Messenger RNA regulation by 5'-cap structure

• Project/Area Number: 14035104
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Kitasato University
• Principal Investigator: MIZUMOTO Kiyohisa Kitasato University, Professor Emeritus, Vice-President (80092344)
• Co-Investigator(Kenkyū-buntansha): INADA Toshifumi Nagoya University, Dpt. ofMol. Biol, Associate professor (40242812)
• Project Period (FY): 2002 – 2006
• Project Status: Completed (Fiscal Year 2006)
• Budget Amount: ¥63,200,000 (Direct Cost: ¥63,200,000); Fiscal Year 2006: ¥11,500,000 (Direct Cost: ¥11,500,000); Fiscal Year 2005: ¥11,500,000 (Direct Cost: ¥11,500,000); Fiscal Year 2004: ¥13,400,000 (Direct Cost: ¥13,400,000); Fiscal Year 2003: ¥13,400,000 (Direct Cost: ¥13,400,000); Fiscal Year 2002: ¥13,400,000 (Direct Cost: ¥13,400,000)
• Keywords: cap structure / RNA polvmerase II / RNA-dependent RNA polymerase / Sendai virus / Influenza virus / mRNA surveillance aystem / translation repression / プロテアソーム / キャッピング / ノンストップmRNA / ポリ(A)鎖 / 転写開始 / mRNA分解制御 / キャッピング酵素 / 転写複合体 / RNA依存RNA合成酵素 / RNAポリメラーゼII(POl II) / pol II-CTD / elF4G / キャップ結合因子(CBC) / RNAポリメラーゼII(pol II) / pol II CTD / 固定化鋳型 / eIF4G

Research Abstract

(A) Enzyme mechanisms of mRNA cap formation in eukaryotes as well as negative-strand RNA viruses have been studied. 1) We have shown that the phosphorylation of RNA polymerase II carboxyl terminal domain (CTD) by TFIIH-associated CTD kinase (CAK) is important for successful capping of nascent RNA chain. Using an in vitro transcription system with immobilized template, we demonstrated that capping occurs when the nascent RNA chain grows to 18-19 nt in length. 2) We demonstrated that SeV RNA-dependent RNA polymerase (RdRP) L protein catalyzes cap methylation of virus specific mRNA. This is to our knowledge the first direct biochemical evidence to show that mononegavirus L protein catalyszes cap G-7-methylation. 3) The influenza virus (FluV) RdRP exhibits a cap-dependent endonuclease activity, which cleaves host mRNAs to produce capped RNA fragments with 11 to13 nucleotides. The resulting capped RNA fragments serve as a primer to initiate viral mRNA synthesis. We found that Flu-A and -B endonucleases exhibited different substrate specificities with regard to the extent as well as the position of cap methylation.
(B) A novel mRNA surveillance for mRNA lacking a termination codon (nonstop mRNA) has been proposed in which Ski7p is thought to recognize stalled ribosomes at the 3' end of mRNA. We found that the level of protein product of nonstop mRNA containing a poly(A) tail was reduced 100-fold, and this reduction was due to rapid mRNA degradation, translation repression and protein destabilization, at least in part, by the proteasome. Insertion of a poly(A) tract upstream of a termination codon resulted in translation repression and protein destabilization but not rapid mRNA decay. We propose that translation of the poly(A) tail plays crucial roles in nonstop mRNA surveillance via translation repression and protein destabilization.

Research Products

• [Journal Article] Translation of poly(A)tail plays crucial roles in nonstop mRNA surveillance viatranslation repression and protein destabilization by proteasome in yeast. (2007)
  • Author(s): Ito-Harashima, S., Kuroha, K., Tatematsu, T., Inada. T
  • Journal Title: Genes Dev. 21 Pages: 519-524
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Translation of poly(A) tail plays crucial roles in nonstop mRNA surveillance via translation repression and protein destabilization by proteasome in yeast. (2007)
  • Author(s): Ito-Harashima, S., Kuroha, K., Tatematsu, T., Inada. T.
  • Journal Title: Genes Dev. 21 Pages: 519-524
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Translation of poly(A) tail plays crucial roles in nonstop. mRNA surveillance via translation reprssion and protein destabilization by proteasome in yeast. (2007)
  • Author(s): Ito-Harashima, S. et al.
  • Journal Title: Genes Dev. 21 (in press)
• [Journal Article] Characterization of monoclonal antibodies directed against the canine distemper virus nucleocapsid protein. (2006)
  • Author(s): Masuda。M., Sato, H., Kamata, H., Katsuo, T., Takenaka, A., Miura。R., Yoneda, M., Tsukiyama-Kohara。K., Mizumoto. K. & Kai C
  • Journal Title: Comp. Immunol. Microbiol. Infect. Dis. 29 Pages: 157-165
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Characterization of monoclonal antibodies directed against the canine distemper virus nucleocapsid protein. (2006)
  • Author(s): Masuda, M, Sato, H., Kamata, H., Katsuo, T., Takenaka, A., Miura, R., Yoneda, M., Tsukiyama-Kohara, K., Mizumoto. K. & Kai, C.
  • Journal Title: Comp. Immunol. Microbiol. Infect. Dis. 29 Pages: 157-165
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Characterization of monoclonal antibodies directed against the canine distemper virus nucleocapsid protein. (2006)
  • Author(s): Masuda, M et al.
  • Journal Title: Comp. Immunol. Microbiol. Infect. Dis. 29 Pages: 157-165
• [Journal Article] 細胞の危機管理システムー細胞を脅かす不良RNAへの対応 (2006)
  • Author(s): 稲田 利文
  • Journal Title: 科学 44 Pages: 589-595
• [Journal Article] 正常と異常を識別する品質管理機構 (2006)
  • Author(s): 稲田 利文
  • Journal Title: 蛋白質・核酸・酵素 51 Pages: 842-843
• [Journal Article] The ARF tumor suppressor inhibits BCL6-mediated transcriptionalrepression. (2005)
  • Author(s): Suzuki, H., Kurita, M., Mizumoto. K., Moriyama, M., Aiso, S., Nishimoto, I., Matsuoka, M.
  • Journal Title: Biochem. Biophys. Res. Commun 326 Pages: 242-248
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Sendai virus RNA-dependent RNA polymerase L protein catalyzes capmethylation of virus specific mRNA. (2005)
  • Author(s): Ogino, T., Kobayashi, M., Iwama, M., Mizumoto. K.
  • Journal Title: J. Biol. Chem、 280 Pages: 4429-4435
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Molecular determinants for subcellular localization of hepatitis C virus core protein. (2005)
  • Author(s): Suzuki, R., Sakamoto, S., Tsutsumi, T., Rikimaru, A., Tanaka, K., Shimoike, T., Moriishi, K., Iwasaki, T., Mizumoto. K., Matsuura, Y, Miyamura, T., Suzuki, T.
  • Journal Title: J. Virol、 79 Pages: 1271-1281
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Translation of aberrant mRNAs lacking a termination codon or with a shortened3'-UTR is repressed after initiation in yeast. (2005)
  • Author(s): Inada, T., Aiba, H.
  • Journal Title: EMBO J. 24 Pages: 1584-1595
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Implication of membrane localization of target mRNA in the action of a small RNA : mechanism of post-transcriptional regulation of glucose transporter in Escherichia coli. (2005)
  • Author(s): Kawamoto, H., Morita, T., Shimizu, A., Inada. T., Aiba, H.
  • Journal Title: Genes Dev. 19 Pages: 328-338
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] The ARF tumor suppressor inhibits BCL6-mediated transcriptional repression. (2005)
  • Author(s): Suzuki, H., Kurita, M., Mizunioto. K., Moriyama, M., Aiso, S., Nishimoto, I. & Matsuoka, M.
  • Journal Title: Biochem. Biophys Res. Commun. 326 Pages: 242-248
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Sendai viras RNA-dependent RNA polymerase L protein catalyzes cap methylation of virus specific mRNA. (2005)
  • Author(s): Ogino, T., Kobayashi, M., Iwama, M. & Mizumoto. K.
  • Journal Title: J. Biol. Chem. 280 Pages: 4429-4435
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Molecular determinants for subcellular localization of hepatitis C virus core protein. (2005)
  • Author(s): Suzuki, R., Sakamoto, S., Tsutsumi, T., Rikimaru, A., Tanaka, K., Shimoike, T., Moriishi, K., Iwasaki, T., Mizumoto. K., Matsuura, Y., Miyamura, T. & Suzuki, T.
  • Journal Title: J. Virol. 79 Pages: 1271-1281
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Translation of aberrant mRNAs lacking a termination codon or with a shortened 3'-UTR is repressed after initiation in yeast. (2005)
  • Author(s): Inada. T., Aiba, H.
  • Journal Title: EMBOJ. 24 Pages: 1584-1595
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Implication of membrane localization of target mRNA in the action of a small RNA: mechanism of post-transcriptional regulation of glucose transporter in Escherichia coli. (2005)
  • Author(s): Kawamoto, H., Morita, T., Shimizu, A., Inada. T., Aiba, H.
  • Journal Title: GenesDev. 19 Pages: 328-338
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Molecular determinants for subcellular localization of hepatitis C virus core protein. (2005)
  • Author(s): R.Suzuki, S.Sakamoto, T.Tsutsum, A.Rikimaru, K.Tanaka, T.Shimoike, K.Moriishi, T.Iwasaki, K.Mizumoto, Y.Matsuura, T.Miyamura, T.Suzuki
  • Journal Title: J.Virol. 79 Pages: 1271-1281
• [Journal Article] Sendaivirus RNA-dependent RNA polymerase L protein catalyzes cap methylation of virus specific mRNA. (2005)
  • Author(s): T.Ogino, M.Kobatashi, M.Iwama, K.Mizumoto
  • Journal Title: J.Biol.Chem. 280 Pages: 4429-4435
• [Journal Article] The ARF tumor suppressor inhibits BCL6-mediated trenscriptional repression. (2005)
  • Author(s): H.Suzuki, M.Kurita, K.Mizumoto, M.Moriyama, S.Aiso, I.Nishimoto, M.Matsuoke
  • Journal Title: Biochem.Biophys.Res.Commun. 326 Pages: 242-248
• [Journal Article] Translation of aberrant mRNA lacking a termination codon or with a shortened 3'-UTR is repressed after initiation in yeast. (2005)
  • Author(s): T.Inada, H.Aiba
  • Journal Title: EMBO J. 24 Pages: 1584-1595
• [Journal Article] Sendai virus RNA-dependent RNA polymerase L protein catalyzes cap methylation of virus specific mRNA. (2005)
  • Author(s): Ogino, T., Kobayashi, M., Iwama, M., Mizumoto, K.
  • Journal Title: J.Biol.Chem. 280 Pages: 4429-4435
• [Journal Article] Molecular determinants for subcellular localization of hepatitis C virus core protein. (2005)
  • Author(s): Suzuki, R., Sakamoto, S., Tsutsumi, T., Rikimaru, A, Tanaka, K., Shimoike, T., Moriishi, K., Iwasaki, T., Mizumoto, K., Masuura, Y., Miyamura, T., Susuki, T.
  • Journal Title: J.Virol. 79 Pages: 1271-1281
• [Journal Article] The ARF tumor suppressor inhibits BCL6-mediated transcriptional repression. (2005)
  • Author(s): Suzuki, H., Kurita, M., Mizumoto, K., Moriyama, M., Aiso, S., Nishimoto, I., Matsuoka, M.
  • Journal Title: Biochem.Biophys.Res.Commun. 326 Pages: 242-248
• [Journal Article] Translation of aberrant mRNAs lacking a termination codon or with a shortened 3'-UTR is repressend after initiation in yeast. (2005)
  • Author(s): Inada, T., Aiba, H.
  • Journal Title: EMBOJ. (in press)
• [Journal Article] Implication of membrane localization of target mRNA in the action of a small RNA : mechanism of post-transcriptional regulation of glucose transporter in Escherichia coll. (2005)
  • Author(s): Kawamoto, H., Morita, T., Shimizu, A., Inada, T., Aiba, H.
  • Journal Title: Genes Dev. 19 Pages: 328-338
• [Journal Article] Overexpression of CR/periphilin downregulates Cdc7 expression and inducesS-phase arrest. (2004)
  • Author(s): Kurita. M., Suzuki, H., Masai, H., Mizumoto. K.. Ogata, E., Nishimoto, I., Aiso, S., Matsuoka, M.
  • Journal Title: Biochem. Biophys. Res. Commun. 324 Pages: 554-561
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Post-transcriptional regulation of HO expression by the Mktl-Pbpl complex. (2004)
  • Author(s): Tadauchi, T., Inada. T. Matsumoto, K., Irie, K.
  • Journal Title: Mol. Cell Biol. 24 Pages: 3670-3681
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Ribosome stalling during translation elongation induces cleavage of mRNA beingtranslated in Escherichia coli. (2004)
  • Author(s): Sunohara, T., Jojima, K., Tagami, H., Inada. T., Aiba, H.
  • Journal Title: J. Biol. Chem 279 Pages: 15368-15375
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Nascent peptide-mediated ribosome stalling at stop codons induces mRNA cleavagesresulting in nonstop mRNAs that are recognized by tmRNA. (2004)
  • Author(s): Sunohara, T., Jojima, K., Yamamoto, Y., Inada. T., Aiba. H.
  • Journal Title: RNA 10 Pages: 378-386
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Overexpression of CR/periphilin downregulates Cdc7 expression and in duces S-phase arrest. (2004)
  • Author(s): Kurita, M., Suzuki, H., Masai, H., Mizumoto, K., Ogata, E., Nishimoto, I., Aiso, S. & Matsuoka, M.
  • Journal Title: Biochem. Bwphys. Res. Commun. 324 Pages: 554-561
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Post-transcriptional regulation of HO expression by the Mktl-Pbpl complex. (2004)
  • Author(s): Tadauchi, T., Inada. T., Matsumoto, K., Irie, K.
  • Journal Title: Mol. Cell Biol. 24 Pages: 3670-3681
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Ribosome stalling during translation elongation induces cleavage of mRNA being translated in Eschenchia coli. (2004)
  • Author(s): Sunohara, T., Jojima, K., Tagami, H., Inada. T., Aiba, H.
  • Journal Title: J. Biol. Chem. 279 Pages: 15368-15375
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Nascent peptidemediated ribosome stalling at stop codons induces mRNA cleavages resulting in nonstop mRNAs that are recognized by tmRNA. (2004)
  • Author(s): Sunohara, T., Jojima, K., Yamamoto, Y., Inada, T., Aiba, H.
  • Journal Title: RNA 10 Pages: 378-386
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Post-transcriptional regulation of HO expression by the Mkt1-Pbp1 complex. (2004)
  • Author(s): Tadauchi, T., Inada, T., Matsumoto, K., Irie, K.
  • Journal Title: Mol.Cell Biol. 24 Pages: 3670-3681
• [Journal Article] Ribosome stalling during translation elongation induces cleavage of mRNA being translated in Escherichia coil. (2004)
  • Author(s): Sunohara, T., Jojima, K., Tagami, H., Inada, T., Aiba, H.
  • Journal Title: J.Biol.Chem. 279 Pages: 15368-15375
• [Journal Article] Nascent peptide-mediated ribosome stalling at stop codons induces mRNA cleavages resulting in nonstop mRNAs that are recognized by tmRNA. (2004)
  • Author(s): Sunohara, T., Jojima, K., Yamamoto, Y., Inada, T., Aiba, H.
  • Journal Title: RNA 10 Pages: 378-386
• [Journal Article] Enolase in the RNA degradosome plays a crucial role in the rapid decay of glucose transporter mRNA in the response to phosphosugar stress in Eschericha coil. (2004)
  • Author(s): Morita, T., Kawamoto, H., Mizota, T., Inada, T., Aiba, H.
  • Journal Title: Mol.Microbiol. 54 Pages: 1063-1075
• [Journal Article] Metabolic block at early stages of glycolytic pathway leads to the activation RcsC/B system via perturbation in dTDP-glucose synthesis in Escherichia coli. (2004)
  • Author(s): El-Kazzaz, W., Morita, T., Tagami, H., Inada, T., Aiba, H.
  • Journal Title: Mol.Microbiol. 51 Pages: 1117-1128
• [Journal Article] Mulyople domains of the mouse P19 (ARF)tumor suppressor are involved inp53-independent apoptosis. (2003)
  • Author(s): Matsuoka, M., Kurita, M., Sudo, H., Mizumoto. K. Nishimoto, I., Ogata, E.
  • Journal Title: Biochem. Biophys. Res. Commun. 301 Pages: 1000-1010
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] pl9ARF-induced p53-independent apoptosis largely occurs through BAX. (2003)
  • Author(s): Suzuki, H., Kurita, M., Mizumoto. K., Nishimoto, I., Ogata, E., Matsuoka, M.
  • Journal Title: Biochem. Biophys. Res. Commun. 312 Pages: 1273-1277
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Interaction of cellular tubulin with Sendai virus M protein regulatestranscription of viral genome. (2003)
  • Author(s): Ogino, T., Iwama, M., Ohsawa, Y., Mizumoto, K.
  • Journal Title: Biochem. Biophys. Res. Comm. n 311 Pages: 283-293
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Accumulation of glucose 6-phosphate or fructose 6-phosphate is responsible for destabilization of glucose transporter mRNA in Escherichia coli. (2003)
  • Author(s): Morita, T., El-Kazzaz, W., Tanaka. Y., Inada, T., Aiba, H.
  • Journal Title: J. Biol. Chem. 278 Pages: 15608-15614
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] SsrA-mediated trans-translation plays a role in mRNA quality control by facilitating degradation of truncated mRNAs. (2003)
  • Author(s): Yamamoto, Y., Sunohara. T., Jojima. K., Inada. T., Aiba. H.
  • Journal Title: RNA 9 Pages: 408-418
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Mulyople domains of the mouse PI 9 (ARF) tumor suppressor are involved in p53-independent apoptosis. (2003)
  • Author(s): Matsuoka, M., Kurita, M., Sudo, H., Mizumoto. K., Nishimoto. I., Ogata, E.
  • Journal Title: Biochem. Biophys. Res. Commun. 301 Pages: 1000-1010
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] pl9ARF-induced p53-independent apoptosis largely occurs through BAX. (2003)
  • Author(s): Suzuki, H., Kurita, M., Mizumoto. K.. Nishimoto. I., Ogata, E. & Matsuoka, M.
  • Journal Title: Biochem. Biophys. Res. Commun. 312 Pages: 1273-1277
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Interaction of cellular tubulin with Sendai virus M orotein regulates transcription of viral genome. (2003)
  • Author(s): Ogino, T., Iwama, M., Ohsawa, Y. & Mizumoto. K.
  • Journal Title: Biochem. Biophys. Res. Commn. 311 Pages: 283-293
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Accumulation of glucose 6-phosphate or fructose 6-phosphate is responsible for destabilization of glucose transporter mRNA in Escherichia coli (2003)
  • Author(s): Morita, T., El-Kazzaz, W., Tanaka, Y., Inada. T., Aiba, H.
  • Journal Title: J. Biol. Chem. 278 Pages: 15608-15614
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] SsrA-mediated transtranslation plays a role in mRNA quality control by facilitating degradation of truncated mRNAs. (2003)
  • Author(s): Yamamoto, Y., Sunohara, T., Jojima, K, Inada, T., Aiba, H.
  • Journal Title: RNA 9 Pages: 408-418
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] p53-independent apoptosis is induced by the pl9ARF tumor suppressor. (2002)
  • Author(s): Tsuji, K., Mizumoto. K., Sudo, H., Kouyama, K., Ogata, E., Matsuoka, M.
  • Journal Title: Biochem. Biophys. Res. Commun 295 Pages: 621-629
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Proteomic analysis of human brain identifies -enolase as a novel autoantigen in Hashimoto's encephalopathy. (2002)
  • Author(s): Ochi, H., Horiuchi, I., Araki, N., Toda, T., Araki, T., Sato, K., Murai, H., Osoegawa, M., Yamada, T., Okamura, K., Ogino, T., Mizumoto. K., Yamashita, H., Saya, H., J. Kira
  • Journal Title: FEBS Lett 528 Pages: 197-202
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Minimum molecular architectures for transcription and replication of the influenza virus. (2002)
  • Author(s): Honda, A., Mizumoto. K. &Ishihama, A.
  • Journal Title: Proc. Natl. Acad. Sci. USA 99 Pages: 13166-13171
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Interaction of S-adenosylhomocysteine hydrolase of Xenopus laevis withmRNA(guanine-7-)methyltransferase : Implication on its nuclear compartmentalisation and on cap methvlation of hnRNA. (2002)
  • Author(s): Radomski, N., Barreto, G., Kaufmann, C, Yokosuka, J., Mizumoto, K. & Dreyer, C.
  • Journal Title: Biochem. Biophys. Acta 1590 Pages: 93-102
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Differential effect of ik3-I/Cables on p53- and p73-induced cell death. (2002)
  • Author(s): Tsuji, K., Mizumoto. K, Yamochi, T., Nishimoto, I., Matsuoka, M.
  • Journal Title: J. Biol. Chem. 277 Pages: 2951-2957
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] The C-tertninal amino acid sequence of nascent peptide is a major determinant of SsrA tagging at all three stop codons. (2002)
  • Author(s): Sunohara. T., Abo, T., Inada. T. Aiba. H.
  • Journal Title: RNA 8 Pages: 1416-1427
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] One-Step affinity purification of the yeast ribosome and its associated proteins and mRNAs. (2002)
  • Author(s): Inada. T., Winstall, E., Tarun, S. Z., Jr., Yates, J. R., 3rd, Schieltz, D., Sachs, A. B.
  • Journal Title: RNA 8 Pages: 948-958
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] p53-independent apoptosis is induced by the pl9ARF tumor suppressor. (2002)
  • Author(s): Tsuji, K., Mizumoto. K., Sudo, H., Kouyama, K., Ogata, E. & Matsuoka, M.
  • Journal Title: Biochem. Biophys. Res. Commun. 295 Pages: 621-629
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Proteomic analysis of human brain identifies -enolase as a novel autoantigen in Hashimoto's encephalopathy. (2002)
  • Author(s): Ochi, H., Horiuchi, I., Araki, N., Toda, T., Araki, T., Sato, K., Murai, H., Osoegawa, M., Yamada, T., Okamura, K., Ogino, T., Mizumoto. K., Yamashita, H., Saya, H., J. Kira
  • Journal Title: FEBS Lett. 528 Pages: 197-202
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Minimum molecular architectures for transcription and replication of the influenza virus. (2002)
  • Author(s): Honda, A., Mizumoto. K. & Ishihama, A.
  • Journal Title: Proc. Nail. Acad. Sci. USA 99 Pages: 13166-13171
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Interaction of S-adenosylhomocysteine hydrolase of Xenopus laevis with mRNA(guanine-7-)methyltransferase: Implication on its nuclear compartmentalisation and on cap methylation of hnRNA. (2002)
  • Author(s): Radomski, N., Barreto, G., Kaufmann, C, Yokosuka, J., Mizumoto. K. & Dreyer, C.
  • Journal Title: Biochem. Biophys. Acta 1590 Pages: 93-102
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Differential effect of ik3-l/Cables on p53- and p73-induced cell death. (2002)
  • Author(s): Tsuji, K., Mizumoto. K., Yamochi, T., Nishimoto, I., Matsuoka, M.
  • Journal Title: J. Biol. Chem. 277 Pages: 2951-2957
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The C-terminal amino acid sequence of nascent peptide is a major determinant of SsrA tagging at all three stop codons. (2002)
  • Author(s): Sunohara, T., Abo, T., Inada. T., Aiba, H.
  • Journal Title: RNA 8 Pages: 1416-1427
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] One-step affinity purification of the yeast ribosome and its associated proteins and mRNAs. (2002)
  • Author(s): Inada. T., Winstall, E., Tarun, S. Z., Jr., Yates, J. R., 3rd, Schieltz, D., Sachs, A. B.
  • Journal Title: RNA 8 Pages: 948-958
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Analysis of capped RNA by boronate affinity PAGE (2006)
  • Author(s): Yoshio Shibagaki, Kiyohisa Mizumoto
  • Organizer: 20th IUBMB International Congress of Biochemistry and Morecular Biology and nth FAOBMB Congress
  • Place of Presentation: Kyoto, Japan
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] The mechanism of capping of RNA polymerase II transcripts - The timing of cap formation (2006)
  • Author(s): Tetsuro Komatsu, Yoshio Shibagaki, Kiyohisa Mizumoto
  • Organizer: 20th IUBMB International Congress of Biochemistry andMorecular Biology and nth FAOBMB Congress
  • Place of Presentation: Kyoto, Japan
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Characterizaation of cap-dependent endonuclease activity associated with influenzavirus RNA polymerase - the comparison of type A and type B viruses (2006)
  • Author(s): Chitose Wakai, Takatoshi Saito, MinakoIwama, Yujiro Suzuki, Kiyohisa Mizumoto
  • Organizer: 20th IUBMB International Congress of Biochemistry and Morecular Biology and l 1th FAOBMB Congress
  • Place of Presentation: Kyoto, Japan
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Identification of ribosome complex that stalls at the 3'end of nonstop mRNA and represses further rounds of translation (2006)
  • Author(s): Toshifumi Inada
  • Organizer: Translational Control Meeting (Cold Spring Harbor Laboratory)
  • Place of Presentation: Cold Spring Harbor Laboratory, NY, USA
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Analysis of capped RNA by boronate affinity PAGE (2006)
  • Author(s): Yoshio Shibagaki, Kiyohisa Mizumoto
  • Organizer: 20th IUBMB International Congress of Biochemistry and Morecular Biology and 11th FAOBMB Congress
  • Place of Presentation: Kyoto
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] The mechanism of capping of RNA polymerase II transcripts - The timing of cap formation (2006)
  • Author(s): Tetsuro Komatsu, Yoshio Shibagaki, Kiyohisa Mizumoto
  • Organizer: 20th IUBMB International Congress of Biochemistry and Morecular Biology and 11th FAOBMB Congress
  • Place of Presentation: Kyoto
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Characterizaation of cap-dependent endonuclease activily associated with influenza vims RNA polymerase.- the comparison of type A and type B viruses (2006)
  • Author(s): Chitose Wakai, Takatoshi Saito, Minako Iwama, Yujiro Suzuki, Kiyohisa Mizumoto
  • Organizer: 20th IUBMB International Congress of Biochemistry and Morecular Biology and 11th FAOBMB Congress
  • Place of Presentation: Kyoto
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Identification of ribosome complex that stalls at the 3'end of nonstop mRNA and represses further rounds of translation (2006)
  • Author(s): Toshifumi Inada.
  • Organizer: Translational Control Meeting
  • Place of Presentation: NY
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Catalytic Properties of Capped RNA Endonuclease Associated with InfluenzaVirus RNA Polym erase - The Comparison of Type A and Type B Viruses (2005)
  • Author(s): Wakai C, Saito T, Iwama M, Suzuki Y, Mizumoto K
  • Organizer: XIIIth International Congress of Virology
  • Place of Presentation: San Francisco, USA
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Catalytic Properties of Capped RNA Endonuclease Associated with Influenza Virus RNA Polymerase - The Comparison of Type A and Type B Viruses (2005)
  • Author(s): Wakai C, Saito T, Iwama M, Suzuki Y, Mizumoto K
  • Organizer: XIIIth International Congress of Virology
  • Place of Presentation: San Francisco
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Functional analysis of helicase and kinase activities of TFIIH (2005)
  • Author(s): Hisatake K, Fukuda A, Shibagaki Y, Nakafuo T, Shimada M, Mizumoto K, Nogi Y
  • Organizer: The 26th Annual Meeting The Molecular Biology Society of Japan, Symposium
  • Place of Presentation: Kobe
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] The fate of stalled ribosome at 3' end of truncated mRNA : Surveillance systems for nonstop mRNA in eukaryotes and prokaryotes. (2004)
  • Author(s): Toshifumi Inada
  • Organizer: Translational Control (Cold Spring Harbor Meeting)
  • Place of Presentation: Cold Spring Harbor Laboratory, NY, USA
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Analysis of protein factors required for in vitro transcription of Sendai virus genome. (2004)
  • Author(s): Mizumoto K
  • Organizer: The 25th Annual Meeting The molecular Biology Society of Japan, Workshop
  • Place of Presentation: Yokohama
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] The fate of stalled ribosome at 3' end of truncated mRNA : Surveillance systems for nonstop mRNA in eukaryotes and prokaryotes. (2004)
  • Author(s): Toshifumi Inada.
  • Organizer: Translational Control Meeting
  • Place of Presentation: NY
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] インフルエンザウイルスRNAポリメラーゼの活性と特異性の制御 (2003)
  • Author(s): 本田文江, 岡本拓人, 水本清久, 清水一史, 宮尾憲孝, 石浜明
  • Organizer: 日本ウイルス学会第51回学術集会・総会ワークシヨップ
  • Place of Presentation: 京都、日本
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] センダイウイルスRNA依存RNAポリメラーゼ(Lタンパク質)はウイルス特異的のmRNAのキャップをメチル化する (2003)
  • Author(s): 荻野朝朗, 小林正樹, 岩間美奈子, 水本清久
  • Organizer: 国際シンポジウム「RNA研究のフロンティア」
  • Place of Presentation: Kyoto, Japan
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 転写初期反応におけるRNA polymerase II によるmRNAキャップ化酵素の役割 (2003)
  • Author(s): 柴垣芳夫, 野島孝之, 松井博紀, 久武幸司, 福田綾, 水本清久
  • Organizer: 国際シンポジウム「RNA研究のフロンティア」
  • Place of Presentation: Kyoto, Japan
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] THIIHのヘリガーゼとキナーゼ活性の機能解析 (2003)
  • Author(s): 久武幸司, 福田綾, 柴垣芳夫, 中太智義, 嶋田美穂, 水本清久, 禾康壽
  • Organizer: 第26回日本分子生物学会年会シンポジウム
  • Place of Presentation: 神戸、日本
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Translation of nonstop mRNA in S. cerevisiae (2003)
  • Author(s): Toshifumi Inada
  • Organizer: RNA2003 kyoto
  • Place of Presentation: Kyoto, Japan
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Analysis of ribosome association and protein products of nonstop mRNA in S. cerevisiae (2003)
  • Author(s): Toshifumi Inada
  • Organizer: RNAmeeting 2003
  • Place of Presentation: Vienna, Austria
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Involvement of mRNA capping enzyme in transcription initiation by RNA polymerase II (2003)
  • Author(s): Shibagaki Y, Nojima T, Matsui H, Hisatake K, Fukuda A, Mizumoto K.
  • Organizer: RNA2003 Kyoto
  • Place of Presentation: Kyoto
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Sendai virus RNA-dependent RNA polymerase (L protein) catalyzes cap methylation of virus-specific mRNA (2003)
  • Author(s): Ogino T, Kobayashi M, Iwama M, Mizumoto K.
  • Organizer: RNA2003 Kyoto
  • Place of Presentation: Kyoto
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Translation of nonstop mRNA in S. cerevisiae. (2003)
  • Author(s): Toshifumi Inada.
  • Organizer: RNA2003 Kyoto
  • Place of Presentation: Kyoto
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Analysis of ribosome association and protein products, of nonstop mRNA in S.cerevisiae (2003)
  • Author(s): Toshifumi Inada.
  • Organizer: RNA meeting 2003.
  • Place of Presentation: Vienna
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] センダイウイルスゲノムの転写に関与する宿主因子群の解析 (2002)
  • Author(s): 水本清久
  • Organizer: 第25回日本分子生物学会年会フーグショップ
  • Place of Presentation: 横浜、日本
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Regulation of activities and specificity of influenza vims RNA polymerase (2002)
  • Author(s): Honda F, Okamoto T, Mizumoto K, Shimizu K, Miyao K, Ishihama A.
  • Organizer: The 51st Annual Meeting of The Japanese Society for Virology
  • Place of Presentation: Kyoto
  • Description: 「研究成果報告書概要(欧文)」より
• [Book] 「正常と異常を識別する品質管理機構」蛋白質核酸酵素『遺伝暗号解読40周年』 (2006)
  • Author(s): 稲田利文
  • Publisher: 共立出版
  • Description: 「研究成果報告書概要(和文)」より
• [Book] 「細胞の危機管理システムー細胞を脅かす不良RNAへの対応」 科学 (2006)
  • Author(s): 稲田利文
  • Publisher: 岩波書店
  • Description: 「研究成果報告書概要(和文)」より
• [Book] 「mRNA品質管理機構におけるpoly(A)鎖の新たな役割」、蛋白質核酸酵素増刊『躍進するRNA研究』 (2006)
  • Author(s): 稲田利文
  • Publisher: 共立出版
  • Description: 「研究成果報告書概要(和文)」より
• [Book] 「mRNAの品質管理機構」 化学と生物 (2006)
  • Author(s): 稲田利文
  • Publisher: 日本農芸化学会学会誌
  • Description: 「研究成果報告書概要(和文)」より
• [Book] 「最も早いタンパク質品質管理機構」実験医学増刊『細胞内タンパク質の社会学』 (2005)
  • Author(s): 稲田利文,饗場弘二
  • Publisher: 羊土社
  • Description: 「研究成果報告書概要(和文)」より
• [Book] 応用微生物学IV、微生物の遺伝・育種、4、「翻訳とその制御」 (2005)
  • Author(s): 稲田利文
  • Publisher: 文永堂出版
  • Description: 「研究成果報告書概要(和文)」より
• [Book] 「RNAとは」『RNA工学の最前線』 (2005)
  • Author(s): 稲田利文
  • Publisher: シーエムシー出版
  • Description: 「研究成果報告書概要(和文)」より
• [Book] 「ゲノムからみた翻訳とRNAの品質管理」ゲノム医学特別企画 (2004)
  • Author(s): 松藤千弥,稲田利文
  • Publisher: メディカルレビュー社
  • Description: 「研究成果報告書概要(和文)」より
• [Book] 「mRNA品質管理機構における翻訳の新たな役割」実験医学増刊、『RNA研究の新展開』 (2004)
  • Author(s): 稲田利文
  • Publisher: 羊土社
  • Description: 「研究成果報告書概要(和文)」より
• [Book] 「RNAと品質管理」わかる実験医学シリーズ「RNAがわかる」トピックス遍第2章 (2003)
  • Author(s): 稲田利文
  • Publisher: 羊土社
  • Description: 「研究成果報告書概要(和文)」より
• [Book] 新しいRNA機能による翻訳調節ネットワーク」実験医学 (2002)
  • Author(s): 稲田利文
  • Publisher: 羊土社
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Ogino, T., Iwama, M., Ohsawa, Y., Mizumoto, K.: "Interaction of cellular tubulin with Sendai virus M protein regulates transcription of viral genome."Biochem.Biophys.Res.Commun.. 311. 283-293 (2003)
• [Publications] Suzuki, H., Kurita, M., Mizumoto, K., Nishimoto, I., Ogata, E., Matsuoka, M.: "p19ARF-induced p53-independent apoptosis largely occurs through BAX."Biochem.Biophys.Res.Commun.. 312. 1273-1277 (2003)
• [Publications] Yamamoto, T., Sunohara, T., Jojima, K., Inada, T., Aiba, H.: "SsrA-mediated trans-translation plays a role in mRNA quality control by facilitating degradation of truncated mRNAs."RNA. 9. 408-418 (2003)
• [Publications] Baron-Benhamou, J., Fortes, P., Inada, T., Preiss, T., Hentze, MW.: "The interaction of the cap-binding complex(CBC) with elF4G is dispensable for translation in yeast."RNA. 9. 654-662 (2003)
• [Publications] Morita, T., El-Kazzaz, W., Tanaka, Y., Inada, T., Aiba, H.: "Accumulation of glucose 6-phosphate or fructose 6-phosphate is responsible for destabilization of glucose transporter mRNA in Escherichia coli."J.Biol.Chem.. 278. 15608-15614 (2003)
• [Publications] Sunohara, T., jojima, K., Yamamoto, Y., Inanda, T., Aiba, H.: "Nascent peptide-mediated ribosome stalling at stop codons induces mRNA cleavages resulting in nonstop mRNAs that are recognized by tmRNA."RNA. 10. 378-386 (2004)
• [Publications] Sunohara, T., Jojima, K., Tagami, H., Inada, T., Aiba, H.: "Ribosome stalling during translation elongation induces cleavage of mRNA being translated in Escherichia Coli."J.Biol.Chem.. (in press). (2004)
• [Publications] Tadauchi, T., Inada, T., Matsumoto, K., Irie, K.: "Post-transcriptional regulation of HO expression by the Mktl-Pbp1 complex."Mol.Cell Biol.. (in press). (2004)
• [Publications] Tsuji, K., Mizumoto, K., Yamochi, T., Nishimoto, I., Matsuoka M.: "Differential effect of ik3-1/Cables on p53-and p73-induced cell death"J.Biol.Chem.. 277. 2951-2957 (2002)
• [Publications] Radomski, N., Barreto, G., Kaufmann, C., Yokosuka, J., Mizumoto, K., Dreyer.C.: "Interaction of S-adenosylhomocysteine hydrolase of Xenopus laevis with mRNA(guanine-7-)methyltransferase : Implication on its nuclear compartmentalisation and on cap methylation of hnRNA"Biochem.Biophys.Acta. 1590. 93-102 (2002)
• [Publications] Honda, A., Mizumoto, K., Ishihama, A.: "Minimum molecular architectures for transcription and replication of the influenza virus"Proc.Natl.Acad.Sci.USA. 99. 13166-13171 (2002)
• [Publications] Ochi, H., Horiuchi, I., Araki, N., Toda, T., Araki, T., Sato, K., Murai, H., Osoegawa, M., Yamada, T., Okamura, K., Ogino, T., Mizumoto, K., Yamashita, H., Saya, H., Kira, J.: "Proteomic analysis of human brain identifies α-enolase as a novel autoantigen in Hashimoto's encephalopathy"FEBS Letters. 528. 197-202 (2002)
• [Publications] Inada, T., Winstall, E., Tarun, S.Z., Jr., Yates, J.R., 3rd, Schieltz, D., Sachs, A.B.: "One-step affinity purification of the yeast ribosome and its associated proteins and mRNAs"RNA. 8. 948-958 (2002)
• [Publications] Sunohara, T., Abo, T., Inada, T., Aiba, H.: "SsrA tagging at stop codons occurs depending on amino acid sequence of C-terminal end of the nascent peptide"RNA. 8. 1416-1427 (2002)