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Diagnosis, epidemiology and prevention of flavivirus infection including West Nile fever.

Research Project

Project/Area Number 14206036
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied veterinary science
Research InstitutionHokkaido University

Principal Investigator

TAKASHIMA Ikuo  Hokkaido University, Graduate School of Veterinary Medicine, Professor, 大学院・獣医学研究科, 教授 (30002083)

Co-Investigator(Kenkyū-buntansha) KARIWA Hiroaki  Hokkaido University, Graduate School of Veterinary Medicine, Associate Professor, 大学院・獣医学研究科, 助教授 (70224714)
MORITA Koichi  Institute for Tropical Medicine, Nagasaki University, Professor, 熱帯医学研究所, 教授 (40182240)
TADANO Masayuki  Medical School, Ryuku University, Associate Professor, 医学部, 助教授 (80179712)
TAKEGAMI Tsutomu  Kanazawa Medical Schools, Institute for Medical Sciences, Professor, 総合医学研究所, 教授 (10113490)
ESHITA Yuki  Ohita Medical University, Faculty of Medicine, Associate Professor, 医学部, 助教授 (10082223)
水谷 哲也  国立感染症研究所, 主任研究員 (70281681)
Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥43,550,000 (Direct Cost: ¥33,500,000、Indirect Cost: ¥10,050,000)
Fiscal Year 2004: ¥11,830,000 (Direct Cost: ¥9,100,000、Indirect Cost: ¥2,730,000)
Fiscal Year 2003: ¥13,130,000 (Direct Cost: ¥10,100,000、Indirect Cost: ¥3,030,000)
Fiscal Year 2002: ¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
KeywordsWest Nile fever / Flavivirus infection / diagnosis / epidemiology
Research Abstract

In order to prevent the outbreaks of flavivirus infections, we developed the diagnostic tests, examined pathogenecity of the viruses and examined vector susceptibility of the Japanese mosquitoes. The results are summarized as follows.
1.Development of diagnostic tests.
1)As the genetic diagnostic tests of West Nile virus, RT-PCR RFLP method, a real time PCR method and RT-LAMP method were developed.
2)For tick-borne encephalitis, IgG-and IgM-ELISA using subviral particles were developed as serological diagnostic tests for human.
2.Studies on pathogenecity of flavivirus
1)Neuroinvasive virulence of West Nile virus New York strain was found to be associated with glysosylation of envelope protein.
2)Neuroinvasive virulence of tick-borne encephalitis virus was reduced due to one amino acid change of envelope protein which led to the reduced vircemia.
3.Japanese encephalitis
1)Japanese encephalitis virus from South-East Asian countries often invaded into Japan.
2)RNAi was found to be effective as antiviral agent to Japanese encephalitis viris.
4.Mosquito studies
1)Japanese indigenous mosquitoes of 4 species were found to be susceptible to West Nile virus.

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (36 results)

All 2004 2003 2002 2000 Other

All Journal Article (28 results) Publications (8 results)

  • [Journal Article] Viral envelop protein glycosylation is a molecular determinant of the neuroinvasiveness of the New York strain of West Nile virus.2004

    • Author(s)
      Shirato, K., et al.
    • Journal Title

      J.Gen.Virol. 85(12)

      Pages: 3637-3645

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Single point mutation in tick-borne encephalitis virus prM protein induces a reduction of virus particle secretion.2004

    • Author(s)
      Yoshii, K., et al.
    • Journal Title

      J.Gen.Virol. 85(10)

      Pages: 3049-3058

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Amino acid changes responsible for attenuation of virus neurovirulence in an infectious cDNA clone of the Oshima strain of Tick borne encephalitis virus.2004

    • Author(s)
      Hayasaka, D., et al.
    • Journal Title

      J.Gen.Virol. 85(4)

      Pages: 1007-1018

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Sub-genomic-replicons of Tick-borne encephalitis virus.2004

    • Author(s)
      Hayasaka, D., Yoshii, K., Ueki, T., Iwasaki, T., Takashima, I.
    • Journal Title

      Arch Virol. 149

      Pages: 1245-1256

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Amino acid chages responsible for attenuation of virus neurovirulence in an infectious cDNA clone of the Oshima strain of Tick-borne encephalitis virus.2004

    • Author(s)
      Hayasaka, D., Gritsun, T.S, Yoshii, K., Ueki, T., Goto, A., Mizutani, T., Kariwa, H., Iwasaki, T., Gould, E.A., Takashima, I.
    • Journal Title

      J Gen Virol. 85

      Pages: 1007-1018

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Single point mutation in tick-borne encephalitis virus prM protein induces a reduction of virus particle secretion.2004

    • Author(s)
      Yoshii, K., Konno, A., Goto, A., Nio, J., Obara, M., Ueki, T., Hayasaka, D., Mizutani, T., Kariwa, H., Takashima I
    • Journal Title

      J Gen Virol. 85

      Pages: 3049-3058

    • NAID

      120000958376

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Different chemokine expression in lethal and non-lethal murine West Nile virus infection.2004

    • Author(s)
      Shirato, K., Kimura, T., Mizutani, T., Kariwa, H., Takashima, I.
    • Journal Title

      J Med Virol. 74

      Pages: 507-513

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Real-time reverse transcription Loop-Mediated isothermal amplification for rapid detection of West Nile virus.2004

    • Author(s)
      Parida, M., Posadas, G., Inoue, S., Hasebe, F., Morita, K.
    • Journal Title

      J Cli Micro 42

      Pages: 257-263

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Shift in Japanese encephalitis virus (JEV) genotype circulating in northrn Vietnam : implications for frequent introductions of JEV from Southeast Asia to East Asia.2004

    • Author(s)
      Nga, P.T., Parquet, M.C., Cuong, V.D., Ma, S-P., Hasebe, F., Inoue, S., Makino, Y., Takagi, M., Nam, V.S., Morita, K.
    • Journal Title

      J Gen Virol. 85

      Pages: 1625-1631

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Fusion PCR generated Japanese encephalitis virus/dengue 4 virus chimera exhibits lack of neuroinvasiveness, attenuated neurovirulence, a dual-flavi immune response in mice.2004

    • Author(s)
      Mathenge, E.G.M., Parquet, M.C., Funakoshi, Y., Houhara, S., Wong P.F., Ichinose, A., Hasebe, F., Inoue, S., Morita, K.
    • Journal Title

      J Gen Virol. 85

      Pages: 2503-2513

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Nucleotide sequence and phylogenetic analyzes of dengue type 2 virus isolated in the Dominican Republic.2004

    • Author(s)
      Anzai, S., Fukuda, M., Otuka, Y., Eshita, Y.
    • Journal Title

      Virus Genes. 29

      Pages: 219-227

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Seroepidemiology of dengue and assessment of public awareness in the Dcominican Republic.2004

    • Author(s)
      Makino, Y., Shichijo, A., Bello, C., Eshita, Y., Disla, M., Cesin, A.J., Galcla, B., Lora, M., Valdez, S., Aquino, J.D., Aono, H., Ma, S-P., Takeshita, M.
    • Journal Title

      Tropical Med and Health. 32

      Pages: 305-309

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Viral envelope protein glycosylation is a molecular determinant of the neuroinvasiveness of the New York strain of West Nile virus.2004

    • Author(s)
      Shirato, K., Miyoshi, H, Goto, A., Ako, Y., Ueki, T., Kariwa,.H, Takashima, I.
    • Journal Title

      J Gen Virol 85

      Pages: 3637-3645

    • NAID

      120000953678

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Amino acid changes responsible for attenuation of virus neurovirulence in an infectious cDNA clone of the Oshima strain of Tick-borne encephalitis virus.2004

    • Author(s)
      Hayasaka, D., et al.
    • Journal Title

      J.Gen.Virol. 85(4)

      Pages: 1007-1018

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Involvement of the JNK-like protein of the Aedes albopictus mosquito cell line, C6/36, in phagocytosis, endocytosis and infection of West Nile virus.2003

    • Author(s)
      Mizutani, T., et al.
    • Journal Title

      Insect Molecular Biology 12(5)

      Pages: 491-499

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Discrimination of West Nile virus and Japanese encephalitis virus atrains using RT-PCR RFLP analysis.2003

    • Author(s)
      Shirato, K., et al.
    • Journal Title

      Microbiol.Immunol. 47(6)

      Pages: 439-445

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Enzyme-linked immunosorbent assay using reconbinant antigens exeressed in mammalisn cells for serodiagnosis of tick-borne encephalitis.2003

    • Author(s)
      Yoshii, K., et al.
    • Journal Title

      J.Virol.Methods. 108(2)

      Pages: 171-179

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Enzyme-linked immunosorbent assay using recombinant antigens expressed in mammalian cells for serodiagnosis of tick-borne encephalitis.2003

    • Author(s)
      Yoshii, K., Hayasaka, D., Goto, A., Obara, M., Araki, K., Yoshimatsu, K., Arikawa, J., Ivanov, L.I., Mizutani, T., Kariwa, H., Takashima, I.
    • Journal Title

      J.virol.Methods 108(2)

      Pages: 171-179

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Discrimination of West Nile Virus and Japanese encephalitis virus strains using RT-PCR RFLP analysis.2003

    • Author(s)
      Shirato, K., Mizutani, T., Kariwa, H., Takashima, I.
    • Journal Title

      Microbiol Immunol. 47

      Pages: 439-445

    • NAID

      80015950178

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Characterization of JNK-like protein derived from a mosquito cell line, C6/36.2003

    • Author(s)
      Mizutani, T., Kobayashi, M., Eshita, Y., Inanami, O., Yamamori, T., Goto, A., Miyoshi, H., Miyamoto, H., Kariwa, H., Kuwabara, M, Takashima, I.
    • Journal Title

      Ins Mol Biol. 12

      Pages: 61-66

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Distribution of three arbovirus antibodies among monkeys (Macaca fascicularis)in the Philippines.2003

    • Author(s)
      Inoue, S., Morita, K., Matias, R.R., Tuplano, J.V., Resuello, R.R.G., Gandelario, J.R., Cruz, D.J.M., Mapua, C.A., Hasebe, F., Igarashi, A., Natividad F.F.
    • Journal Title

      J.Med.Primatology. 32

      Pages: 89-94

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Serodiagnosis of Japanese encephalitis among Nepalese patients by the particle agglutination assay.2003

    • Author(s)
      Pandey, B., Yamamoto, A., Marita, K., Kurosawa, Y., Rat, S., Adhikari, S., Kandel, P., Kurane, I.
    • Journal Title

      Epidemiol.Infect. 131

      Pages: 881-885

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Involvement of the JNK-like protein of the Aedes albopictus mosquito cell line, C6/36, in phagocytosis, endocytosis and infection of West Nile virus.2003

    • Author(s)
      Mizutani, T., et al.
    • Journal Title

      Insect Molecular Biology. 12(5)

      Pages: 491-499

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Enzyme-linked immunosorbent assay using neconbinant antigens exeressed in mammalisn cells for serodiagnosis of tick-borne encephalitis.2003

    • Author(s)
      Yoshii, K., et al.
    • Journal Title

      J.Virol.Methods. 108(2)

      Pages: 171-179

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Genetic and biological comparison of tick-borne encephalitis viruses from Hokkaido and Far-Eastern Russia. I.2002

    • Author(s)
      Goto, A., Hayasaka, D., Yoshii, K., Mizutani, T., Kariwa, H, Takashima, I.
    • Journal Title

      Jpn.J.Vet.Res. 49

      Pages: 297-307

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] St. Louis encephalitis virus induced pathology in cultured cells.2002

    • Author(s)
      M.C.Parquet, Kumatori, A., Hasebe, F., E.G.M.Mathenge., Morita, K.
    • Journal Title

      Arch of virol. 147

      Pages: 1105-1119

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Infection and dissemination of two dengue type-2 viruses isolated from patients exhibiting different disease severity in orally infected Aedes aegypti from different geographic origin.2002

    • Author(s)
      R.E.Morales, Marita, K., Eshita, Y., Tsuda, Y., Fukuma, T., M, Takagi.
    • Journal Title

      Med.Entomol.Zool. 53

      Pages: 21-27

    • NAID

      110003819160

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Involvement of the JNK-like protein of the Aedes albopictus mosquito cell line, C6/36, in phagocytosis, endocytosis and infection of West Nile virus.2000

    • Author(s)
      Mizutani, T., Kobayashi, M., Eshita, Y., Shirato, K., Kimura, T., Ako, Y., Miyoshi, H., Takasaki, T., Kurane, I., Kariwa, H, Umemura, T., Takashima, I.
    • Journal Title

      Ins Mol Biol. 12

      Pages: 491-499

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Mizutani, T., et al.: "Involvement of the JNK-like protein of the Aedes albopictus mosquito cell line, C6/36, in phagocytosis, encdocytosis and infection of West Nile virus."Insect Molecular Biology. 12(5). 491-499 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yoshii, K., et al.: "Enzyme-linked immunosorbent assay using recombinantantigens expressed in mammalian cells for serodiagnosis of tick-borne encephalitis."J.Virol.Method. 108(2). 171-179 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Mizutani, T., et al.: "Characterization of JNK-like protein derived from mosquito cell line, C6/36."Insect Mol.Biol. 12(1). 61-66 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Shirato, K., et al.: "Discrimination of West Nile Virus and Japanese Encephalitis Virus Strains Usibg RT-PCR RFLP Analysis."Microbiol Immunol. 47(6). 439-445 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Goto, A., et al.: "A BHK-21 cell culture-adapted tick-borne encephalitis virus mutant is attenuated for neuroinvasiveness."Vaccine. 21. 4043-4051 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Goto, A., et al.: "Genetic and biological comparison of tick-borne encephalitis viruses from Hokkaido and Far-Eastern Russia"Jpn.J.Vet.Res. 49. 297-307 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yoshii, K., et al.: "Development of enzyme-linked immunosorbent assay using recombinent antigens expressed in mamalian cells for serodiagnosis of tick-borne encephalitis"J.Virol.Method. 107(in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Mizutani, T., et al.: "Characterization of JNK-like protein derived from mosquito cell line, C6/36"Insect Mol.Biol. 12(1). 61-66 (2003)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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