| Budget Amount *help |
¥53,040,000 (Direct Cost: ¥40,800,000、Indirect Cost: ¥12,240,000)
Fiscal Year 2005: ¥13,000,000 (Direct Cost: ¥10,000,000、Indirect Cost: ¥3,000,000)
Fiscal Year 2004: ¥13,000,000 (Direct Cost: ¥10,000,000、Indirect Cost: ¥3,000,000)
Fiscal Year 2003: ¥13,000,000 (Direct Cost: ¥10,000,000、Indirect Cost: ¥3,000,000)
Fiscal Year 2002: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
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| Research Abstract |
1) We elucidated the mechanism underlying the site-specific chromatin remodeling around the GAGA factor-binding site. First, GAGA factor recruits FACT to its binding site. Then, FACT induces a conformational change in the neighboring nucleosomes, which in turn facilitates chromatin remodeling.
2) We identified subunits of the Brahma chromatin remodeling compex as the GAGA factor-associated proteins besides FACT, suggesting that Brahma complex is involved in the GAGA factor-FACT complex induced chromatin remodeling.
3) Genetic analysis revealed that the GAGA factor-FACT complex contributes to epigenetic maintenance of Hox gene expression.
4) Genetic and biochemical analyses demonstrated that the GAGA factor-FACT complex is involved in position effect variegation.
5) We found a crucial role of Drosophila supercoiling factor in the dosage compensation of X chromosome using RNAi in vivo.
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