M.Rahman, H.Uno, R.Tero, PhD, Y.Nonogaki
  • Journal Title: Nanomedicine 1 Pages: 317-322
• [Journal Article] Orientation of avidin molecules immobilized on COOH-modified Si0_2/Si(100) surfaces (2005)
  • Author(s): N.Misawa, S.Yamamura, R.Tero, Y.Nonogaki, T.Urisu
  • Journal Title: Chem. Phys. Lett 419 Pages: 86-90
• [Journal Article] Giant Vesicle Fusion on Microelectrods Fabricated by Femto Second Laser Ablation Followed by Synchrotron Radiation Etching (2005)
  • Author(s): Md.M.Rahman, Y.Nonogaki, R.Tero, Yong-Hoon Kim, H.Uno, Zheng-Long Zhang, T.Yano, M.Aoyama, R.Sasaki, H.Nagai, M.Yoshida, T.Urisu
  • Journal Title: Jpn. J. Appl. Phys 44
  • NAID: 130004533549
• [Journal Article] Supported lipid bilayer formation by the giant vesicle fusion induced by vesicle-surface electrostatic attractive interaction (2005)
  • Author(s): Yong-Hoon Kim, Md.M.Rahman, Zhen-Lon ero, T.Urisu
  • Journal Title: Chem. Phys. Lett (in press)
• [Journal Article] Size-Selective Olefin Hydrogenation by a Pd Nanocluster Provided in an Apo-Ferritin Cage. (2004)
  • Author(s): Ueno T, Suzuki M, Goto T, Matsumoo T, Nagayama K, Watanabe Y
  • Journal Title: J.Angew.Chem.Int.Ed. 43 Pages: 2527-2530
• [Journal Article] Complex Observation in Electron Microscopy. IV. Reconstruction of Complex Object Wave from Conventional and Half Plane Phas (2004)
  • Author(s): Danev R, Nagayama T
  • Journal Title: J.Phys.Soc.Jpn. 73 Pages: 2718-2724
• [Journal Article] Complex Observation in Electron Microscopy. V. Phase Retrieval for Strong Objects with Foucault Knife-edge Scanning. (2004)
  • Author(s): Nagayama K
  • Journal Title: J.Phys.Soc.Jpn. 73 Pages: 2725-2731
  • NAID: 110001979195
• [Journal Article] Novel real time sensors to quantitatively assess in vivo inositol 1,4,5-trisphosphate production in intact cells. (2004)
  • Author(s): Sugimoto K, Nishida M, Otsuka M, Ohkubo K, Mori Y, Morii T.
  • Journal Title: Chem.Biol. 11 Pages: 475-485
• [Journal Article] Ca^<2+> store-independent augmentation of [Ca^<2+>]_i responses to G-protein coupled receptor activation in recombinantly TRPC5-expressed rat pheochromocytoma (PC12) cells. (2004)
  • Author(s): Ohta T, Morishita M, Mori Y, Ito S.
  • Journal Title: Neurosci.Lett. 358 Pages: 161-164
• [Journal Article] Involvement of TRPM7 in cell growth as spontaneously activate Ca^<2+> entry pathway in human retinoblastoma cells. (2004)
  • Author(s): Hanano T, Hara Y, Shi J, Itonaga Y, Mori E, Ito Y, Mori Y, Inoue R.
  • Journal Title: J Pharmacol.Sci. 95 Pages: 403-419
  • NAID: 10014165738
• [Journal Article] Identification of R(-)-isomer of efonidipine as a selective blocker of T-type Ca^<2+> channels. (2004)
  • Author(s): Furukawa T, Miura R, Honda M, Kamiya N, Mori Y, Takeshita S, Isshiki T, Nukada T.
  • Journal Title: J.Pharmacol. 143 Pages: 1050-1057
• [Journal Article] Multiple regulation by calcium of murine homologues of transient receptor potential proteins TRPC6 and TRPC7 expressed in HEK293 cell. (2004)
  • Author(s): Shi J, Mori E, Mori Y, Mori M, Li J, Ito Y, Inoue R.
  • Journal Title: J.Physiol. 561 Pages: 415-432
• [Journal Article] Properties of the neural net that controls swimming in the ascidian C.intestinalis. (2004)
  • Author(s): Brown ER, Bone Q, Okamura Y
  • Journal Title: Comp Biochem Physiol Part A 137
• [Journal Article] Ankyin G negatively regulates persistent current of neuronal sodium channel, Nav1.6, in heterologous expressions. (2004)
  • Author(s): Okamura Y
  • Journal Title: Jap.J.Physiol. 54
• [Journal Article] Development of transient outward currents coupled with Ca2+-induced Ca2+ release mediates oscillatory membrane potential in ascidian muscle. (2004)
  • Author(s): Nakajo K, Okamura Y
  • Journal Title: J Neurophysiol 92(2) Pages: 1056-1066
• [Journal Article] Early specification of ascidian larval motor neurons. (2004)
  • Author(s): Katsuyama Y, Okada T, Matsumoto J, Ohtsuka Y, Terashima T, Okamura Y
  • Journal Title: Dev Biol 5;278 Pages: 310-322
• [Journal Article] Characterization of dipalmitoylphosphatidylcholine (DPPC)/cholesterol Langmuir-Blodgett monolayers by AFM and FT-IR (2004)
  • Author(s): Kim Y.-H., Takizawa M, Urisu T
  • Journal Title: Jpn.J.Appl.Phys 43 Pages: 3860-3864
• [Journal Article] Deposition of phospholipid layers on SiO_2 surface modified by hyidrophobic SAM islands (2004)
  • Author(s): Tero R, Takizawa M, Li Y.-J., Yamazaki M, Urisu T
  • Journal Title: Appl.Surf Sci. 238 Pages: 218-222
• [Journal Article] Deposition of 10-undecenoic acid self assembled layers on H-Si(111) surfaces studied with AFM and FT-IR (2004)
  • Author(s): Li Y.-J., Tero R, Nagasawa T, Nagata T, Urisu T
  • Journal Title: Appl.Surf Sci. 238 Pages: 238-241
• [Journal Article] Deposition of DPPC monolayers by Langmuir-Blodget method on SiO_2 surfaces covered by octadecyltrichlorosilane self-assembled monolayer islands (2004)
  • Author(s): Takizawa M, Kim Y.-H., Urisu T
  • Journal Title: Chem.Phys.Lett. 385 Pages: 220-224
• [Journal Article] Lipid membrane formation by vesicle fusion on silicon dioxide surfaces modified with alkyl self-assembled-monolayer -islands (2004)
  • Author(s): Tero R, Takizawa M, Li Y.-J., Yamazaki M, Urisu T
  • Journal Title: Langmuir 20 Pages: 7526-7531
• [Journal Article] Structure and deposition mechanism of 10-undecenoic acid self-assembled layers on H-Si (111) surfaces studied by AFM and FT-IR (2004)
  • Author(s): Li Y.-J., Tero R, Nagasawa T, Nagata T, Haruyama Y, Urisu T
  • Journal Title: Jpn.J.Appl.Phys. 43 Pages: 4591-4594
• [Journal Article] Tight Junctions in Schwann cells of peripheral myelinated axons : a lesson from claudin-19-deficient mice
  • Author(s): Miyamoto T, Morita K, Takemoto D, Takeuchi K, Kitano Y, Miyakawa T, Nakayama K, Okamura Y, Sasaki H, Miyachi Y, Furuse M, Tsukita S
  • Journal Title: J Cell Biol (in press)
• [Journal Article] Regulation of Na_v channels in sensory neurons.
  • Author(s): Chahine M, Vijayaragavan K, Oksnura Y
  • Journal Title: Trends in Pharmacological Sciences (in press)
• [Journal Article] Activity of a phosphoinositide phosphatase is coupled to its intrinsic voltage-dependence accepted.
  • Author(s): Murata Y, Iwasaki H, Sasaki M, OKAMURA Y
  • Journal Title: Nature (accepted)
• [Book] Ad. Imaging Electr. Phys., vol.138, "Phase Contrast Enhancement with Phase Plates in Electron Microscopy" (2005)
  • Author(s): Kuniaki Nagayama
  • Publisher: Academic Press
• [Book] イオンチャネルの発現制御機構(標準生理学、第6版) (2005)
  • Author(s): 岡村康司
  • Publisher: 医学書院
• [Book] Ca channel pharmacology book (2004)
  • Author(s): Mori Y, Itsukaichi Y, Nishida M, Oka H.
• [Book] 新訂版・表面科学の基礎と応用 (2004)
  • Author(s): 宇理須恒雄
  • Total Pages: 932
  • Publisher: (株)エネ・テイー・エス
• [Patent(Industrial Property Rights)] 電子顕微鏡用位相及びその製造方法 (2005)
  • Inventor(s): 氷山國昭
  • Industrial Property Rights Holder: 自然科学研究機構Nagayama IP Holdings, LLC
  • Filing Date: 2005-11-04
• [Patent(Industrial Property Rights)] 位相差電子顕微鏡装置 (2005)
  • Inventor(s): 永山國昭
  • Industrial Property Rights Holder: 自然科学研究機構Nagayama IP Holdings, LLC
  • Filing Date: 2005-11-15
• [Patent(Industrial Property Rights)] Method of Determining Base Sequence of DNA or RNA and DNA Sequencer. (2005)
  • Inventor(s): 永山國昭
  • Industrial Property Rights Holder: Nagayama IP Holdings, LLC
  • Filing Date: 2005-05-16
• [Patent(Industrial Property Rights)] 佐藤矩行, 岡村康司, 岩崎広英, 村田喜理 (2005)
  • Inventor(s): 岡村康司, 佐藤矩行, 岩崎広英, 村田喜理
  • Industrial Property Rights Holder: 国立大学法人京都大学, 自然科学研究機構, 佐藤矩行, 岡村康司, 岩崎広英, 村田喜理
  • Filing Date: 2005-05-13
• [Patent(Industrial Property Rights)] 電位活性化がたプロトンチャネルを構成するタンパク質及びその利用法 (2005)
  • Inventor(s): 岡村康司, 佐々木真理
  • Industrial Property Rights Holder: 自然科学研究機構, 岡村康司, 佐々木真理
  • Filing Date: 2005-09-05
• [Patent(Industrial Property Rights)] タンパク質チップ、タンパク質チップ解析装置、及びタンパク質チップ解析方法 (2005)
  • Inventor(s): 宇理須恒雄, 鈴井光一
  • Industrial Property Rights Holder: 化学技術振興財団
  • Filing Date: 2005-04-21
• [Patent(Industrial Property Rights)] 立相差電子顕微鏡用位相板及びその製造方法並びに位相差電子顕微鏡 (2004)
  • Inventor(s): Radostin Danev, 永山 國昭
  • Industrial Property Rights Holder: 自然科学研究機構
  • Filing Date: 2004-12-03
• [Patent(Industrial Property Rights)] タンパク質チップ、タンパク質チップ解析装置、及びタンパク質チップ解析方法 (2004)
  • Inventor(s): 宇理須 恒雄, 鈴井 光一
  • Industrial Property Rights Holder: 独立行政法人科学技術振興機構
  • Industrial Property Number: 2004-133877
  • Filing Date: 2004-04-28
• [Patent(Industrial Property Rights)] 新規イオンチャネル様ポリペプチドおよびその利用
  • Inventor(s): 岡村 康司
  • Industrial Property Number: 2004-332070
• [Publications] M.Kato, S.hokabe, S.Itakura, S.Minoshima, Y.L.Lyubchenko, T.D.Gurkov, H.Okawara, K.Nagayama, N.Shimizu: "Interarm Interaction of DNA Cruciform Forming at a Short Inverted Repeat Sequence"Biophys.J.. 85. 402-408 (2003)
• [Publications] F.Hosokawa, R.Danev, Y.Arai, K.Nagayama: "Transfer Doublet and an Elaborated Phase Plate Holder for 120kV Electron-Phase Microscope"J.Electr.Microsc.. (In press). (2004)
• [Publications] K.Nagayama, R.Danev: "Image Enhancement with Phase Plates in Electron-Phase Microsocpy"Microscopy and Analysis. 34. 13-16 (2003)
• [Publications] 永山國昭: "解剖学の復権-低温電顕トモグラフィー"蛋白質・核酸・酵素. 48. 257-258 (2003)
• [Publications] 永山國昭: "電子位相顕微鏡の原理と実践"電子顕微鏡. 382. 29-35 (2003)
• [Publications] H.Sawai, N.Kawada, K.Yoshizato, H.Nakajima, S.Aono, Y.Shiro: "Characterization of the heme environmental structure of cytoglobin, a fourth globin in humans."Biochemistry. 42・17. 5133-5142 (2003)
• [Publications] S.Aono: "Biochemical and biophysicalproperties of the CO-sensing transcriptional activator CooA."Acc.Chem.Res.. 36・11. 825-831 (2003)
• [Publications] S.Aono, H.Nakajima, T.Ohta, T.Kitagawa: "Resonance Raman and ligand binding analysis of the oxygen-sensing signal transducer protein HemAT from Bacillus subtilis"Methods in Enzymology. (印刷中). (2004)
• [Publications] 青野重利: "一酸化炭素センサーとして機能する転写調節因子CooAの構造と機能"Molecular Medicine. 40・2. 160-164 (2003)
• [Publications] 青野重利: "一酸化炭素による遺伝子発現制御"バイオサイエンスとバイオインダストリー・span. A61・2. 37-38 (2003)
• [Publications] Erxleben C, Allegria-Gomez C, Darden, Mori Y, Birnbaumer L, Armstrong DL: "Modulation of cardiac Ca_v 2.1 channels by BAYk8644 and Okadaic acid requires S1142 in the domain III pore loop."Proc.Natl.Acad.Sci.USA. 100. 2929-2934 (2003)
• [Publications] Shimizu S, Shiota K, Yamamoto S, Miyasaka Y, Ishii M, Watabe T, Nishida M, Mori Y, Yamamoto T, Kiuchi Y: "Hydrogen peroxide stimulates tetrahydrobiopterin synthesis through the induction of GTP-cyclohydrolase I and increases nitric oxide synthase activity in vascular endothelial cells."Free Radic.Biol.Med.. 34. 1343-1352 (2003)
• [Publications] Sugimoto K, Mori Y, Makino K, Ohkubo K, Morii T: "Functional Reassembly of a Split PH Domain."J.Am.Chem.Soc.. 125. 5000-5004 (2003)
• [Publications] Kitano J, Nishida M, Itsukaichi Y, Minami I, Ogawa M, Hirano T, Mori Y, Nakanishi S: "Direct interaction and functional coupling between metabotropic rec type 1 and voltage-sensitive Ca_v2.1 Ca^<2+> channel."J.Biol.Chem.. 278. 25101-25108 (2003)
• [Publications] Nishida M, Sugimoto K, Hara Y, Mori E, Morii T, Kurosaki T, Mori Y: "Amplification of receptor signaling by Ca^<2+> entry-mediated translocation and activation of phospholipase Cγ2 in B lymphocytes."EMBO J.. 22. 4677-4688 (2003)
• [Publications] Sugimoto K, Nishida M, Otsuka M, Ohkubo K, Mori Y, Morii T: "Novel real time sensors to quantitatively assess in vivo inositol 1,4,5-trisphosphate production in intact cells."Chem.Biol.. (in press).
• [Publications] Koichi Nakajo, Yasushi Okumura: "Development of transient outward currents coupled with Ca^<2+>-induced Ca^<2+> release mediates oscillatory membrane potential in ascidian muscle cell"Journal of Neurophysiology. (in press).
• [Publications] Ian A.Meinertzhagen, Patrick Lemaire, Yasushi Okamura: "The neurobiology of the ascidian tadpole larva : recent developments in an ancient chordate."Annual Reviews of Neuroscience. (in press).
• [Publications] Izumi-Nakaseko, H., Yamaguchi, S., Ohtsuka, Y., Ebihara, T., Adachi-Akahane, S., Okamura, Y.: "DHP-insensitive L-type-like Ca channel of ascidian acquires sensitivity to DHP with single amino acid change in domain III P-region."FEBS letters. 549. 67-71 (2003)
• [Publications] Okamura, Y., Izumi-Nakaseko, H., Nakajo, K., Ohtsuka, Y., Ebihara, T.: "The ascidian dihydropyridine-resistant calcium channel as the prototype of chordite L-type calcium channel."Neurosignals. 12・3. 142-158 (2003)
• [Publications] Sam Dylan More, Jiri Hudececk, Tsuneo Urisu: "Hydrophobic/hydrophilic interactions of cytochrome C with functionalized self-assembled monolayers on silicon"Surf.Sci.. 532-535. 993-998 (2003)
• [Publications] Zhi-Hong Wang, T.Urisu, S.Nanbu, J.Maki, G.Ranga Rao, M.Aoyagi, H.Watanabe, K.Ooi: "Three pairs of doublet bands assigned to SiH_2 scissoring modes observed in H_2O-induced oxidation of Si(100) surfaces."Phys.Rev.. B69. 045309-045315 (2004)
• [Publications] C.Wang, S.D.More, Z.Wang, S.Yamamura, Y.Nonogaki, T.Urisu: "Patterning SiO_2 thin films using synchrotron radiation stimulated etching with a Co contact mask"J.Vac.Sci.Technol.. B21. 818-822 (2003)
• [Publications] S.Yamamura, S.Yamauchi, S.Watanabe, M.Tabe, T.Kasai, Y.Nonogaki, T.Urisu: "Infrared Reflection Absorption Spectroscopy Using CoSi_2 Buried Metal Layer Substrates Made by Wafer-Bonding"Jpn.J.Appl.Phys.. 42. 3942-3945 (2003)
• [Publications] C.Wang, T.Urisu: "Synchrotron radiation stimulated etching SiO_2 thin films with a Co contact mask for the area-selective deposition of self-assenbled monolayer."Jpn.J.Appl.Phys.. 42. 4016-4019 (2003)
• [Publications] Yong-Hoon Kim, R.Tero, M.Takizawa, T.Urisu: "Characterization of dipalmitoylphosphatidylcholine (DPPC)/cholesterol Langmuir-Blodgett monolayers investigated by AFM and FT-IR."Jpn.J.Appl.Phys.. (In printing). (2004)
• [Publications] Md.Mashiur Rahman, R.Tero, T.Urisu: "Shrinking of spin-on-glass films induced by synchrotron radiation and its application to the 3-D microfabrications"Jpn.J.Appl.Phys.. (In printing). (2004)
• [Publications] R.Tero, M.Takizawa, Yan-Jun Li, M.Yamazaki, T.Urisu: "Deposition of phospholipid layers on SiO_2 surface modified by alkyl-SAM islands."Appl.Surf.Sci.. (In printing).
• [Publications] Yan-Jun Li, R.Tero, T.Nagasawa, T.Nagata, T.Urisu: "Deposition of undecenoic acid self-assembled monolayers on H-Si(111) surfaces studied by AFM."Appl.Surf.Sci.. (In printing).
• [Publications] M.Takizawa, Yong-Hoon Kim, T.Urisu: "Deposition of DPPC monolayers by Langmuir-Blodget method on SiO_2 surfaces covered by octadecyltrichlorosilane self-assembled monolayer islands."Chem.Phys.Lett.. 385. 220-224 (2004)
• [Publications] T.Kanbara, K.Shibata, S.Fujiki, Y.Kubozono, S.Kashino, T.Urisu, M.Sakai, A.Fujiwara, R.Kumashiro, K.Tanigaki: "N-channel field effect transistors with fullerene thin films and their application to a logic gate circuit."Chem.Phys.Lett.. 379. 223-229 (2003)
• [Publications] 宇理須恒雄: "ナノテクノロジーハンドブック(4・4ナノエッチングプロセス)"(株)オーム社. 6 (2003)
• [Publications] K.Danov, R.Danev, K.Nagayama: "Reconstruction of the electric charge density in thin films from the contrast transfer function measurements"Ultramicroscopy. 90. 85-95 (2002)
• [Publications] S.Sugitani, K.Nagayama: "Complex Observation in Electron Microscopy. III. Inverse Theory of Observation-scheme Dependent Information Transfer"J. Phys. Soc. Jpn.. 71. 744-756 (2002)
• [Publications] M.Takano, K.Nagayama, A.Suyama: "How the All-Atom Simulation and the Ising-Based Theory Reconcile with Each Other on the Helix-Coil Transition"J. Biol. Phys.. 28. 155-161 (2002)
• [Publications] M.Takano, K.Nagayama, A.Suyama: "Investigating a link between all-atom model simulation and the Ising-Based theory on the Helix-Coil transition : Equilibrium Statistical Mechanics"J. Chem. Phys.. 116. 2219-2228 (2002)
• [Publications] R.Danev, K.Nagayama: "A Novel Phase-contrast Transmission Electron Microscopy Producing High-contrast Topographic Images of Weak Objects"J. Biol. Phys.. 28. 627-635 (2002)
• [Publications] 永山國昭, 三井利夫: "生物物理-蛋白質から分子機械へ"物性研究. 77. 655-683 (2002)
• [Publications] 永山國昭: "情報高分子の人為的創出"高分子. 51. 427 (2002)
• [Publications] 永山國昭: "50年前の『科学』断章"科学. 72. 977-978 (2002)
• [Publications] 永山國昭: "2002年度ノーベル化学賞-クルト・ヴュートリッヒの人と業績"化学と工業. 55. 1336-1339 (2002)
• [Publications] Yasuda M, Shimizu S, Uhinata K, Naito S, Tokuyama S, Mori Y, Kiuchi Y, Yamamoto T: "The differential roles of intercellular adhesion molecule-1 and E-selectin in polymorphonuclear leukocyte-induced angiogenesis"Am. J. Physiol. (Cell Physiol.). 282. C917-C925 (2002)
• [Publications] Morii T, Sugimoto K, Makino K, Otsuka M, Imoto K, Mori Y: "A new fluoresent biosensor for inositol trisphosphate"J. Am. Chem. Soc.. 124. 1138-1139 (2002)
• [Publications] Mori Y, Wakamori M, Miyakawa T, Hermosura M, Hara Y, Nishida M, Hirose K, Mizushima A, Okada T, Kurosaki M, Mori E, Gotoh K, Fleig A, Penner R, Iino M, Kurosaki T: "TRP1 regulates capacitative Ca^<2+> entry and Ca^<2+> release from endoplasmic reticulum in B lymphocytes"J. Exp. Med.. 195. 673-681 (2002)
• [Publications] Montell C, Birnbaumer L, Flokerzi V, Bindels RJ, Bruford EA, Caterina MJ, Clapham DE, Harteneck C, Heller S, Julius D, Mori Y, Penner R, Prawitte D, Scharenberg AM, Schultz G, Shimizu N, Zhu MX: "A unified nomenclature for the superfamily of TRP cation channels"Mol. Cell. 9. 229-231 (2002)
• [Publications] Inoue R, Mori Y: "Molecular candidates for capacitative and non-capacitative Ca^<2+> entry in smooth mus In "Role of the Sarcoplasmic Reticulum in Smooth Muscle""Novartis Foundation Symposium Volume 246 (ed. Eisner D, Wiley and Sons Ltd., Chichester, UK). 246. 81-90 (2002)
• [Publications] Dos Santos RG, Van Renterghem C, Martin-Moutot N, Mansuel P, Sampieri F, Diniz C, Mori Y, De Lima: "Dos Santos RG, Van Renterghem C, Martin-Moutot N, Mansuel P, Sampieri F, Diniz C, Mori Y, De Lima"J. Biol. Chem.. 277. 13856-13862 (2002)
• [Publications] Matsushita K, Wakamori M, Rhyu IJ, Arii T, Oda S, Mori Y, Imoto K: "Bidirectional alterations in cerebellar synaptic transmission of tottering and rolling Ca^<2+> channel mutant mice"J. Neurosci.. 22. 4388-4398 (2002)
• [Publications] Kato K, Wakamori M, Mori Y, Imoto K, Kitamura K: "Inhibitory effects of cilnidipine on peripheral and brain N-type Ca^<2+> channels expressed in BHK cells"Neuropharmacol. 42. 1099-1108 (2002)
• [Publications] Cornet V, Bichet D, Sandoz G, Marty I, Brocard J, Bourinet E, Mori Y, Villaz M, De Waard M.: "Multiple ER retention determinants control the cell surface targeting of voltage-dependent P/Q calcium channels"Eur. J. Neurosci.. 16. 883-895 (2002)
• [Publications] Arikkath J, Felix R, Ahern C, Chen CC, Mori Y, Song I, Shin H-S, Coronado R, Campbell KP: "Molecular characterization of a two-domain form of the neuronal voltage-gated P/Q-type calcium channel α_12.1 subunit"FEBS Lett.. (in press). (2002)
• [Publications] Mori Y, Nishida M, Ino M, Yoshinaga T, Niidome T, Sawada K: "Mice lacking the α_<1B> subunit (Cav 2.2) reveals a predominant role of N-type Ca^<2+> channels in the sympathetic regulation of circulatory system"Trends Cardiovasc. Med.. 12. 270-274 (2002)
• [Publications] 森泰生, 吉田卓史, 原雄二: "脳における多様な非選択性カチオンチャネル"生体の科学(特集 一価イオンチャネル). 53. 316-322 (2002)
• [Publications] 井上隆司, 伊東祐之, 森泰生: "標的蛋白質からみた創薬-急速に拡大するTRP蛋白質ファミリーと新しい創薬の可能性(The TRP proteins, a rapidly expanding Ca^<2+> entry channel family and a new molecular target for drug development)"日本臨床. 60. 18-24 (2002)
• [Publications] 原雄二, 森泰生: "TRPチャネル"医学のあゆみ(イオンチャネルの最前線). 201. 999-103 (2002)
• [Publications] Nakajo K, Katsuyama Y, Ono F, Ohtsuka Y, Okamura Y: "Identification, functional characterization and developmental expression of the ascidian Kv4-class potassium channel"Neurosci Res. 45(1). 59-70 (2003)
• [Publications] Ebihara T, Komiya Y, Izmi-Nakaseko H, Adachi-Akahane S, Okabe S, Okamura Y: "Coexpression of a Cav1.2 protein lacking an N-terminus and the first domain specifically suppresses L-type calcium channel activity"FEBS Lett. 529. 203-207 (2002)
• [Publications] Okada T, Katsuyama Y, Ono F, Okamura Y: "The development of three identified motor neurons in ascidian embryo"Dev Biol. 244. 278-292 (2002)
• [Publications] Katsuyama Y, Matsumoto J, Okada T, Ohtsuka Y, Cheng L, Okado H, Okamura Y: "Regulation of synaptotagmin gene expression during ascidian embryogenesis"Dev Biol. 244. 293-304 (2002)
• [Publications] 岡村 康司: "電位依存性Na+チャネル(分子と機能の多様性に関する知見)"医学のあゆみ. 201. 971-976 (2002)
• [Publications] Zhi-Hong Wang, Hideyuki Noda, Youichi Nonogaki, Norikuni Yabumoto, Tsuneo Urisu: "IR linewidth broadening at nearly ideal H-termination region on Si(100) surfaces"Surf. Sci.. 502-503. 86-90 (2002)
• [Publications] Sam Dylan More, Harald Graaf, Michael Baune, Changshun Wang, Tsuneo Urisu: "Influence of substrate roughness on the formation of aliphatic self-assembled monolayers(SAMs) on silicon(100)"Jpn. J. Appl. Phys.. 41. 4390-4394 (2002)
• [Publications] Zhi-Hong Wang, Hideyuki Noda, Youichi Nonogaki, Norikuni Yabumoto, Tsuneo Urisu: "Hydrogen diffusion and chemical reactivity with water on nearly ideally H-terminated Si(100) surface"Jpn. J. Appl. Phys.. 41. 4275-4278 (2002)
• [Publications] T.Takahashi, J.Sugiura, Kuniaki Nagayama: "Comparison of all atom, continuum, and linear fitting empirical models for charge screening effect of aqueous medium surrounding a protein molecule"J. Chem. Phys. 116. 8232-8237 (2002)
• [Publications] S.Aono, T.Kato, M.Matsuki, H.Nakajima, T.Ohta, T.Uchida, T.Kitagawa: "Resonance Raman and ligand binding studies of the oxygen sensing signal transducer protein HemAT from Bacillus subtilis"J. Biol. Chem.. 277(16). 13528-13538 (2002)
• [Publications] 青野重利: "一酸化炭素センサーとして機能する転写調節因子CooAの構造と機能"Molecular Medicine. 40(2). 160-164 (2003)
• [Publications] 青野重利: "微生物におけるCOの受容システム"医学のあゆみ. 201(8). 733-736 (2002)
• [Publications] 青野重利: "一酸化炭素による遺云子発現制御:COセンサーとして機能する云転写調節因子CooAの構造と機能"化学と生物. 40(3). 206-210 (2002)
• [Publications] N.Makihara: "pH-Selective Hydrogenation of Water-soluble Carbon Compounds and Alkenes with [Cp*IrIII(H2O)3]2+(Cp*=h5C5H) as A catlyst Precusor in Very Acidic Media"Organometallics. 20. 497-500 (2001)
• [Publications] H.Ohtsu: "Characterization of Imidazolate-Bridged Dinuclear and Mononuclear Hydroperoxo Complexes"Inort.Chem.. 40. 3200-3207 (2001)
• [Publications] N.Haruta: "Protein Conformation Change of Myoglobin upon Ligand Binding Probed by Ultraviolet Resonance Raman Spectroscopy"Biochemistry. 40. 6956-6963 (2001)
• [Publications] S.Herold: "Peroxynitrite Isomerization Catalyzed by His64 Myoglobin Mutants"J.Am.Chem.Soc.. 123. 4085-4086 (2001)
• [Publications] T.Tomita: "Elucidation of the Differences between the 430 and 455-nm Absorbing Forms of P450-Isocyanide Adducts by Resonance Raman Spectroscopy"J.Biol.Chem.. 276. 36261-36267 (2001)
• [Publications] I.Hara: "Oxidative Modification of Thyptophan-43 in the Heme Vicinity of the F43W/H64L Myoglobin Mutant"J.Biol.Chem.. 276. 36067-36070 (2001)
• [Publications] Sandoz G.: "Distinct properties and differential β subunit regulation of two C-terminal isoforms of the P/Q-type Ca2+ channel α1A subunit"Eur.J.Neurosci.. 14. 987-997 (2001)
• [Publications] R.Danev: "Transmission Electron Microsocpy with Zernike Phase Plate"Ultrarnicroscopy. 88. 243-252 (2001)
• [Publications] Mori Y.: "Dissecting receptor-mediated Ca2+ influx pathways : TRP channels and their native counterparts"Jpn.J.Pharmacol. 87. 245-252 (2001)
• [Publications] N.Takezoe: "Design and construction of UVSOR-BL4A2 beam line for nano-structure processing"Nucl.Instrum.Methods.. A467, Part2. 1279-1281 (2001)
• [Publications] Y.Nonogaki: "STM observation of surface nanostructures on Si(111) formed after synchrotron radiation stimulated cleaning"Transactions MRS Japan. 26. 751-754 (2001)
• [Publications] Y.Nonogaki: "Nanostructure formation on Si(111) surface assisted by synchrotron radiation illumination-Characterization by scanning tunneling microscopy"J.Electron spectroscopy. 119. 241-246 (2001)
• [Publications] S.Ogo: "pH-Dependent Transfer Hydrogenation, Reductive Amination, and Dehalogenation of Water-Soluble Carbonyl Compounds and Alkyl halides Promoted by CP*lr Complexes"Organomatellics. 20. 4903-4910 (2001)
• [Publications] S.Ogo: "pH-Dependent H2-Activation Cycle to Reducection of Nitrate Ion by Metal Complexes"J.Am.Chem.Soc.. 124. 597-601 (2002)
• [Publications] Y.Watanabe: "Asymmetric Oxidation of Sulfides and olefins by Myoglobin Mutants"Rev.Heteroatom Chem.. 22. 135-150 (2001)
• [Publications] J.L.Que: "Oxygenase Pathways : Oxo, Peroxo, and Superoxo"Science. 292. 651-653 (2001)
• [Publications] S.Ozaki: "Investigations of the Roles of the Distal Heme Environment and the Proximal Heme Iron Ligand in Peroxide Activation by Heme Enzymes via Molecular Engineering of Myoglobin"Acc.Chem.Res.. 34. 818-825 (2001)
• [Publications] Y.Watanabe: "Two-Electron Oxidized Derivatives of Fe (III) Porphyrins"J.Biol.Inorg.Chem.. 6. 846-856 (2001)
• [Publications] Okada, T.: "The form of neurons in the ascidian central nervous system of the tadpole larva of Ciona"Biol.Bull.. 200. 252-256 (2001)
• [Publications] Ohtsuka, Y.: "Changes in gelsolin expression during ascidian metamorphosis"Development Gene & Evolution. 211. 252-256 (2001)
• [Publications] K.Danov: "Electric charging of thin films measured using the constrast transfer function"Ultramicroscopy. 87. 45-54 (2001)
• [Publications] D.Tanooka: "Color-imaging ellipsometer : High-Seed characterization of in-plane distribution of film thickness at nano-scale"Jpn.J.Appl.Phys.. 40. 877-880 (2001)
• [Publications] R.Danev: "Complex Observation in Electron Microscopy. II. Direct Visualization Phases and Amplitudes of Exit Wave Functions"J.Phys.Soc.Jpn.. 70. 696-702 (2001)
• [Publications] K.Murata: "Asymmetric arrangement of auxiliary subunits of skeletal muscle voltage-gated L-type Ca2+ channel"Biochim.Biophys.Res.Commun.. 282. 284-291 (2001)
• [Publications] Ino M.: "Functional disorders of the sympathetic nervous system in mice lacking the α 1B subunit (Cav 2.2) of N-tupe calclum channel"Proc.Natl.Acad.Sci.USA. 98. 5323-5328 (2001)
• [Publications] Kato Y.: "Evidence in support of a four transmembrane-pore-transmembrane topolpgy model for the Arabidopsis thaliana Na+/K+ translocation AtHKT1 protein, a member of the superfamily of K+ transporters"Proc.Natl.Acad.Sci.USA. 98. 6488-6493 (2001)
• [Publications] Hatakeyama S.: "Differential nociceptive responses in mice lacking subunit the α 1B subunit of N-type Ca2+ channels"Neuroreport. 12. 2423-2427 (2001)
• [Publications] Kinoshita M.: "Binding of G α 0 N-terminus is responsible for the voltage-resisitant inhibition of α 1B (P/Q-type, Cav 2.1) Ca2+ channels"J.Biol.Chem.. 276. 28731-28783 (2001)
• [Publications] Kang MG.: "Biochemical and biophysical evidence for γ2 subunit association with neuronal voltaae-actlvated Ca2+ channels"J.Biol.Chem.. 276. 32917-32924 (2001)
• [Publications] Kanki H.: "Activation of inisitol 1, 4, 5-trlsphosphate receptor is essential for the opening of mouse TRP5 channels"Mol.Pharmacol.. 60. 989-998 (2001)
• [Publications] Ohtsuka, Y: "Induction of ascidian peripheral neuron vegetal blastomeres"Dev.Biol.. 239(1). 107-117 (2001)
• [Publications] Murata, Y.: "Primary structure, developmental expression and functional properties of an inward rectifier K+ channel of the tunicate"Receptors and Channels. 7. 387-399 (2001)
• [Publications] Ohtsuka, Y.: "Induction of ascidian peripharal neuron by vegetal blastomeres"Dev.Biol.. 239. 107-117 (2001)
• [Publications] Meinertzhagen, I.A.: "The ascidian larval nervous system : the chordate brain from its small beginnings"Trends in Neurosci.. 24(7). 401-410 (2001)