| Project/Area Number |
14208107
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY |
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Principal Investigator |
OHGUSHI Hajime NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY (AIST), TISSUE ENGINEERING RESEARCH GROUP RESEARCH INSTITUTE FOR CELL ENGINEERING, GROUP LEADER, セルエンジニアリング研究部門, 研究グループ長 (80213669)
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| Co-Investigator(Kenkyū-buntansha) |
ITO Atsuo NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY (AIST), 主任研究員 (30356480)
KODAMA Makoto KYUSYU INSTITUTE OF TECHNOLOGY, GRADUATE SCHOOL OF LIFE SCIENCE AND SYSTEMS ENGINEERING, 大学院・生命体工学研究科, 助教授 (70344447)
KANEMURA Yonehiro NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY (AIST), 研究員 (80344175)
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| Project Period (FY) |
2002 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥54,210,000 (Direct Cost: ¥41,700,000、Indirect Cost: ¥12,510,000)
Fiscal Year 2004: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2003: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2002: ¥45,890,000 (Direct Cost: ¥35,300,000、Indirect Cost: ¥10,590,000)
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| Keywords | Mesenchymal stem cells / Bone marrow / Bone regeneration / Tissue engineering / Culture / Hepatocyte / Neuron |
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| Research Abstract |
Stem cells have a remarkable potential for developing into many different cell types in the body. When a stem cell divides, each new cell has the ability to either remain a stem cell (self-renewal) or become another type of cell with a more specialized function (specific cell lineage commitment). Stem cells derived from postnatal animals (especially humans) are called adult stem cells and are generally thought to be multipotent stem cells.
Among adult stem cells, mesenchymal stem cells (MSCs) are also multipotent cells that can be isolated from adult bone marrow and can be induced in vitro and in vivo to differentiate into a variety of mesenchymal tissues, including bone and cartilage. Here, we report that we have succeeded to proliferate the number of MSCs from adult human as well as rat and also induced theses MSCs into osteo/chondrogenic lineage. Importantly, the osteogenic cells in the pore regions of various porous ceramics can be used to repair osseous defects and we have used the cells for the clinical applications.
Interestingly, the MSCs can also differentiate into hepatocyte and neuron. When MSCs were cultured in the presence of hepatocyte growth factor, the cells showed albumin and c-Kit expression. When the MSCs were cultured in the presence of mercapto-ethanol, the cells' morphorogy resembled neuron and showed Nestin expression. Therefore, MSCs derived from bone marrow can be used to regenerate hepatic and neural function. These results indicate the usefulness of MSCs for tissue engineering and, thus, the cells hold promise for utilization in regenerative medicine.
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