| Project/Area Number |
14035237
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | Kobe University (2003-2006)
Nara Institute of Science and Technology (2002) |
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Principal Investigator |
INOUE Kunio Kobe University, Faculty of Science, Associate Prof. (40252415)
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| Co-Investigator(Kenkyū-buntansha) |
HIRAOKA Yasushi Kobe Advanced ICT Research Center, 関西先端研究センター, Projent leader (10359078)
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| Project Period (FY) |
2002 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥67,500,000 (Direct Cost: ¥67,500,000)
Fiscal Year 2006: ¥12,400,000 (Direct Cost: ¥12,400,000)
Fiscal Year 2005: ¥12,400,000 (Direct Cost: ¥12,400,000)
Fiscal Year 2004: ¥14,200,000 (Direct Cost: ¥14,200,000)
Fiscal Year 2003: ¥14,200,000 (Direct Cost: ¥14,200,000)
Fiscal Year 2002: ¥14,300,000 (Direct Cost: ¥14,300,000)
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| Keywords | zabrafish / germ plasm / mRNA localization / germ cell / micro RNA / translational regulation / microRNA(miRNA) / ミトコンドリア凝集体 / 母性mRNA / ポリA鎖 / RNA情報発現 / 発生分化 / RNA結合蛋白質 / mRNP |
|---|
| Research Abstract |
mRNA localization plays important roles in spatio-temporal control of eukaryotic gene expression. Using zebrafish as a model organism, we succeed to show the following points: (i) the maternally supplied cytoplasm containing localized mRNAs, such as vasa, nanosl and dazl, functions as the germ cell determinant in zebrafish, (ii) the zebrafish "germ plasm" mRNAs are transported to the vegetal pole via mitochondrial clouds during early oogenesis, (iii) unlocalized nanosl and tdrd7 mRNAs are subjected to enhanced destabilization and translation repression via deadenylation or poly(A) tail shortening by one of the abundantly expressed microRNA, miR-430, in somatic cells, and also (iv) miR-430 plays a key role in maternal-to-zygotic transition, repressing maternally supplied mRNAs in zebrafish embryos.
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