Subsurface transport of microorganism and its application to bioremediation of groundwater contaminated by halogenated organic compound

• Project/Area Number: 14205067
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Geotechnical engineering
• Research Institution: Gifu University
• Principal Investigator: SATO Takeshi Gifu University, Faculty of Engineering, Professor, 工学部, 教授 (80135326)
• Co-Investigator(Kenkyū-buntansha): TAKAMIZAWA Kazuhiro Gifu University, Faculty of Applied Biological Science, Professor, 応用生物科学部, 教授 (00159005)
• Project Period (FY): 2002 – 2004
• Project Status: Completed (Fiscal Year 2004)
• Budget Amount: ¥35,750,000 (Direct Cost: ¥27,500,000、Indirect Cost: ¥8,250,000); Fiscal Year 2004: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2003: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000); Fiscal Year 2002: ¥23,530,000 (Direct Cost: ¥18,100,000、Indirect Cost: ¥5,430,000)
• Keywords: anaerobic microorganism / bio-augmentation / bioremediation / groundwater / advection-dispersion / Michaelis-Menten equation / biodegradation kinetics / numerical simulation / ミカエリスメンテン式 / 数値シミュレーション / Clostridium bifermentans DPH-1 / リアルタイム定量的PCR法 / DNA濃度 / 分散係数 / カラム実験 / 堆積速度 / 遅れ係数 / DNAマイクロアレイ / スタントン数 / ペクレ数 / Two Regionモデル / FISH / 定量的PCR

Research Abstract

Laboratory column tests confirmed that the transport characteristics of microorganism, Clostridium bifermentans DPH-1 activating tetrachloroethylene (PCE) dechlorination, was well described by a mobile-immobile two-region model (TRM), which has been used in physical transport model of aqueous pollutants through soils. Determined TRM parameters implied that bacterial injection into ground at large amount of concentration is difficult in a rehabilitation manner for widely dispersed contaminated groundwater. Calibrated parameters were carefully evaluated in conjunction with test conditions and features of the soils tested in column. Practical estimation were proposed in transport parameters to simulate bacteria transport and degradation of PCE in bio-augmentation. Biological reaction was identified as first-order kinetics from batch tests where the bacterium strain was mixed with halogenated aliphatic compounds of PCE or TCE (trichloroethylene) as initial pollutant. PCE dechlorination to cis-dichloroethylene (cDCE) via TCE was mathematically described by Monod type function based on Michaelis-Menten equation while the microbial growth rate was not well represented by Monod function. Thus, a relationship between the microbial growth rate and the concentration of PCE and TCE was newly proposed and its appropriateness was verified through a numerical simulation of PCE dechlorination in a fixed-bed reactor column.

Research Products

• [Journal Article] Transport of Clostridium bifermentans DPH-1 through the laboratory column can be explained by two-region model for bioremediation (2005)
  • Author(s): Humayra, A.S., Kimura, Y., Takamizawa, K., Sato, T.
  • Journal Title: Environment Technology (in press)
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Evaluation of different culture conditions of Clostridium bifermentan DPH-1 for cost effective PCE degradation (2005)
  • Author(s): Humaya, A.S., Hasegawa, Y., Nomura, I., Chang, Y.C., Sato, T., Takamizawa, K.
  • Journal Title: Biotechnology and Bioprocess Engineering 10 Pages: 40-46
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Transport of Clostridium bifermantans DPH-1 through the laboratory column can be explained by two-region model for bioremediation (2005)
  • Author(s): Humayra, A.S., Kimura, Y, Takamizawa, K., Sato, T.
  • Journal Title: Environment Technology (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Evaluation of different culture conditions of Clostridium bifermentans DPH-1 for cost effective PCE degradation (2005)
  • Author(s): Humayra, A.S., Hasegawa, Y., Nomura, I., Chang, Y.C., Sato, T., Takamizawa, K.
  • Journal Title: Biotechnology and Bioprocess Engineering 10 Pages: 40-46
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Evaluation of different culture conditions of Clostridium bifermentans DPH-1 for cost effetctive PCE deqradation (2005)
  • Author(s): Humayra, A.S., Hasegawa, Y, Nomura, I., Chang, Y.C., Sato, T., Takamizawa, K
  • Journal Title: Biotechnology and Bioprocess Engineering (in press)
• [Journal Article] 嫌気性微生物を用いたPCE汚染土壌・地下水の修復 (2004)
  • Author(s): 高見澤一裕, 佐藤健
  • Journal Title: 水 46-5・655 Pages: 26-34
  • NAID: 40006121155
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Anaerobic degradation of cis- 1,2,-dichloroethylene and vinyl chloride by Clostridium sp.Strain DC1 isolated from landfill leachate sediment. (2004)
  • Author(s): Hata, J., Miyata, N., Kim, E-S., Takamizawa, K., Iwahori, K.
  • Journal Title: Journal of Bioscience and Bioengineering 97 Pages: 196-201
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Quantification of Bacillus species in a wastewater treatment system by the molecular analyses (2004)
  • Author(s): Mori, K., Irie, R., Hirata, M., Takamizawa, K.
  • Journal Title: Biotechnology and Bioprocess Engineering 9 Pages: 482-489
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Ribotyping and a study of transmission of Staphylococcus aureus collected from food preparation facilities (2004)
  • Author(s): Kishimoto, M., Konuma, H., Takamizawa, K., Kashio, H., Kasuga, F et al.
  • Journal Title: Journal of Food Protection 67 Pages: 1116-1122
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Anaerobic degradation of cis-1,2,-dichloroethylene and vinyl chloride by Clostridium sp.Strain DC1 isolated from landfill leachate sediment (2004)
  • Author(s): Hata, J., Miyata, N., Kim, E-S., Takamizawa, K., Iwahori, K.
  • Journal Title: Journal of Bioscience and Bioengineering 97 Pages: 196-201
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Qualification of Bacillus species in a wastewater treatment system by the molecular analyses (2004)
  • Author(s): Mori, K., Irie, R., Hirata, M., Takamizawa, K.
  • Journal Title: Biotechnology and Bioprocess Engineering 9 Pages: 482-489
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Ribotyping and a study of transmission of Staphylococcus aureus collected from food preparation facilities (2004)
  • Author(s): Kishimoto, M., Konuma, H., Takamizawa, K., Kashio, H.Kasuga, F et al.
  • Journal Title: Journal of Food Protection 67 Pages: 1116-1122
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Anaerobic degradation of cis-1,2,-dichloroethylene and vinyl chloride by Clostridium sp. Strain DC1 isolated from landfill leachate sediment. (2004)
  • Author(s): Hata, J., Miyata, N., Kim, E-S., Takamizawa, K., Iwahori, K.
  • Journal Title: Journal of Bioscience and Bioengineering 97 Pages: 196-201
• [Journal Article] 調理施設から採取された黄色ブドウ球菌のRAPD-PCR、BSFGEおよびPFGEによる遺伝子多型解 (2004)
  • Author(s): 岸本満, 高見澤一裕, 春日文子ら
  • Journal Title: 日本食品微生物学会誌 21 Pages: 193-200
• [Journal Article] Ribotyping and a study of transmission of Staphylococcus auneus collected from food preparation facilities. (2004)
  • Author(s): Kishimoto, M., Konuma, H., Takamizawa, K., Kashio, H.Kasuga, F et al.
  • Journal Title: Journal of Food Protection 67 Pages: 1116-1122
• [Journal Article] Solute dispersion in a variably saturated sand (2003)
  • Author(s): Sato, T., Tanahashi, H., Loaiciga, H.A
  • Journal Title: Water Resources Research 39-6
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Transport and characteristics of biodegradation Clostridium bifermentans DPH-1 (2003)
  • Author(s): Sato, T., Kimura, Y., Takamizawa, K
  • Journal Title: Proc.8th International FZK/TNO Conference on Contaminated Soil (CD-ROM)
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Solute dispersion in a variably saturated sand (2003)
  • Author(s): Sato, T.Tanahashi, H., Loaiciga, H.A.
  • Journal Title: Water Resources Research 39-6
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Transport and characteristics of biodegradation Clostridium bifermentans DPH-1 (2003)
  • Author(s): Sato, T., Kimura, Y., Takamizawa, K.
  • Journal Title: Proc.8th International FZK/TNO Conference on Contaminated Soil (CD-ROM)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Modeling transport and bio-degradation of PCE in sandy soil (2002)
  • Author(s): Sato, T., Kimura, Y., Takamizawa, K
  • Journal Title: Proc.of the first International Conference on Brownfield Sites Pages: 515-524
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Modeling transport and bio-degradation of PCE in sandy soil (2002)
  • Author(s): Sato, T., Kimura, Y., Takamizawa, K.
  • Journal Title: Proc.of the first International Conference on Brownfield Sites Pages: 515-524
  • Description: 「研究成果報告書概要(欧文)」より
• [Book] 微生物工学 (2004)
  • Author(s): 菊池慎太郎, 高見澤一裕, 浦野直人, 海藤晃弘, 藤井克彦
  • Total Pages: 169
  • Publisher: 三共出版
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Hata, J., Takamizawa, K., Miyata, N., Iwahori, K.: "Biodegradation of cis-1,2-dichloroethylene and vinyl chloride in anaerobic cultures enriches from landfill leachate sediment under Fe(III)-reducing conditions"Biodegradation. 14. 275-283 (2003)
• [Publications] Hata, J., Miyata, N., Kim, E.S., Takamizawa, K., Iwahori, K.: "Anaerobic degradation of cis-1,2-dichloroethylene and vinyl chloride by Clostridium sp.strain DC1 isolated from landfill leachate sediment"J.Biosci.Bioeng.. 97(印刷中). (2004)
• [Publications] Sato, T., Tanahashi, H., Loaiciga, H.A.: "Solute dispersion in a variably saturated sand"Water Resources Research. 39,6. 7-1-7-7 (2003)
• [Publications] 鈴木孝男, 佐藤健: "遺伝的アルゴリズムに基づく排水工法の最適井戸配置"地下水技術. 44・6. 32-39 (2002)
• [Publications] T.Sato, Y.Kimura, K.Takamizawa: "Modeling transport and biodegradation of PCE in sandy soil"Proc. Of the first Int. Conf. On Brownfield Sites. 515-524 (2002)
• [Publications] 佐藤健, 木村由郎, 高見澤一裕: "PCE分解微生物の土中移動特性とバイオレメディエーションへの応用"土と基礎(地盤工学会誌). 50・10. 19-21 (2002)
• [Publications] 佐藤健, 高見澤一裕: "Clostridium bifermentans DPH-1 株の土中移動特性と有機塩素化合物分解特性に基づくバイオメレディエーションの設計"Proc. Of the 5^<th> Osaka-Seoul Symp. On Environmental Issues. 31-36 (2002)