Microbial community analysis of enhanced biological phosphorus removal process and identification of polyphosphate accumulating organisms by molecular approach
| Project/Area Number |
14350283
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Civil and environmental engineering
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| Research Institution | The University of Tokyo |
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Principal Investigator |
MINO Takashi The University of Tokyo, Graduate school of Frontier Sciences, Professor, 大学院・新領域創成科学研究科, 教授 (60166098)
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| Co-Investigator(Kenkyū-buntansha) |
KURISU Futoshi The University of Tokyo, Graduate school of Engineering, Lecture, 大学院・工学系研究科, 講師 (30312979)
FURUMAI Hiroaki The University of Tokyo, Graduate school of Engineering, Professor, 大学院・工学系研究科, 教授 (40173546)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥15,000,000 (Direct Cost: ¥15,000,000)
Fiscal Year 2003: ¥6,600,000 (Direct Cost: ¥6,600,000)
Fiscal Year 2002: ¥8,400,000 (Direct Cost: ¥8,400,000)
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| Keywords | Activated sludge / anaerobic-aerobic / enhanced biologicall phosphorus removal / 16S-rDNA / DGGE / FISH / polyphosphate accumulating organisms / Rhodocyclus related PAOs |
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| Research Abstract |
Fundamental research about enhanced biological phosphorus removal (EBPR) process is now making great progress because of the new microbial community analysis methods such as molecular techniques. This research was carried to reveal the relationship between microbial community and function of EBPR sludge using 16S-rDNA approach.
In this research, at first, laboratory scale reactors were operated using acetate, glutamate, propionate, glucose or peptone as a main carbon source respectively. The expression of phosphorus removal activity was monitored by batch experiments and the microbial community change was monitored by Denaturing gradient gel electrophoresis (DGGE) using 16S-rDNA fragments. By comparing phosphorus removal activity and microbial community change, polyphosphate accumulating organisms were screened. In addition, by sequencing the whole length of 16S-rDNA of target bacteria by using cloning method and developing gene probes which can specifically detect the target bacteria and combining with the polyphosphate staining method and fluorescent in situ hybridization, capability of accumulating polyphosphate by screened bacteria was investigated.
As a result, it was demonstratedd that gram-positive bacteria with high GC content accumulated polyphosphate in the reactor fed with mainly peptone and this gram positive bacteria was considered as the second putative polyphosphate accumulating organisms (PAOs) after Rhodocyclus related bacteria, which has been already considered as PAOs candidates. In addition, as another bacteria which accumulated polyphosphate but was not Rhodocyclus related bacteria nor gram positive bacteria was detected, it was clearly shown that still unknown PAOs should exist. In this way, it was shown that the EBPR is achieved by the combination of several PAOs
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Report
(3 results)
Research Products
(4 results)
