Phosphorylation-dependent regulation of splicing and transport of mRNA

• Project/Area Number: 14035102
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Tokyo University of Agriculture and Technology (2006); Tokyo Medical and Dental University (2002-2005)
• Principal Investigator: HAGIWARA Masatoshi Tokyo University of Agriculture and Technology, Laboratory of Gene Expression School of Biomedical Science & Dept Functional Genomics Medical Research Institute, Professor (10208423)
• Co-Investigator(Kenkyū-buntansha): TANI Tokio Kumamoto University, Faculty of Science, Professor (80197516)
• Project Period (FY): 2002 – 2006
• Project Status: Completed (Fiscal Year 2006)
• Budget Amount: ¥72,100,000 (Direct Cost: ¥72,100,000); Fiscal Year 2006: ¥13,400,000 (Direct Cost: ¥13,400,000); Fiscal Year 2005: ¥13,400,000 (Direct Cost: ¥13,400,000); Fiscal Year 2004: ¥15,100,000 (Direct Cost: ¥15,100,000); Fiscal Year 2003: ¥15,100,000 (Direct Cost: ¥15,100,000); Fiscal Year 2002: ¥15,100,000 (Direct Cost: ¥15,100,000)
• Keywords: Alternative splicing / mRNA export / Aly / REF / SF1-U2AF / CBP20 / EJC / asd-1 / 転写 / スプライシング共役系 / SRPIN340 / SRPK / エキソンスキッピング / cellular codes / インシュリン / 構成的スプライシング / SF1-U2AF複合体 / RNA結合蛋白質 / SRタンパク質 / Clk1 / Sty / 蛋白リン酸化酵素阻害剤 / U2AF / RSドメイン / Clk / mRNAプロセシング / 特異的阻害剤 / mRNA輸送 / RS蛋白 / リン酸化酵素

Research Abstract

1) RNA export factor, REF is a component of the exon junction complex (EJC) that is deposited on mRNA in a splicing-dependent manner, and targets spliced mRNA for export. To analyze the effect of transcription on the EJC formation, we developed a coupled in vitro system for examining transcription/splicing reactions. Analysis of the RNA-binding complexes revealed that REF associated with β-globin mRNA at regions other than the EJC deposition site. Comparison between RNA polymerase II and T7 transcription and further analysis show that the deposition of REF apart from the EJC is dependent on 5' cap structure, but not splicing. Excess amounts of m7GpppG cap analog reduced REF binding to intronless mRNA, and a co-immunoprecipitation experiment revealed that REF interacts with the cap-binding protein CBP20. The export of Cy3-labeled intronless β-globin mRNA from nuclei of HeLa cells was enhanced by co-injection of CBP20 and REF. Thus, REF recruited by CBP20 may play a stimulatory role to e xport the capped intronless mRNAs.
2) As many as two thirds of human genes have alternative mRNA isoforms. What is a spatiotemporal expression profile of each isoform? How are so many genes regulated in vivo? Regulation mechanisms of alternative splicing, however, have been studied mostly in vitro or in cultured cells.
We have developed a transgenic alternative splicing reporter system that visualizes expression profiles of mutually exclusive alternative exons of a nematode C. elegans at a single cell level in vivo (nature methods, 2006). With this reporter system, we have visualized spatiotemporal profiles of tissue-specific and developmentally regulated alternative splicing events in living worms. By isolating and analyzing mutant worms defective in the color profiles, we have identified transacting factors and cis-elements involved in the splicing regulation (Mol Cell Biol, 2007; Genes Dev, 2008). Through these studies, we are coming to realize that molecular mechanisms of the alternative splicing regulation are conserved throughout metazoan evolution. Our reporter system will further elucidate expression profiles and regulation mechanisms of alternative splicing in vivo.
3) To identify genes involved in the mechanism to ensure ordered 5' to 3' exon joining in constitutively spliced pre-mRNAs, we isolated and characterized exon-skipping mutants in fission yeast. The results showed that co-transcriptional recognition of a nascent pre-mRNA by the SF1-U2AF^<59>-U2AF^<23> complex is essential for ordered exon joining in constitutive splicing. In addition, the analyses of the mRNA export mutants revealed that a subset of mRNAs in yeast is exported from the nucleus through transient association with the nucleolus. Our results contributed elucidation of molecular mechanisms of pre-mRNA splicing and nuclear mRNA export.

Research Products

• [Journal Article] Participation of XPB/Ptr8p, a component of TFIIH, in nucleocytoplasmic transport of mRNA in fission yeast (2007)
  • Author(s): Fumitaka Mizuki
  • Journal Title: Genes to Cells 12 Pages: 35-47
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Mutations in the SF1-U2AF59-U2AF23 complex cause exon skipping in Schizosaccharomyces pombe (2007)
  • Author(s): Noriko Haraguchi
  • Journal Title: J. Biol. Chem. 282 Pages: 2221-2228
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Effect of cisplatin treatment on speckled distribution of a serine/arg inine-rich nuclear protein CROP/Luc7A (2007)
  • Author(s): Umehara, H., Nishii, Y., Morishima, M., Kakehi, Y., Kioka, N., Amachi, T., Koizumi, J., Hagiwara, M. & Ueda, K.
  • Journal Title: Biochem. Biophys. Res. Comm. 301 Pages: 324-329
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The interaction between cap-binding complex and REF is required for intronless mRNA export. (2007)
  • Author(s): Nojima, T
  • Journal Title: J. Biol. Chem. (in press)
• [Journal Article] Shortened Life-Spans of Biotech Pioneer Patents in Japan : A Lesson from the DNA Chip. (2007)
  • Author(s): Aida, T.
  • Journal Title: Nature Biotech. (in press)
• [Journal Article] Mutations in the SF1-U2AF59-U2AF23 complex cause exon skipping in Schizosaccharomyces pombe (2007)
  • Author(s): Haraguchi.N
  • Journal Title: J. Biol. Chem. 282 Pages: 2221-2228
• [Journal Article] Participation of XPB/Ptr8p, a component of TFIIH, in nucleocytoplasmic transport of mRNA in fission yeast (2007)
  • Author(s): Mizuki.F
  • Journal Title: Genes to Cells 12 Pages: 35-47
• [Journal Article] Utilization of host SR protein kinases and RNA-splicing machinery during viral replication (2006)
  • Author(s): Fukuhara T, Hosoya T, Shimizu S, Sumi K, Oshiro T, Yoshinaka Y, Suzuki M, Yamamoto N, Herzenberg LA, Herzenberg LA, Hagiwara M
  • Journal Title: Proc Natl Acad Sci USA 103(30) Pages: 11329-11333
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] A spliceosomal intron-binding protein, IBP160, links position-dependent assembly of intorn-encoded box C/C snoRNP to pre-mRNA splicing. (2006)
  • Author(s): Hirose T., Ideue, T., Nagai, M., Hagiwara. M., Shu, M., Steitz, J. A
  • Journal Title: Mol. Cell 23(5) Pages: 673-684
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] A molecular neuroethological approach for identifying and characterizing a cascade of behaviorally regulated genes. (2006)
  • Author(s): Wada, K., Howard, J. T., McConnell, P., Whitney, O., Lints, T., Rivas, M. V. Horita, H., Patterson, M. A., White, S. A., Scharff, C., Haesler, S., Zhao, S., Sakaguchi, H., Hagiwara, M.. Shiraki, T., Hirozane-Kishikawa, T., Skene, P., Hayashizaki, Y., Carninci, P., Jarvis, E. D.
  • Journal Title: Proc Natl Acad Sci USA. 103(41) Pages: 15212-15217
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] A transgenic reporter reveals cell-type-specific expression profiles and regulation mechanisms of alternatively-spliced exons in vivo. (2006)
  • Author(s): Kuroyanagi, H., Kobayashi, T., Mitani, S., Hagiwara. M.
  • Journal Title: Nature Methods 3 Pages: 909-915
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] A novel histone・exchange factor, protein phosphatase 2C_y, mediates the exchange and dephosphorylation of H2A/H2B. (2006)
  • Author(s): Kimura, H., Takizawa N., Allem, E., Hori, T., Iborra, F. J., Nozaki, N., Muraki, M., Hagiwara, M. ., Krainer, A. R., Fukagawa, T., Okawa, K.
  • Journal Title: J. Cell Biol. 175 Pages: 389-400
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Nucleocytoplasmic transport of fluorescent mRNA in living mammalian cells : Nuclear mRNA export is coupled to ongoing gene transcription (2006)
  • Author(s): Kazuaki Tokunaga
  • Journal Title: Genes to Cells 11 Pages: 305-317
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Imaging of single mRNA molecules movement within a living cell nucleus (2006)
  • Author(s): Hisashi Tadakuma
  • Journal Title: Biochem. Biophys. Res. Commun. 344 Pages: 772-779
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Visual screening for localized RNAs in yeast revealed novel RNAs at the bud-tip (2006)
  • Author(s): Tomoko Andoh
  • Journal Title: Biochem. Biophys. Res. Commun. 351 Pages: 999-1004
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] A novel histone-exchange factor, protein phosphatase 2Cγ, mediates the exchange and dephosphorylation of H2A/H2B. (2006)
  • Author(s): Kimura, H., Takizawa N., Allemand, E., Hori, T., Iborra, F. J., Nozaki, N., Muraki, M., Hagiwara, M., Krainer, A. R., Fukagawa, T., Okawa, K.
  • Journal Title: J. Cell Biol. 175 Pages: 389-400
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A transgenic reporter reveals cell-type-specific expression profiles and regulation mechanisms of alternatively-spliced exons in vivo. (2006)
  • Author(s): Kuroyanagi, H., Kobayashi, T., Mitani, S., Hagiwara, M.
  • Journal Title: Nature Methods 3 Pages: 909-915
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A molecular neuroethological approach for identifying and characterizing a cascade of behaviorally regulated genes. (2006)
  • Author(s): Wada, K., Howard, J. T., McConnell, P., Whitney, O., Lints, T., Rivas, M. V. Horita, H., Patterson, M. A., White, S. A., Scharff, C., Haesler, S., Zhao, S., Sakaguchi, H., Hagiwara, M., Shiraki, T., Hirozane-Kishikawa, T., Skene, P., Hayashizaki, Y., Carninci, P., Jarvis, E. D.
  • Journal Title: Proc Natl Acad Sci USA. 103(41) Pages: 15212-15217
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A spliceosomal intron-binding protein, IBP160, links position-dependent assembly of intorn-encoded box C/C snoRNP to pre-mRNA splicing. (2006)
  • Author(s): Hirose T., Ideue, T., Nagai, M., Hagiwara, M., Shu, M., Steitz, J. A.
  • Journal Title: Mol. Cell. 23(5) Pages: 673-684
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Utilization of host SR protein kinases and RNA-splicing machinery during viral replication. (2006)
  • Author(s): Fukuhara T, Hosoya T, Shimizu S, Sumi K, Oshiro T, Yoshinaka Y, Suzuki M, Yamamoto N, Herzenberg LA, Herzenberg LA, Hagiwara M
  • Journal Title: Proc Natl Acad Sci USA. 103(30) Pages: 11329-11333
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A novel histone-exchange factor, protein phosphatase 2Cγ, mediates the exchange and dephosphorylation of H2A/H2B. (2006)
  • Author(s): Kimura, H.
  • Journal Title: J. Cell Biol. 175(3) Pages: 389-400
• [Journal Article] A transgenic reporter reveals cell-type-specific expression profiles and regulation mechanisms of alternatively-spliced exons in vivo. (2006)
  • Author(s): Kuroyanagi, H.
  • Journal Title: Nature Methods 3 Pages: 909-915
• [Journal Article] A molecular neuroethological approach for identifying and characterizing a cascade of behaviorally regulated genes. (2006)
  • Author(s): Wada, K.
  • Journal Title: Proc Natl Acad Sci USA. 103(41) Pages: 15212-15217
• [Journal Article] A spliceosomal intron-binding protein, IBP160, links position-dependent assembly of intorn-encoded box C/C snoRNP to pre-mRNA splicing. (2006)
  • Author(s): Hirose T.
  • Journal Title: Mol. Cell. 23(5) Pages: 673-684
• [Journal Article] Utilization of host SR protein kinases and RNA-splicing machinery during viral replication. (2006)
  • Author(s): Fukuhara T
  • Journal Title: Proc Natl Acad Sci USA. 103(30) Pages: 11329-11333
• [Journal Article] Visual screening for localized RNAs in yeast revealed novel RNAs at the bud-tip (2006)
  • Author(s): Andoh.T
  • Journal Title: Biochem. Biophys. Res. Commun 351 Pages: 999-1004
• [Journal Article] Imaging of single mRNA molecules movement within a living cell nucleus (2006)
  • Author(s): Tadakuma.H
  • Journal Title: Biochem. Biophys. Res. Commun 344 Pages: 772-779
• [Journal Article] Nucleocytoplasmic transport of fluorescent mRNA in living mammalian cells : Nuclear mRNA export is coupled to ongoing gene transcription (2006)
  • Author(s): Tokunaga.K
  • Journal Title: Genes to Cells 11 Pages: 305-317
• [Journal Article] Nucleocytoplasmic transport of fluorescent mRNA in living mammalian cells : nuclear mRNA export is coupled to ongoing gene transcription (2006)
  • Author(s): Tokunaga, K.
  • Journal Title: Genes to Cells 11 Pages: 305-317
• [Journal Article] Bio-object, a stochastic simulator for post-transcriptional regulation. (2005)
  • Author(s): Ohki, N, Hagiwara. M
  • Journal Title: Bioinformatics. 21 Pages: 2478-2487
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Alternative splicing : a new drug target of the post・genome era (2005)
  • Author(s): Hagiwara, M.
  • Journal Title: Biochim Biophys Acta. 1754 Pages: 324-331
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Hspl6p is required for thermotolerance in nuclear mRNA export in fission yeast Schizosaccharomyces pombe (2005)
  • Author(s): Jun-ichi Yoshida
  • Journal Title: Cell Structure and Function 29 Pages: 125-138
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Single molecule imaging of mRNA splicing (2005)
  • Author(s): Yo Ishihama
  • Journal Title: Nucleic Acids Symp. Ser. 49 Pages: 209-210
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Alternative splicing : a new drug target of the post-genome era (2005)
  • Author(s): Hagiwara, M.
  • Journal Title: Biochim Biophys Acta. 1754 Pages: 324-331
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Bio-Object, a stochastic simulator for post-transcriptional regulation. (2005)
  • Author(s): Ohki, N., Hagiwara, M.
  • Journal Title: Bioinformatics 21 Pages: 2478-2487
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Bio-object, a stochastic simulator for post-transcriptional regulation. (2005)
  • Author(s): Ohki, N
  • Journal Title: Bioinformatics 21 Pages: 2478-2487
• [Journal Article] Alternative splicing : a new drug target of the post-genome era. (2005)
  • Author(s): Hagiwara, M.
  • Journal Title: Biochim Biophys Acta. 1754 Pages: 324-331
• [Journal Article] Hsp16 is required for thermotolerance in nuclear mRNA export in fission yeast Schizosaccharomyces pombe (2005)
  • Author(s): Yoshida, J.
  • Journal Title: Cell Structure and Function 29 Pages: 125-138
• [Journal Article] Bio-Object, a stochastic simulator for post-transcriptional regulation. (2005)
  • Author(s): Ohki, N., Hagiwara, M.
  • Journal Title: Bioinformatics (in press)
• [Journal Article] Hsp 16p is required for thermotolerance in nuclear mRNA export in fission yeast Schizosaccharomyces pombe (2005)
  • Author(s): Jun-ichi Yoshida, Tokio Tani
  • Journal Title: Cell Structure and Function (in press)
• [Journal Article] TNFα-induced ATF3 Expression Is Bidirectionally Regulated by the JNK and ERK Pathways in Vascular Endothelial Cells (2004)
  • Author(s): Inoue, K., Zama, T., Kamimoto, T., Aoki, R., Ikeda, Y., Kimura, H. & Hagiwara, M.
  • Journal Title: Gene to Cells 1 Pages: 59-70
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Regulation of binding of lamin B receptor to chromatin by SR protein kinase and cdc2 kinase in Xenopus egg extracts (2004)
  • Author(s): Takano, M., Koyama, Y., Ito, H., Hoshino, S., Onogi, H., Hagiwara, M., Furukawa, K., Horigome, T
  • Journal Title: J. Biol. Chem 279 Pages: 13265-13271
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Manipulation of alteRNAtive splicing by a newly developed inhibitor of Clks (2004)
  • Author(s): Muraki, M., Ohkawara, B., Hosoya, T., Onogi, H., Koizumi, J., Koizumi, T., Sumi, K., Yomoda, J., Murray, M. V., Kimura, K., Furuichi, K., Shibuya, H., Krainer, A. R., Suzuki, M., Hagiwara, M
  • Journal Title: J. Biol. Chem 279 Pages: 24246-24254
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Differential expression of glutamate receptors in avian neural pathways for learned vocalization. (2004)
  • Author(s): Wada, K., Sakaguchi, H., Jarvis, E., Hagiwara. M
  • Journal Title: J. Comp. Neurol. 476 Pages: 44-64
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Differential expression of glutamate receptors in avian neural pathways for learned vocalization. (2004)
  • Author(s): Wada, K., Sakaguchi, H., Jarvis, E., Hagiwara, M.
  • Journal Title: J. Comp. Neurol. 476 Pages: 44-64
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Manipulation of alternative splicing by a newly developed inhibitor of Clks. (2004)
  • Author(s): Muraki, M., Ohkawara, B., Hosoya, T., Onogi, H., Koizumi, J., Koizumi, T., Sumi, K., Yomoda, J., Murray, M. V., Kimura, K., Furuichi, K., Shibuya, H., Krainer, A. R., Suzuki, M. Hagiwara, M.
  • Journal Title: J. Biol. Chem. 279 Pages: 24246-24254
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Regulation of binding of lamin B receptor to chromatin by SR protein kinase and cdc2 kinase in Xenopus egg extracts. (2004)
  • Author(s): Takano, M., Koyama, Y., Ito, H., Hoshino, S., Onogi, H., Hagiwara, M., Furukawa, K., Horigome, T
  • Journal Title: J. Biol. Chem. 279 Pages: 13265-13271
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] TNFα-induced ATF3 Expression Is Bidirectionally Regulated by the JNK and ERK Pathways in Vascular Endothelial Cells. (2004)
  • Author(s): Inoue, K., Zama, T., Kamimoto, T., Aoki, R., Ikeda, Y., Kimura, H., Hagiwara, M.
  • Journal Title: Gene to Cells. 1 Pages: 59-70
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] TNFα-induced ATF3 Expression Is Bidirectionally Regulated by the JNK and ERK Pathways in Vascular Endothelial Cells. (2004)
  • Author(s): Inoue, K., Zama, T., Kamimoto, T., Aoki, R., Ikeda, Y., Kimura, H., Hagiwara, M.
  • Journal Title: Gene to Cells 1 Pages: 59-70
• [Journal Article] Regulation of binding of lamin B receptor to chromatin by SR protein kinase and cdc2 kinase in Xenopus egg extracts. (2004)
  • Author(s): Takano, M., Koyama, Y., Ito, H., Hoshino, S., Onogi, H., Hagiwara, M.
  • Journal Title: J.Biol.Chem. 279 Pages: 13265-13271
• [Journal Article] Manipulation of alternative splicing by a newly developed inhibitor of Clks. (2004)
  • Author(s): Muraki, M., Ohkawara, B., Hosoya, T., Onogi, H., Koizumi, J., Koizumi, T., Sumi, K., Yomoda, J., Murray, M.V., Kimura, K., Furuichi, K., Shibuya, H., Krainer, A.R., Suzuki, M., Hagiwara, M.
  • Journal Title: J.Biol.Chem. 279 Pages: 24246-24254
• [Journal Article] Differential expression of glutamate receptors in avian neural pathways for learned vocalization. (2004)
  • Author(s): Wada, K., Sakaguchi, H., Jarvis, E., Hagiwara, M.
  • Journal Title: J.Comp.Neurol. 476 Pages: 44-64
• [Journal Article] The fission yeast ptr1+ gene involved in nuclear mRNA export encodes a putative ubiquitin ligase (2004)
  • Author(s): Tomoko Andoh, Abul Kalam Azad, Asako Shigematsu, Yasumi Ohshima, Tokio Tani
  • Journal Title: Biochem.Biophys.Res.Commun 317 Pages: 1138-1143
• [Journal Article] The nucleolus is involved in mRNA export from the nucleus in fission yeast. (2004)
  • Author(s): Takashi Ideue, Abul Kalam Azad, Jun-ichi Yoshida, Tadao Matsusaka, Mitsuhiro Yanagida, Yasumi Ohshima, Tokio Tani
  • Journal Title: J.Cell Sci 117 Pages: 2887-2895
• [Journal Article] Novel SR-related protein Clasp specifically interacts with inactivated Clk4 and induces the exon EB inclusion of Clk. (2003)
  • Author(s): Katsu, R., Onogi, H., Wada, K., Kawaguchi, K., & Hagiwara. M
  • Journal Title: J. Biol. Chem 277 Pages: 44220-44228
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Involvement of MKK6 in TCRa beta(int)CD69(lo) : a target population forapoptotic cell death in thymocytes (2003)
  • Author(s): Suzuki, H., Wu, JH, Hossain, K., Ohhata, T., Du, J., Akhand, AA., Hayakawa, A., Kimura, H., Hagiwara, M., Nakashima, I
  • Journal Title: FASEB J 17 Pages: 1538-40
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Effect of cisplatin treatment on speckled distribution of a serine/arginine-rich nuclear proteinCROP/Luc7A (2003)
  • Author(s): Umehara, H., Nishii, Y., Morishima, M., Kakehi, Y., Kioka, N., Amachi, T., Koizumi, J., Hagiwara, M. & Ueda, K
  • Journal Title: Biochem. Biophys. Res. Comm. 301 Pages: 324-329
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Involvement of MKK6 in TCRa beta(int)CD69(lo) : a target population for apoptotic cell death in thymocytes. (2003)
  • Author(s): Suzuki, H., Wu, JH, Hossain, K., Ohhata, T., Du, J., Akhand, AA., Hayakawa, A., Kimura, H., Hagiwara, M., Nakashima, I.
  • Journal Title: FASEB J. 1538 Pages: 17-40
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A novel dual-specificity phosphatase SKRP1 interacts with the MAPKKMKK7 and inactivates the JNK MAPK pathway : Implication for the precise regulation of the particular MAPK pathway (2002)
  • Author(s): Zama, Z., Aoki, R., Kamimoto, T., Inoue, K., Ikeda, Y. & Hagiwara M.
  • Journal Title: J. Biol. Chem 277 Pages: 23909-23918
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Scaffold role of a MAP linase phosphatase, SKRPl for the JNK signaling pathway・ (2002)
  • Author(s): Zama, Z., Aoki, R., Kamimoto, T., Inoue, K., Ikeda, Y. & Hagiwara M.
  • Journal Title: J. Biol. Chem 277 Pages: 23919-23926
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Identification of methylated protein arginine N-methyltransferase 1, PRMT1 with a new express ion cloning strategy (2002)
  • Author(s): Wada, K., Inoue, K, & Hagiwara. M.
  • Journal Title: Biochem. Biophys. Acta. 1591 Pages: 1-10
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] CaMK cascade activates CRE-mediated transcription in neurons of Caenorhabditis elegans (2002)
  • Author(s): Kimura, Y., Corcoran, E. E., Eto K., Gengyo-Ando, K., Muramatsu, M., Kobayashi, R., Freedman, J H., Mitani, S., Hagiwara. M., Means, A. R., & Tokumitsu H
  • Journal Title: EMBO Rep 3 Pages: 962-966
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Identification of ATF-2 as a Transcriptional Regulator for the Tyrosine Hydroxylase Gene (2002)
  • Author(s): Suzuki, T., Yamakuni, T., Hagiwara. M., & Ichinose, H
  • Journal Title: J. Biol. Chem 277 Pages: 40768-40774
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] M-kinase cascade activates CRE-mediated transcription Identification of ATF2. (2002)
  • Author(s): Suzuki, T., Yamakuni, T., Hagiwara, M., Ichinose, H.
  • Journal Title: J. Biol. Chem. (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] M-kinase cascade activates CRE-mediated transcription in neurons of Caenorhabditis elegans (2002)
  • Author(s): Kimura, Y., Corcoran, E. E., Eto K., Gengyo-Ando, K., Muramatsu, M., Kobayashi, R., Freedman, J. H., Mitani, S., Hagiwara, M., Means, A. R., Tokumitsu H.
  • Journal Title: EMBO Rep. (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Identification of methylated protein arginine N-methyltransferase 1, PRMT1 with a new expression cloning strategy. (2002)
  • Author(s): Wada, K., Inoue, K., Hagiwara, M.
  • Journal Title: Biochem. Biophys. Acta. 1591 Pages: 1-10
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A novel dual-specificity phosphatase SKRP1 interacts with the MAPKK MKK7 and inactivates the JNK MAPK pathway : Implication for the precise regulation of the particular MAPK pathway. (2002)
  • Author(s): Zama, Z., Aoki, R., Kamimoto, T., Inoue, K., Ikeda, Y., Hagiwara M.
  • Journal Title: J. Biol. Chem. 277 277 Pages: 23909-23918
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Scaffold role of a MAP linase phosphatase, SKRP1 for the JNK signaling pathway. (2002)
  • Author(s): Zama, Z., Aoki, R., Kamimoto, T., Inoue, K., Ikeda, Y., Hagiwara M.
  • Journal Title: J. Biol. Chem. 277 Pages: 23919-23926
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Novel SR-related protein Clasp specifically interacts with inactivated Clk4 and induces the exon EB inclusion of Clk. (2002)
  • Author(s): Katsu, R., Onogi, H., Wada, K., Kawaguchi, K., Hagiwara, M.
  • Journal Title: J. Biol. Chem. 277 Pages: 44220-44228
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Identification of methylated protein arginine N-methyltransferase 1, PRMT1 with a new expression cloning strategy. (2002)
  • Author(s): Kazuhiro, W, Koichi, I, Hagiwara, M.
  • Journal Title: Biochem. Biophys. Acta. 1591 Pages: 1-10
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Visualization of developmentally regulated alternative splicing events by a GFP/RFP based splicing reporter system (2007)
  • Author(s): 萩原 正敏
  • Organizer: 07'遺伝情報DECODE・冬のワークショップ
  • Place of Presentation: 湯沢グランドホテル
  • Year and Date: 2007-01-27
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 記録なし (2007)
  • Author(s): 萩原 正敏
  • Organizer: Functional Organization of the Nucleus
  • Place of Presentation: 淡路夢舞台国際会議場
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Cap-binding complex recrits REF to mRNA in a splicing-independent manner for mRNA export (2007)
  • Author(s): Hagiwara M
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] mRNA核外輸送に関与する分裂酵母ptr5^+遺伝子は核膜孔複合体因子Nup85pをコードする (2006)
  • Author(s): 渡邉常義
  • Organizer: 日本分子生物学会2006フォーラム
  • Place of Presentation: 名古屋
  • Year and Date: 2006-12-07
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 出芽酵母新規bud-tip局在化RNA YOR066wの局在化機構と細胞周期における機能解析 (2006)
  • Author(s): 大城夕希子
  • Organizer: 日本分子生物学会2006フォーラム
  • Place of Presentation: 名古屋
  • Year and Date: 2006-12-07
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] SRPKl阻害剤によるリバースケミカルジェネティクス (2006)
  • Author(s): 萩原 正敏
  • Organizer: 第8回RNAミーティング
  • Place of Presentation: 淡路夢舞台国際会議場
  • Year and Date: 2006-07-20
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Alternative Splicing Reporter Worms Reveal Cell-Type-Specifiv Expression Profiles and Regulation Mechanism in Vivo (2006)
  • Author(s): 黒柳 秀人
  • Organizer: 第8回RNAミーティング
  • Place of Presentation: 淡路夢舞台国際会議場
  • Year and Date: 2006-07-20
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 新規のlgCAM細胞接着因子MDGAlへ大脳皮質表層への神経細胞の移動と層形成を制御する (2006)
  • Author(s): 武内 章英
  • Organizer: 第29回日本神経科学大会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2006-07-20
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] mRNA核外輸送に関与する膜結合型因子PtrlOpの細胞内ダイナミクス (2006)
  • Author(s): 松下桐子
  • Organizer: 第8回日本RNA学会年会
  • Place of Presentation: 淡路市
  • Year and Date: 2006-07-19
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 出芽酵母を用いた細胞内局在化RNAの網羅的探索と解析 (2006)
  • Author(s): 安東知子
  • Organizer: 第8回日本RNA学会年会
  • Place of Presentation: 淡路市
  • Year and Date: 2006-07-19
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] A transpenic reporter reveals cell-type-specific expression profiles and regulation mechanisms of mutually-exelusive alternative exons in vivo (2006)
  • Author(s): 黒柳 秀人
  • Organizer: 第20回国際生化学・分子生物学会議
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2006-06-19
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] U4核内低分子RNAの変異によるイントロン選択的スプライシング阻害 (2006)
  • Author(s): 知念まどか
  • Organizer: 第6回細胞核ダイナミクス研究会
  • Place of Presentation: 熊本
  • Year and Date: 2006-05-17
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Reverse chemical genetics による選択的スプライシング制御機構の解明 (2006)
  • Author(s): 萩原 正敏
  • Organizer: 日本科学会第86回春季年会
  • Place of Presentation: 日本大学理工学部
  • Year and Date: 2006-03-27
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 創薬の未来を開くケミカルバイオロジー (2006)
  • Author(s): 萩原 正敏
  • Organizer: 第79回日本薬理学会年会
  • Place of Presentation: パシフイコ横浜
  • Year and Date: 2006-03-09
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] RNA結合蛋白を標的とする新規抗ウィルス薬開発 (2006)
  • Author(s): 萩原 正敏
  • Organizer: 大学発新技術説明会
  • Place of Presentation: 大阪科学技術センター
  • Year and Date: 2006-02-24
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] タンパクリン酸化酵素阻害剤の創薬可能性について (2006)
  • Author(s): 萩原 正敏
  • Organizer: 第2回ケミカルバイオロジーフロンティア国際シンポジウム
  • Place of Presentation: 東京医科歯科大学
  • Year and Date: 2006-02-17
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Reverse chemical genetics with synthetic protein kinase inhibitors (2006)
  • Author(s): 萩原 正敏
  • Organizer: 日本化学会
  • Place of Presentation: 東京
  • Year and Date: 2006-01-27
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 選択的スプライシングのリン酸化制御機構 (2006)
  • Author(s): 萩原 正敏
  • Organizer: 生物新機能と創薬をめざす生体内分子科学第8回シンポジウム
  • Place of Presentation: ぱるるプラザ岐阜
  • Year and Date: 2006-01-13
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] CaM-KカスケードおよびCREBはC. elegansの耐性幼虫形成を制御する (2006)
  • Author(s): 川俣 光太郎
  • Organizer: 遺伝情報DECODE・冬のワークショップ
  • Place of Presentation: 湯沢ニューオータニホテル
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 新規in vitro 転写/スプライシング共役系を用いた解析 : mRNAへの輸送アダプターAly/REFのRNAPII依存的結合促進 (2006)
  • Author(s): 野島 孝之
  • Organizer: 遺伝情報DECODE-冬のワークショップ
  • Place of Presentation: 湯沢ニューオータニホテル
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Manipulation of mRNA splicing with Protein Kinasa Inhibitors (2006)
  • Author(s): 萩原 正敏
  • Organizer: 第20回国際生化学・分子生物学会議
  • Place of Presentation: 国立京都国際会館
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Visualization of tissue-specific alternative splicing and cloning of the regulatory proteins in C. elegans (2006)
  • Author(s): 萩原 正敏
  • Organizer: 2006 GRC on Post・Transcriptional Gene regulation, the Biology of
  • Place of Presentation: オックスフォード大学
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] RNAPII transcription enhanced recruitment of an mRNA export adaptor, Aly/REF under in vitro transcription/splicing coupling condition. (2006)
  • Author(s): 野島 孝之
  • Organizer: 第20回国際生化学・分子生物学会議
  • Place of Presentation: 国立京都国際会館
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] ウィルスRNAを標的とするSRPK阻害剤SRPIN340の創薬 (2006)
  • Author(s): 萩原 正敏
  • Organizer: 日本ウィルス学会第54回学術集合プログラム
  • Place of Presentation: 名古屋国際会議場
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 選択的スプライシングを生体内で可視化する (2006)
  • Author(s): 黒柳 秀人
  • Organizer: 第309回生物科学セミナー
  • Place of Presentation: 岡山大学
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 選択的スプライシングパターンを生体内で可視化する (2006)
  • Author(s): 黒柳 秀人
  • Organizer: 第2回神戸スプライシングカンファレンス
  • Place of Presentation: 神戸大学
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Transgenic alternative -splicing reporters reveal tissue-specific expressionprofiles and regulation mechanism in vivo (2006)
  • Author(s): 黒柳 秀人
  • Organizer: CDBセミナー
  • Place of Presentation: 理化学研究所神戸
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] ケミカルジェネテイクスによるドグマへの挑戦 (2006)
  • Author(s): 萩原 正敏
  • Organizer: 第80回日本薬理学会年会
  • Place of Presentation: 名古屋国際会議場
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Visualization of tissue-specific alternative splicing and cloning of the regulatory proteins in C. elegans (2006)
  • Author(s): Hidehito Kuroyanagi, Masatoshi Hagiwara
  • Organizer: Gordon Research Conferences
  • Place of Presentation: POST-TRANSCRIPTIONAL GENE REGULATION, THE BIOLOGY OF, Oxford, United Kingdom
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Protein kinase inhibitors and their clinical potential (2006)
  • Author(s): Hagiwara M
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Alternative splicing a new drug target of the post-genome era (2006)
  • Author(s): Hagiwara M
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Molecular manipulation of alternative splicing (2006)
  • Author(s): Hagiwara M
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Visualizaition of tissue-specific alternative splicing and cloning of the regulatory proteins in C. elegans (2006)
  • Author(s): Hagiwara M
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Visualization and manipulation of the tissue-specific alternative splicing of mRNA (2006)
  • Author(s): Hagiwara M
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 細胞周期異常を示す分裂酵母pre-mRNAスプライシング変異株prpl3の解析 (2005)
  • Author(s): 知念まどか
  • Organizer: 第28回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2005-12-08
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] mRNAの核外輸送とユビキチン・プロテアソーム機構に関与する多機能因子Dsslの解析 (2005)
  • Author(s): 萬年太郎
  • Organizer: 第28回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2005-12-08
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 出芽酵母における新規細胞内局在化RNAの解析 (2005)
  • Author(s): 大城夕希子
  • Organizer: 第28回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2005-12-08
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 多細胞生物の多様性を生み出した選択的スプライシングの制御機構 (2005)
  • Author(s): 黒柳 秀人
  • Organizer: 日本生物物理学会第43回年会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2005-11-24
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 特異的リン酸化酵素阻害剤によるリバースケミカルジェネティクスの展開 (2005)
  • Author(s): 萩原 正敏
  • Organizer: 第3回ケミカルバイオロジーシンポジウム「ケミカルバイオロジー研究の新展開
  • Place of Presentation: 学術総合センター一ツ橋記念講堂
  • Year and Date: 2005-11-09
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 出芽酵母における細胞内局在化RNAの網羅的スクリーニング (2005)
  • Author(s): 安東知子
  • Organizer: 第78回日本生化学学会
  • Place of Presentation: 神戸
  • Year and Date: 2005-10-19
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] イントロン結合タンパク質X160のEJC会合における役割 (2005)
  • Author(s): 井手上 賢
  • Organizer: 第7回日本RNA学会年会
  • Place of Presentation: 広前大学
  • Year and Date: 2005-08-11
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 記録なし (2005)
  • Author(s): 萩原 正敏
  • Organizer: 第11回成人病の病因・病態の解明に関する研究会
  • Place of Presentation: 軽井沢プリンスホテル
  • Year and Date: 2005-07-10
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Manipulation of alternative splicing by specific inhibitors of SR proteinkinases (2005)
  • Author(s): 萩原 正敏
  • Organizer: 4th International Conference on Inhibitors of Protein Kinases
  • Place of Presentation: ワルシャワ大学カンファレンスサイト グダニスク大学
  • Year and Date: 2005-06-29
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] CaM-K cascade and CREB regulate C. elegans dauer formation (2005)
  • Author(s): 黒柳 秀人
  • Organizer: 15^<th> International C. elegans Meeting
  • Place of Presentation: UCLA大学
  • Year and Date: 2005-06-28
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] CaM-KカスケードおよびCREBはC. elegansの耐性幼虫形成を制御する (2005)
  • Author(s): 黒柳 秀人
  • Organizer: 第28回日本神経科学大会
  • Place of Presentation: パシフイコ横浜
  • Year and Date: 2005-06-27
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] CREB研究のOverview・モデル生物を用いたCREB研究 (2005)
  • Author(s): 萩原 正敏
  • Organizer: CREB-FORUM
  • Place of Presentation: 京王プラザホテル47階あさひ
  • Year and Date: 2005-05-21
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 記録なし (2005)
  • Author(s): 萩原 正敏
  • Organizer: RNA情報網・第3回合同班会議
  • Place of Presentation: 東レ総合研修センター
  • Year and Date: 2005-01-06
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] How to arrest high-jacking of human gene expression system by virus. (2005)
  • Author(s): 萩原 正敏
  • Organizer: JBSバイオシンポジウム「転写研究の新しいフロンティア」
  • Place of Presentation: 群馬県草津町
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Signal-dependent regulation of SR proteins and its effect on HIV replication (2005)
  • Author(s): 福原 武志
  • Organizer: RNA2005 : Tenth Annual Meeting of the RNA SOCIETY
  • Place of Presentation: カナダ山岳国立公園
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 記録なし (2005)
  • Author(s): 萩原 正敏
  • Organizer: EUKARYOTIC mRNA PROCESSING
  • Place of Presentation: CSHL
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] CaM-KカスケードおよびCREBはC. elegansの耐性幼虫形成を制御する (2005)
  • Author(s): 黒柳 秀人
  • Organizer: 第28回日本分子生物学会年会
  • Place of Presentation: 福岡Yahoo! JAPANドーム
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Manipulation of alternative splicing by specific inhibitors of SR protein kinases. 4th International Conference on Inhibitors of Protein Kinases (IPK 2005) (2005)
  • Author(s): Hagiwara M.
  • Place of Presentation: Warsaw, Poland
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Mechanistic insights into the linkage between pre-mRNA splicing and snoRNP biogenesis. (2005)
  • Author(s): Hirose T.
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Signal-dependent regulation of SR proteins and its effect on HIV replication. Tenth Annual Meeting of the RNA Society (RNA 2005) (2005)
  • Author(s): Fukuhara T., Hosoya T., Shimizu S. Suzuki M., Yamamoto N., Hagiwara M.
  • Place of Presentation: Banff, Alberta, Canada
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] CaM-K cascade and CREB regulate C. elegans dauer formation. 15th International C. (2005)
  • Author(s): KUROYANAGI H., YOSHIMURA S., KIMURA Y., HAGIWARA M.
  • Organizer: elegans Conference
  • Place of Presentation: Los Angeles, CA, USA
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] DEVELOPMENT OF A COUPLED IN VITRO TRANSCRIPTION-SPLICING SYSTEM FROM HELA CELL NUCLEAR EXTRACT (2005)
  • Author(s): Takayuki Nojima, Tetsuro Hirose, Masatoshi Hagiwara.
  • Organizer: Cold Spring Harbor Laboratory Meeting 2005, Eukaryotic mRNA Processing.
  • Place of Presentation: Cold Spring Harbor, NY, USA.
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] How to arrest high-jacking of human gene expression system by virus. (2005)
  • Author(s): bHagiwara M.
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 哺乳類のsnoRNP 生合成に関わる複数の核内因子 (2004)
  • Author(s): 廣瀬 哲郎
  • Organizer: 第27回日本分子生物学会年会
  • Place of Presentation: 神戸国際展示場
  • Year and Date: 2004-12-19
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 異常スプライシングに起因する疾患の克服に向けて (2004)
  • Author(s): 萩原 正敏
  • Organizer: 第27回日本分子生物学会年会
  • Place of Presentation: ポートピアホテル
  • Year and Date: 2004-12-09
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 分裂酵母U2AF複合体はordered exon joiningを制御する (2004)
  • Author(s): 原口典子
  • Organizer: 第27回日本分子生物学会
  • Place of Presentation: 神戸
  • Year and Date: 2004-12-08
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 分裂酵母におけるmRNA核外輸送変異株ptr8-lのマルチコピーサプレッサー遺伝子の単離と解析 (2004)
  • Author(s): 栗原幹子
  • Organizer: 第27回日本分子生物学会
  • Place of Presentation: 神戸
  • Year and Date: 2004-12-08
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Signal dependent regulation of SR proteins and its effect on HIV replication (2004)
  • Author(s): 福原 武志
  • Organizer: 第4回あわじしま感染症・免疫フォーラム
  • Place of Presentation: 淡路夢舞台国際会議場
  • Year and Date: 2004-08-31
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] TG003, specific of Clkl/Sty affects the distribution of nuclear speckles and alternative splicing of mRNAs (2004)
  • Author(s): 萩原 正敏
  • Organizer: 第16回国際解剖学会議
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2004-08-24
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 分裂酵母を用いたordered exon joiningに関与する因子の同定 (2004)
  • Author(s): 原口典子
  • Organizer: 第6回RNAミーティング
  • Place of Presentation: 熊本
  • Year and Date: 2004-08-06
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 分裂酵母におけるmRNA 核外輸送に関与するptrl変異遺伝子のサプレッサーの単離と解析 (2004)
  • Author(s): 坂口奈央子
  • Organizer: 第6回RNAミーティング
  • Place of Presentation: 熊本
  • Year and Date: 2004-08-06
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 出芽酵母Saccharomyces cerevisiae におけるストレス応答mRNA選択的核外輸送関連遺伝子のゲノムワイドスクリーニング (2004)
  • Author(s): 楠田文
  • Organizer: 第6回RNAミーティング
  • Place of Presentation: 熊本
  • Year and Date: 2004-08-04
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] SR protein kinasa, SPK-l, regulates multiple aspect of post-fertilization development in C (2004)
  • Author(s): 黒柳 秀人
  • Organizer: East Asia C, elegans Meeting Program
  • Place of Presentation: 淡路夢舞台国際会議場
  • Year and Date: 2004-06-30
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Dauer Regulatory Functions of CaMK-CREB cascade (2004)
  • Author(s): 吉村 祥子
  • Organizer: East Asia C, elegans Meeting Program
  • Place of Presentation: 淡路夢舞台国際会議場
  • Year and Date: 2004-06-30
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 選択的スプライシング : ポストゲノム時代の新しい創薬ターゲット (2004)
  • Author(s): 萩原 正敏
  • Organizer: 生物新機能と創薬をめざす生体内分子科学
  • Place of Presentation: 北海道大学
  • Year and Date: 2004-06-29
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Dauer Regulatory Functions of CaMK - CREB cascade. (2004)
  • Author(s): S. Yoshimura, H. Kuroyanagi, Y. Kimura, M. Hagiwara.
  • Place of Presentation: 2004 East Asia Worm Meeting
  • Year and Date: 2004-06-07
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] SR protein kinese, SPK-1, regulates multiple aspect of post-fertilization development in C.elegans (2004)
  • Author(s): H. Kuroyanagi, M. Hagiwara.
  • Place of Presentation: 2004 East Asia Worm Meeting
  • Year and Date: 2004-06-07
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] イントロから生成される核小体低分子RNPの機能と生合成機構 (2004)
  • Author(s): 廣瀬 哲郎
  • Organizer: 特定領域研究「RNA情報網」第2回合同班会議
  • Place of Presentation: マリンヒルホテル小樽
  • Year and Date: 2004-02-02
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 線虫CREBの標的遺伝子の探索 : プロテオームVScDNAマイクロアレイVS In Silico (2004)
  • Author(s): 萩原 正敏
  • Organizer: 第3回転写研究会
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2004-01-17
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] MRNAをターゲットとする新しい抗ウィルス薬の可能性について (2004)
  • Author(s): 萩原 正敏
  • Organizer: 「生物新機能と創薬をめざす生体内分子科学」第5回シンポジウム
  • Place of Presentation: ぱるるプラザ岐阜
  • Year and Date: 2004-01-07
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 記録なし (2004)
  • Author(s): 萩原 正敏
  • Organizer: Dynamic Organization of Nuclear Function ミーティング
  • Place of Presentation: CSHL
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Chemical Modulators of Alternative Splicing and Their Clinical Application. (2004)
  • Author(s): Hagiwara M.
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Chemical Modulators of Alternative Splicing and Their Clinical Application. (2004)
  • Author(s): Hagiwara M.
  • Organizer: Hirose T. Novel Coupling Mechanism Between Splicing and Intron-Encoded snoRNP Biogenesis. 2. JBS
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 蛍光イメージングによるmRNA核外輸送機構の解析 : スプライシング反応はmRNA核外輸送を促進する (2003)
  • Author(s): 徳永和明
  • Organizer: 第26回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2003-12-10
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 分裂酵母における核小体依存的mRNA核外輸送 (2003)
  • Author(s): 井手上賢
  • Organizer: 第26回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2003-12-10
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Split hand/split foot diseaseの原因遺伝子DSSlはmRNAの核外輸送に関与する (2003)
  • Author(s): 萬年太郎
  • Organizer: 第26回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2003-12-10
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 記録なし (2003)
  • Author(s): 萩原 正敏
  • Organizer: 特定領域研究「RNA情報網」班会議
  • Place of Presentation: 神戸大学
  • Year and Date: 2003-09-04
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 高等真核生物生細胞におけるクロマチン転写機構の解析にむけて (2003)
  • Author(s): 木村 宏
  • Organizer: 先端領域融合医学研究機構開設記念シンポジウム
  • Place of Presentation: 京都大学
  • Year and Date: 2003-05-21
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 生細胞クロマチンのカイネティクス : H2Aバリアントの役割とヒストン分子の交換を制御する因子の探索 (2003)
  • Author(s): 木村 宏
  • Organizer: 第56回日本細胞生物学会大会
  • Place of Presentation: ピアザ淡海滋賀県立県民交流センター
  • Year and Date: 2003-05-14
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 新規ベンゾチアソール化合物による選択的スプライシングの制御 (2003)
  • Author(s): 村木 倫子
  • Organizer: 第76回日本薬理学会年会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2003-03-24
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] ヒストンH2Aバリアントの生細胞核内における局在と流動性 (2003)
  • Author(s): 木村 宏
  • Organizer: 第20回染色体ワークショップ
  • Place of Presentation: ウェルサンピア京都
  • Year and Date: 2003-02-01
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 線虫CREB標的遺伝子のプロテオミクス解析 (2003)
  • Author(s): 萩原 正敏
  • Organizer: 第2回転写研究会
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2003-01-24
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] スプライシング異常を伴う疾患群と薬剤による選択的スプライシングの制御 (2003)
  • Author(s): 萩原 正敏
  • Organizer: 生物新機能と創薬をめざす生体内分子科学第4回シンポジウム
  • Place of Presentation: メルパルク名古屋
  • Year and Date: 2003-01-08
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Manipulation of alternative splicing by novel kinase inhibitors (2003)
  • Author(s): 萩原 正敏
  • Organizer: Opening lecture of the 39^<th> Meeting of the Polish Biochemical Society
  • Place of Presentation: Gdansk, Poland
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Understanding the role of RNA processing related PRP4 kinase by identification of its binding proteins (2003)
  • Author(s): Lechward K.
  • Organizer: 39^<th> Meeting of the Polish Biochemical Society
  • Place of Presentation: Gdansk, Poland
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Understanding the role of RNA processing related PRP4 kinase by identification of its binding proteins. (2003)
  • Author(s): Lechward K., Fukuhara T., Hagiwara M.
  • Organizer: 39^<th> Meeting of the Polish Biochemical Society.
  • Place of Presentation: Gdansk, Poland.
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Manipulation of alternative splicing by novel kinase inhibitors. (2003)
  • Author(s): Hagiwara M.
  • Organizer: Opening lecture of the 39^<th> Meeting of the Polish Biochemical Society.
  • Place of Presentation: Gdansk, Poland.
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Dynamic mobility of RNA polymerase II in living cells : implication for the transcription cycle in vivo (2002)
  • Author(s): 木村 宏
  • Organizer: 第25回日本分子生物学会年会
  • Place of Presentation: パシフイコ横浜
  • Year and Date: 2002-12-13
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] ユビキチンリガーゼ変異によるmRNA核外輸送阻害を抑制するsptl遺伝子の解析 (2002)
  • Author(s): 安東知子
  • Organizer: 第25回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2002-12-12
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 線虫におけるCaM-KK/CaM-K/CREB カスケード (2002)
  • Author(s): 木村 芳滋
  • Organizer: 第25回日本分子生物学会年会
  • Place of Presentation: パシフイコ横浜
  • Year and Date: 2002-12-11
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] ブロモウリジンを用いたパルスーチェイス法によるRNAプロセッシング・核外輸送アッセイ系の開発 (2002)
  • Author(s): 四方田 純一郎
  • Organizer: 第25回日本分子生物学会年会
  • Place of Presentation: パシフイコ横浜
  • Year and Date: 2002-12-11
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] スプライシング因子SF2/ASFの過剰発現による転写阻害 (2002)
  • Author(s): 村木 倫子
  • Organizer: 第25回日本分子生物学会年会
  • Place of Presentation: パシフイコ横浜
  • Year and Date: 2002-12-11
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 生GFP融合ヒストンを用いた生細胞におけるヌクレオソーム構造変換のモニタリング (2002)
  • Author(s): 神本 高宏
  • Organizer: 第25回日本分子生物学会年会
  • Place of Presentation: パシフイコ横浜
  • Year and Date: 2002-12-11
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 分裂酵母mRNA核外輸送温度感受性変異株ptrの解析 (2002)
  • Author(s): 井手上賢
  • Organizer: 第25回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2002-12-11
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] pre-mRNAスプライシングとmRNA核外輸送をリンクする分裂酵母Prp3pの機能解析 (2002)
  • Author(s): 石橋まさき
  • Organizer: 第25回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2002-12-11
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] ストレス条件下におけるmRNA核外輸送の制御機構に関する解析 (2002)
  • Author(s): 吉田准一
  • Organizer: 第25回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2002-12-11
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] mRNA核外輸送における基本転写因子複合体TFIIHの役割 (2002)
  • Author(s): 水城史貴
  • Organizer: 第25回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2002-12-11
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 生細胞でのRNAポリメレースIIの動態解析:アクティブな遺伝子は常に転写されているのか? (2002)
  • Author(s): 木村 宏
  • Organizer: 第75回日本生化学会大会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2002-10-17
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] MRNAプロセシングのリン酸化制御機構 (2002)
  • Author(s): 萩原 正敏
  • Organizer: 第75回日本生化学会大会
  • Place of Presentation: 京都宝ヶ池プリンスホテル
  • Year and Date: 2002-10-15
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Signal transduction which regulates mRNA factory. (2002)
  • Author(s): 萩原 正敏
  • Organizer: 山中湖カンフアランス
  • Place of Presentation: 東海大学山中湖セミナーハウス
  • Year and Date: 2002-09-07
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Biobjeによる遺伝子発現シミュレーションの線虫固体への応用 (2002)
  • Author(s): 木村 芳滋
  • Organizer: 特定領域研究『ゲノム』4領域2002年度合同班会議
  • Place of Presentation: 神戸ポートピアホテル
  • Year and Date: 2002-08-21
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Regulation of transcriptional activity of CREB in neurons of c. elegans (2002)
  • Author(s): 木村 芳滋
  • Organizer: 第3回 c. エレガンス日本集会 名古屋
  • Place of Presentation: 名古屋大学
  • Year and Date: 2002-08-06
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 線虫プロテオーム解析実施のための具体的プロ世コール受注システムの詳細について (2002)
  • Author(s): 萩原 正敏
  • Organizer: 特定領域統合ゲノム線虫分科会発足会議
  • Place of Presentation: 東レ総合研修センター
  • Year and Date: 2002-07-21
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] CaM-kinase cascade activates CRE・mediated transcription in neurons of Caenorhabditis elegans (2002)
  • Author(s): 木村 芳滋
  • Organizer: Early 2002 Midwest Worm Meeting
  • Place of Presentation: St. Louis, Missouri
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] A. CaM・kinase cascade activates CRE-mediated transcription in neurons of Caenorhabditis elegans (2002)
  • Author(s): 木村 芳滋
  • Organizer: Gordon Research Conference, Cell Biology of Neuron
  • Place of Presentation: Newport, Rhode Island
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Kinetics of RNA polymerase II in living cells. (2002)
  • Author(s): 木村 宏
  • Organizer: CSHL Dynamic Organization of NuclearFunction
  • Place of Presentation: CSHL
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Kinetics of chromatin proteins in living cells revealed by long-term FRAP (fluorescence recovery after photobleaching). (2002)
  • Author(s): 木村 宏
  • Organizer: Joint A-IMBN/EMBO
  • Place of Presentation: 神戸
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] How to arrest high - jacking of human gene expression system by virus.
  • Author(s): Hagiwara M.
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] SR protein phosphorylation and virus infection.
  • Author(s): Hagiwara M.
  • Description: 「研究成果報告書概要(欧文)」より
• [Patent(Industrial Property Rights)] 生体内における選択的スプライシングレポーター系 (2006)
  • Inventor(s): 萩原正敏, 黒柳秀人
  • Industrial Property Rights Holder: 国立大学法人東京医科歯科大学
  • Filing Date: 2006-09-26
  • Description: 「研究成果報告書概要(和文)」より
  • Overseas
• [Patent(Industrial Property Rights)] 異常スプライシング関連疾患の治療薬候補物質 (2005)
  • Inventor(s): 萩原正敏, 鈴木正昭
  • Industrial Property Rights Holder: 国立大学法人東京医科歯科大学
  • Filing Date: 2005
  • Description: 「研究成果報告書概要(和文)」より
  • Overseas
• [Patent(Industrial Property Rights)] SR蛋白質のリン酸化制御方法、およびSR蛋白質の活性制御剤を有効成分とする抗ウイルス剤 (2003)
  • Inventor(s): 萩原正敏, 福原武志, 鈴木正昭, 細谷孝充
  • Industrial Property Rights Holder: 萩原正敏
  • Industrial Property Number: 2003-435085
  • Filing Date: 2003
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Takano, M, Koyama, Y, Ito, H, Hoshino, S, Onogi, H, Hagiwara, M, Furukawa, K, Horigome, T.: "Regulation of binding of lamin B receptor to chromatin by SR protein kinase and cdc2 kinase in Xenopus egg extracts."J.Biol.Chem.. (in press). (2003)
• [Publications] Inoue, K., Zama, T., Kamimoto, T., Aoki, R., Ikeda, Y., Kimura, H., Hagiwara, M.: "TNFα-induced ATF3 Expression Is Bidirectionally Regulated by the INK and ERK Pathways in Vascular Endothelial Cells."Gene to Cells. (in press). (2003)
• [Publications] Umehara, H., Nishii, Y., Morishima, M., Kakehi, Y., Kioka, N., Amachi, T., Koizumi, J., Hagiwara, M., Ueda, K: "Effect of cisplatin treatment on speckled distribution of a serine/arginine-rich nuclear protein CROP/Luc7A"Biochem.Biophys.Res.Comm.. 301. 324-329 (2003)
• [Publications] Umehara, H., Nishii, Y., Morishima, M., Kakehi, Y., Kioka, N., Amachi, T., Koizumi, J., Hagiwara, M., Ueda, K.: "Effect of cisplatin treatment on speckled distribution of a serine/arginine-rich nuclear protein CROP/Luc7A"Biochem. Biophys. Res. Comm..
• [Publications] Katsu, R., Onogi, H., Wada, K, Kawaguchi, K., Hagiwara, M.: "Novel SR-related protein Clasp specifically interacts with inactivated Clk4 and induces the exon EB inclusion of Clk"J. Biol. Chem.. 277. 44220-44228 (2002)
• [Publications] Suzuki, T., Yamakuni, T., Hagiwara, M., Ichinose, H.: "Identification of ATF-2 as a Transcriptional Regulator for the Tyrosine Hydroxylase Gene"J. Biol. Chem.. 277. 40768-40774 (2002)
• [Publications] Kimura, Y., Corcoran, E.E., Eto K., Gengyo-Ando, K., Muramatsu, M., Kobayashi, R., Freedman, J.H., Mitani, S., Hagiwara, M., Means, A.R., Tokumitsu H.: "A CaMK cascade activates CRE-mediated transcription in neurons of Caenorhabditis elegans"EMBO Rep.. 3. 962-966 (2002)
• [Publications] Kazuhiro, W, Koichi, I, Hagiwara, M: "Identification of methylated protein arginine N-methyltransferase 1, PRMT1 with a new expression cloning strategy"Biochem. Biophys. Acta.. 1591. 1-10 (2002)
• [Publications] Zama, Z., Aoki, R., Kamimoto, T., Inoue, K., Ikeda, Y., Hagiwara M.: "A novel dual-specificity phosphatase SKRP1 interacts with the MAPKK MKK7 and inactivates the JNK MAPK pathway : Implication for the precise regulation of the particular MAPK pathway"J. Biol. Chem.. 277. 23909-23918 (2002)
• [Publications] Zama, Z., Aoki, R., Kamimoto, T., Inoue, K., Ikeda, Y., Hagiwara M.: "Scaffold role of a MAP linase phosphatase, SKRP1 for the JNK signaling pathway"J. Biol. Chem.. 277. 23919-23926 (2002)