| Project/Area Number |
14340256
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物生理
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| Research Institution | Okazaki National Research Institutes |
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Principal Investigator |
HAYASHI Makoto Okazaki National Research Institutes, National institute for Basic Biology, Department of Cell Biology, Associate professor, 基礎生物学研究所, 助教授 (50212155)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥15,000,000 (Direct Cost: ¥15,000,000)
Fiscal Year 2003: ¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2002: ¥11,300,000 (Direct Cost: ¥11,300,000)
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| Keywords | peroxisome / protein transport / lipid degradation / photorespiration / β-oxidation / glyoxysome / peroxin |
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| Research Abstract |
Peroxisomal proteins are transported from the cytosol into peroxisomes by a recognition of one of the two protein targeting signals called PTS1 and PTS2. Last year, we identified two receptors (Pex5p and Pex7p) that can capture PTS 1 and PTS2, and showed protein-protein interaction between Pex5p and Pex7p, as well as Pex5p and a peroxisomal membrane protein, Pex14p. In this fiscal year, we produced transgenic plants that suppressed PEX5-and PEX7-gene expression by RNA interference technology. Analyses of these transgenic plants showed following 2 points. (1) Loss of Pex5p inhibits the import of both PTS1-and PTS2-containing proteins into peroxisomes. The transgenic plants without Pex5p showed defects in functions of both glyoxysomes and leaf peroxisomes. (2) Loss of Pex7p inhibits the import of only PTS2-containing proteins. The transgenic plants without Pex7p showed defect only in glyoxysomal function. From these observation, we concluded that Pex5p and Pex7p form a receptor-cargo complex in the cytosol, and the receptor-cargo complex is captured by the protein-protein interaction between Pex5p and the peroxisomal membrane protein, Pex14p.
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