Grant-in-Aid for Scientific Research on Priority Areas
|Allocation Type||Single-year Grants|
|Research Institution||Kyoto University|
HAYAME Masanori Kyoto University, Institute for Virus Research, Professor, ウイルス研究所, 教授 (40072946)
IDO Eiji Kyoto University, Institute for Vitus Research, Assistant professor, ウイルス研究所, 助手 (70183176)
榎瀬 良美 京都大学, ウイルス研究所, 日本学術振興会特別研究員(PD)
|Project Period (FY)
2002 – 2005
Completed(Fiscal Year 2005)
|Budget Amount *help
¥64,000,000 (Direct Cost : ¥64,000,000)
Fiscal Year 2005 : ¥15,000,000 (Direct Cost : ¥15,000,000)
Fiscal Year 2004 : ¥15,000,000 (Direct Cost : ¥15,000,000)
Fiscal Year 2003 : ¥16,000,000 (Direct Cost : ¥16,000,000)
Fiscal Year 2002 : ¥18,000,000 (Direct Cost : ¥18,000,000)
|Keywords||AIDS / DNA vaccine / AIDS vaccine / SIV / SHIV / monkev / mucosal infection / mucosal immunity / HIV / ウイルス体内分布 / 分化障害 / SIV / γδ細胞 / NK活性 / 経粘膜感染 / アカゲザル / 樹状細胞 / マクロファージ|
In order to clarify the mechanisms of mucosal infection and mucosal immunity of HIV-1, we have tried to establish the monkey experimental model system of mucosal infection using SIV and SHIV and to see the virus spread and immune response for protection. Results obtained are followed.
1. Immunities and protective effect of nonpathogenic SHIV against mucosal infection (2001).
Involvement of 7 5 T and NK as well as acquired immunities was shown in the protection against intrarectal attack infection of pathogenic SHIV in the monkeys immunized with nef-deleted nonpathogenic SHIV.
2. Virus population through SIV intravaginal infection (2002).
Virus population was restricted when it passed through mucosa and it had no relation to viral cell tropisms.
3. Virus spread and immune responses at the early phase of intrarectal infection of acute pathogenic SHIV (2003).
Immediately after intrarectal infection, the virus spread in a whole body, not replicating at the mucosal sites. The CD4 cell decrease was prominent especially in thymus in which impairment of T cell maturation was ex vivo demonstrated.
4. Virus spread and immune responses at the early phase of intrarectal infection of low pathogenic SHIV (2004).
Virus replicated and induced CD4 cell depletion at the rectal sites, and gradually spread to a whole body in which CD4 depletion was slight.
5. Vaccination with SHIV DNA which produces noninfectious virions protected mucosal infection (2005).
SHIV mutant DNA lacking packaging ability induced protective effect against intrarectal attack infection of acute pathogenic SHIV.
Close association of virus spread and immure response with prognosis had been suggested in HIV1 infection. This study experimentally proved it by using monkeys. The obtains results are important and useful for develonment of effeetive vaepine develonment