側生器官の軸に依存した器官発生機構の解析

• Project/Area Number: 14036220
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Kyoto University
• Principal Investigator: 岡田 清孝 京都大学, 大学院理学研究科, 教授 (50101093)
• Co-Investigator(Kenkyū-buntansha): 槻木 竜二 京都大学, 大学院理学研究科, 助手 (50303805) ; 松本 任孝 京都大学, 大学院・理学研究科, 助手 ; 石黒 澄衞 京都大学, 大学院・理学研究科, 助手 (50260039)
• Project Period (FY): 2002 – 2006
• Project Status: Completed (Fiscal Year 2006)
• Budget Amount: ¥130,200,000 (Direct Cost: ¥130,200,000); Fiscal Year 2006: ¥25,000,000 (Direct Cost: ¥25,000,000); Fiscal Year 2005: ¥25,000,000 (Direct Cost: ¥25,000,000); Fiscal Year 2004: ¥26,600,000 (Direct Cost: ¥26,600,000); Fiscal Year 2003: ¥26,600,000 (Direct Cost: ¥26,600,000); Fiscal Year 2002: ¥27,000,000 (Direct Cost: ¥27,000,000)
• Keywords: 器官形成 / 軸構造 / シロイヌナズナ / 遺伝子発現 / 分裂組織 / 細胞分裂 / プロモーター解析 / 花形成 / 細胞分化

Research Abstract

葉や花弁などの側生器官の背腹を決定する場合には分裂組織と器官原基との相対位置が重要な要因となる。本研究では、シロイヌナズナの突然変異体を用いて、器官の基本構造決定の遣伝的な制御機構を解析した。
(1)側生器官の背側マーカー遺伝子PHBの発現領域を解析した。PHBの発現領城は、PHBpromoter::YFPの発現と、GFP遺伝子内部にmicroRNA165/166による認識・切断配列を挿入して35Sプロモーターにつないだ場合の発現を比較した。PHBp::YFPは茎頂分裂組織のほぼ全域で発現しているが、35S::GFP-miRsiteの発現は原基が盛り上がる前の葉原基の予定領域では低下しており、その場所で腹側マーカー遺伝子FILが発現していた。この結果は、葉原基形成の極初期に表側と裏側領域が区別されて特異的遺伝子の発現領域の境界が決定されており、microRNAが活性を持っていることを示している。また、後期球状胚においてPHBp::YFPが胚の上部ほぼ全域で発現するのに対し、35S::GFP-miRsiteはV型の発現パターンを示したことから、胚発生の初期においてもmicroRNAが機能発現すると考えられる。
(2)FIL遺伝子の発現パターンが変化する突然変異体の解析を進めた。#2-07-4変異株ではFILの発現領域が広くなるが、維管束組織の位置はほぼ不変であった。この結果は、葉断面における維管束の位置はFIL-PHB発現領域の決定機構とは独立に決められることを示唆する。
(3)葉の周縁部の細胞列形成に必要でこの細胞列で特異的に発現するPRESSED FLOWER遺伝子および花弁原基でのみ特異的に発現領域するRABBIT EARS遺伝子の特異的発現機構を調べるために、プロモーター領域を細分して機能解析をおこない、特異的発現を促す領域を約200塩基対に縮めた。

Research Products

• [Journal Article] Histone Deacetylases and ASYMMETRIC LEAVES2 Are Involved in the Establishment of Polarity in Leaves of Arabidopsis. (2007)
  • Author(s): Ueno Y.
  • Journal Title: Plant Cell 19 Pages: 445-457
• [Journal Article] A traial of phenome analysis using 4000 Ds-insertional mutants in gene-coding regions of Arabidopsis. (2006)
  • Author(s): Takashi Kuromori
  • Journal Title: Plant J. 47 Pages: 640-651
• [Journal Article] Regulation of CAPRICE transcription by Myb proteins for epidermis differentiation in Arabidopsis. (2005)
  • Author(s): Yoshihiro Koshino-Kimura
  • Journal Title: Plant & Cell Physiology 46 Pages: 817-826
• [Journal Article] An Arabidopsis rhomboid homolog is an intramembrane protease in plants. (2005)
  • Author(s): Masahiro Kanaoka
  • Journal Title: FEBS Letters 579 Pages: 5723-5728
• [Journal Article] The Arabidopsis STV1 protein, responsible for translation reinitiation, is required for auxin-mediated organ patterning. (2005)
  • Author(s): Taisuke Nishimura
  • Journal Title: The Plant Cell 17 Pages: 2940-2953
• [Journal Article] Cell-to-cell movement of the CAPRICE protein in the Arabidopsis root epidermal cell differentiation. (2005)
  • Author(s): Tetsuya Kurata
  • Journal Title: Development 132 Pages: 5387-5398
• [Journal Article] Two callose synthases, GSL1 and GSL5, play an essential and redundant role in plant and pollen development and in fertility. (2005)
  • Author(s): Linda C.Enns
  • Journal Title: Plant Molec.Biol. 58 Pages: 333-349
• [Journal Article] Root Development 〜 Stepwise Understanding of Root Patterning 〜 Current Opinion in Plant Biology (2005)
  • Author(s): Minako Ueda
  • Journal Title: Current Opinion in Plant Biology 8 Pages: 71-76
• [Journal Article] The Arabidopsis WAV2 protein modulates root bending in response to environmental stimuli (2005)
  • Author(s): Susumu Moshizuki et al.
  • Journal Title: Plant Cell 17 Pages: 537-547
• [Journal Article] Root Development 〜 Stepwise Understanding of Root Patterning 〜 (2005)
  • Author(s): Minako Ueda, et al.
  • Journal Title: Current Opinion in Plant Biology 8 Pages: 71-76
• [Journal Article] The ARABIDOPSIS COW1 gene encodes a phosphatidyl-inositol transfer protein essential for root hair tip growth. (2004)
  • Author(s): Karen Bohme, et al.
  • Journal Title: Plant Journal 40 Pages: 686-698
• [Journal Article] RABBIT EARS encoding a SUPERMAN-like zinc finger protein regulates petal and ovule development in Arabidopsis thaliana (2004)
  • Author(s): Seiji Takeda, et al.
  • Journal Title: Development 131 Pages: 425-434
• [Journal Article] The HALTED ROOT (HLR) gene encoding the 26S proteasome subunit AtRPT2a is essential for the maintenance of Arabidopsis meristems (2004)
  • Author(s): Minako Ueda, et al.
  • Journal Title: Development 131 Pages: 2101-2111
• [Journal Article] A key factor of transcription reinitiation, ribosomal protein L24, is involved in gynoecium development in Arabidopsis. (2004)
  • Author(s): T.Nishimura, et al.
  • Journal Title: Biochemical Society Transactions 32 Pages: 611-613
• [Book] モデル植物の実験プロトコール -イネ・シロイヌナズナ・ミヤコグサ編- (2005)
  • Author(s): 島本 功
  • Total Pages: 324
  • Publisher: 秀潤社
• [Book] 植物の環境応答と形態形成のクロストーク(岡 穆宏, 岡田清孝, 篠崎一雄 編) (2004)
  • Author(s): 酒井達也, 岡田清孝, 高野誠
  • Total Pages: 230
  • Publisher: シュプリング・フェアラーク東京
• [Publications] Keiro Watanabe, Kiyotaka Okada: "Two discrete cis elements control the abaxial side-specific expression of the FILAMENTOUS FLOWER gene in Arabidopsis"Plant Cell. 15. 2592-2603 (2003)
• [Publications] Taisuke Nishimura, et al.: "An Arabidopsis ACT2 dominant-negative mutation, which disturbs F-actin polymerization, reveals its distinctive function in root development."Plant & Cell Physiology. 44. 1131-1140 (2003)
• [Publications] Tetsuya Kurata, et al.: "The YORE-YORE gene regulates multiple aspects of epidermal cell differentiation in Arabidopsis"Plant J.. 36. 55-66 (2003)
• [Publications] Seiji Takeda, et al.: "RABBIT EARS encoding a SUPERMAN-like zinc finger protein regulates petal and ovule development in Arabidopsis thaliana"Development. 131. 425-434 (2004)
• [Publications] Minako Ueda, et al.: "The HALTED ROOT (HLR) gene encoding the 26S proteasome subunit AtRPT2a is essential for the maintenance of Arabidopsis meristems"Development. 132(In press). (2004)
• [Publications] Maki Ogishi, et al.: "Gain-of-function analysis of blue light receptors using multiple mutants in Arabidopsis"Proc.Natl.Acad.Sci.USA. 101(In press). (2004)
• [Publications] Keiro Watanabe, et al.: "Axis-dependent regulation of lateral organ-development in plants."Morphogenesis and Pattern Formation in Biological Systems - Experiments and Models. 398 (2003)
• [Publications] Sumie Ishiguro et al.: "SHEPHERD is the Arabidopsis GRP94 responsible for the formation of functional CLAVATA proteins"EMBO Journal. 21. 898-908 (2002)
• [Publications] Eiko Kanaya et al.: "Non-sequence-specific DNA binding by the Filamentous flower protein from Arabidopsis thaliana is reduced by EDTA"J.Biol.Chem.. 277. 11957-11964 (2002)
• [Publications] Tokitaka Oyama et al.: "The IRE gene encodes a protein kinase homologue and modulates root hair growth in Arabidopsis"Plant J.. 30. 289-299 (2002)
• [Publications] Takeji Wada et al.: "Role of the positive regulator of root hair development, CAPRICE, in the epidermal cell differentiation of Arabidopsis root"Development. 129. 5409-5419 (2002)
• [Publications] S.Schellmann et al.: "TRIPTYCHON and CAPRICE mediate lateral inhibition during trichome and root patterning in Arabidopsis"EMBO J.. 21. 5036-5046 (2002)
• [Publications] Nobuyuki Takahashi et al.: "Hydrotropism in abscisic acid, wavy, and gravitropic mutants of Arabidopsis thaliana"Planta. 216. 203-211 (2002)
• [Publications] 岡田清孝 他4名編: "蛋白質核酸酵素増刊号「植物の形づくり」"共立出版. 326 (2002)