| Project/Area Number |
14340121
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
物理学一般
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| Research Institution | Nagaoka University of Technology |
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Principal Investigator |
MATSUNO Koichiro Nagaoka University of Technology, Dept.of Engineering, Professor, 工学部, 教授 (10120346)
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| Co-Investigator(Kenkyū-buntansha) |
IMAI Eiichi Nagaoka University of Technology, Dept.of Engineering, Research & Technical Associate, 工学部, 教務職員 (30134977)
HATORI Kuniyuki Nagaoka University of Technology, Dept.of Engineering, Assistant Professor, 工学部, 助手 (00283036)
HONDA Hajime Nagaoka University of Technology, Dept.of Engineering, Associate Professor, 工学部, 助教授 (20192742)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥14,100,000 (Direct Cost: ¥14,100,000)
Fiscal Year 2003: ¥5,700,000 (Direct Cost: ¥5,700,000)
Fiscal Year 2002: ¥8,400,000 (Direct Cost: ¥8,400,000)
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| Keywords | oligonucleotide / oligopeptide / trimetaphosphate / Phosphorylation / flow reactor / Hvdrothermal Vent / chemical evolution / catalyst / 相互触媒 |
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| Research Abstract |
We used a flow reactor that was constructed for simulating the pressure and temperature conditions of the hydrothermal vents. We observed chemical evolution in an mixture of the four different kinds of amino acid including glycine, L-alanine, L-valine, L-aspatic acid circulated through the flow reactor. The HPLC profile of synthesized oligomers varied with each particular run, but the products were found to separate into distinct clusters. We found aspartic acid and its complexes with other amino acids have capabilities for making various products in subsequent reactions.
In hydrothermal environments allowing fluid circulation between hot and cold regions repeatedly, L-and D-alanine molecules were racemized differently depending upon the kinds and quantities of metallic ions available there. Hydrothermal environments in the primitive ocean could have maintained the capacity of selectively retaining enantiomeric excess in favor of L-amino acids.
Phosphorylation of AMP into ADP and ATP, that can outrun their hydrolysis, was made possible in a simulated hydrothermal environment when trimetaphosphate was used as the phosphate source. The AMP monomer could be oligomeraized to tri-or higher oligomers containing 3'-phosphodiester linkage as the operation time of the reactor increases.
These observations suggest that oligomeraization of amino acid and nucleotide molecules in hydrothermal environments could have produced those molecules mutually catalytic with each other.
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