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Studies on the mechanism of ovulation in vertebrates by post-genomic approaches

Research Project

Project/Area Number 14204079
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field 生物形態・構造
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

TAKAHASHI Takayuki  Hokkaido Univ., Grad.School of Sci., Prof., 大学院・理学研究科, 教授 (80197152)

Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥45,760,000 (Direct Cost: ¥35,200,000、Indirect Cost: ¥10,560,000)
Fiscal Year 2004: ¥11,180,000 (Direct Cost: ¥8,600,000、Indirect Cost: ¥2,580,000)
Fiscal Year 2003: ¥15,210,000 (Direct Cost: ¥11,700,000、Indirect Cost: ¥3,510,000)
Fiscal Year 2002: ¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000)
Keywordsovulation / ovary / medaka / mechanism of ovulation / matrix metalloproteinases / 排卵酵素 / マトリクスメタロプロテアーゼ / MT2-MMP / マウス / 排卵酸素
Research Abstract

Ovulation, which is triggered by a preovulatory surge of luteinizing hormone (LH) released from the pituitary gland, is a dynamic process that results in the liberation of a mature fertilizable ovum from the ovarian follicle. This event, which is known as follicle rupture, has been the subject of intensive investigation over the past century. Previous studies, which have been carried out mainly in mammalian species, including humans, have established that follicle rupture is accomplished by dissolution of the granulosa cell basement membrane and fragmentation of the collagenous matrix at the apex of the follicular wall, thereby implicating proteolytic enzymes. Indeed, a variety of proteases have been proposed as candidates for rupturing the follicle. However, the proteases that are essential for follicle rupture in ovulation have not yet been identified, despite much effort. In this study, we investigated vertebrate ovulatory processes in the medaka, with the particular aim of identifying the enzymes responsible for the proteolytic degradation of the follicle walls. We found that follicle rupture in the medaka ovary involves the cooperation of at least three matrix metalloproteinases (MMPs), together with the tissue inhibitor of metalloproteinase-2b (TIMP-2b) protein. We determined the discrete roles of each of these proteins during follicle rupture. Our results indicate that gelatinase A induces the hydrolysis of type IV collagen constituting the basement membrane, membrane-type 2 (MT2) MMP degrades type I collagen present in the theca cell layer, and MT1-MMP and TIMP-2b are involved in the production and regulation of gelatinase A. The in vitro ovulation system made it possible to identify proteases that are critical for ovulation in the medaka, and the use of similar experimental systems should help to identify ovulatory enzymes in mammals.

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (23 results)

All 2005 2004 2003 2002 Other

All Journal Article (15 results) Patent(Industrial Property Rights) (1 results) Publications (7 results)

  • [Journal Article] Gelatinase A and membrane-type matrix metalloproteinases 1 and 2 are responsible for follicle rupture during ovulation in the medaka, Oryzias latipes.2005

    • Author(s)
      Ogiwara, K.
    • Journal Title

      Proceedings of the National Academy of Sciences (U.S.A.) (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Gelatinase A and membrane-type matrix metalloproteinases 1 and 2 are responsible for follicle rupture during ovulation in the medaka, Oryzias latipes.2005

    • Author(s)
      Ogiwara, K.et al.
    • Journal Title

      Proceedings of the National Academy of Sciences (USA) (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Expression of proprotein convertase 2 mRNA in the ovarian follicles of the medaka, Oryzias latipes.2004

    • Author(s)
      Ogiwara, K.
    • Journal Title

      Gene 337

      Pages: 79-89

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Structure and expression of furin mRNA in the ovary of medaka fish, Oryzias latipes.2004

    • Author(s)
      Ogiwara, K.
    • Journal Title

      Journal of Experimental Zoology 301A

      Pages: 449-459

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Expression of proprotein convertase 2 mRNA in the ovarian follicles of the medaka, Oryzias latipes.2004

    • Author(s)
      Ogiwara, K.et al.
    • Journal Title

      Gene 337

      Pages: 79-89

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Structure of and expression of furin mRNA in the ovary of medaka Fish, Oryzias latipes.2004

    • Author(s)
      Ogiwara, K.et al.
    • Journal Title

      Journal of Experimental Zoology 301A

      Pages: 449-459

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Structure and expression of furin mRNA in the ovary of medaka fish, Oryzias latipes2004

    • Author(s)
      Ogiwara, K.
    • Journal Title

      Journal of Experimental Zoology 301A

      Pages: 449-459

    • Related Report
      2004 Annual Research Report
  • [Journal Article] メダカのin vitro排卵系実験系を利用した排卵研究2003

    • Author(s)
      荻原克益
    • Journal Title

      日本比較内分泌学会ニュース 111

      Pages: 52-55

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Expression of bradykinin B2 receptor in the mouse ovary.2003

    • Author(s)
      Ohkura, R.
    • Journal Title

      Zoological Science 20

      Pages: 847-854

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Studies on the ovulatory mechanism in the medaka using an in vitro ovulation experimental system. (Japanese)2003

    • Author(s)
      Ogiwara, K.et al.
    • Journal Title

      Newsletter of Japan Society for Compararative Endocrinology 111

      Pages: 52-55

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Expression of bradykinin B2 receptor in the mouse ovary.2003

    • Author(s)
      Ogiwara, K.et al.]
    • Journal Title

      Zoologizal Science 20

      Pages: 847-854

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Molecular cloning and partial characterization of medaka fish stromelysin-3 and its restricted expression in the oocytes of small growing follicles of the ovary.2002

    • Author(s)
      Ogiwara, K.
    • Journal Title

      Molecular Reproduction and Development 61

      Pages: 21-31

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The presence of angiotensin-converting enzyme in follicular fluids of porcine ovaries and its possible involvement in the intrafollicular breakdown of bradykinin.2002

    • Author(s)
      Matsui, H.
    • Journal Title

      Molecular Reproduction and Development 62(1)

      Pages: 99-105

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Molecular cloning and partial characterization of medaka fish stromelysin-3 and its restricted expression in the oocytes of small growing follicles of the ovary.2002

    • Author(s)
      Ogiwara, K.et al.
    • Journal Title

      Molecular Reproduction and Development 61

      Pages: 21-31

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The presence of angiotensin-converting enzyme in follicular fluids of porcine ovaries and its possible involvement in the intrafollicular breakdown of bradykinin.2002

    • Author(s)
      Ogiwara, K.et al.
    • Journal Title

      Molecular Reproduction and Development 62

      Pages: 99-105

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Patent(Industrial Property Rights)] 魚類由来のエンテロペプチダーゼ2004

    • Inventor(s)
      高橋 孝行, 荻原 克益
    • Industrial Property Rights Holder
      北海道TLO
    • Industrial Property Number
      2004-066632
    • Filing Date
      2004
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Publications] Ohkura, R: "Expression of Bradykinin B2 Receptor in the Mouse Ovary"Zoological Science. 20. 847-854 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ogiwara, K: "Structure and Expression of Furin mRNA in the Ovary of Medaka Fish, Oryzias latipes"Journal of Experimental Zoology. (印刷中). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 荻原克益: "メダカのin vitro排卵系実験系を利用した排卵研究"日本比較内分泌学会ニュース. 111. 52-55 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ogiwara, K.: "Molecular Cloning and Partial Characterization of Medaka Fish Stromelysin-3 and Its Restricted Expression in the Oocytes of Small Growing Follicles of the Ovary"Mol. Reprod. Develop.. 61. 21-31 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kimura, A.: "Expression and Localization of Prolyl Oligopeptidase in Mouse Testis and Its Possible Involvement in Sperm Motility"Zool. Sci.. 19. 93-102 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Matsui, H.: "The Presence of Angiotensin-converting Enzyme in Follicular Fluids of Porcine Ovaries and Its Possible Involvement in Intrafollicular Breakdown of Bradykinin"Mol. Reprod. Develop.. 62. 99-105 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yokoi, T.: "Developmental Changes and Localization of Mouse Brain Serine Proteinase mRNA and Protein in Mouse Brain"Neurosci. Lett.. 323. 133-136 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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