Study of Chitin and Chitosan for Bio-medical Materials Application
| Project/Area Number |
14350504
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
高分子構造・物性(含繊維)
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| Research Institution | Kansai University |
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Principal Investigator |
TOKURA Seiichi Kansai University, Faculty of Engineering, Professor, 工学部, 教授 (40000806)
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| Co-Investigator(Kenkyū-buntansha) |
TAMURA Hiroshi Kansai University, Faculty of Engineering, Associate Professor, 工学部, 助教授 (20163694)
URAGAMI Tadashi Kansai University, Faculty of Engineering, Professor, 工学部, 教授 (80067701)
MIYATA Takashi Kansai University, Faculty of Engineering, Associate Professor, 工学部, 助教授 (50239414)
KIFUNE Kouji Koyo Chemical Co.Ltd, head of a department, 製造部長(研究職)
前田 睦浩 順天堂大学, 医学部, 講師 (10201766)
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| Project Period (FY) |
2002 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥15,100,000 (Direct Cost: ¥15,100,000)
Fiscal Year 2004: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2003: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 2002: ¥11,400,000 (Direct Cost: ¥11,400,000)
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| Keywords | chitin / calcium chloride dihydrate / solubility / wovens / fiber / drug carrier / degree of deacetylation / chitosan |
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| Research Abstract |
We have found that chitin dissolves in calcium chloride dihydrate saturated methanol to form a clear solution. In the briefly study, solubility behavior of chitin depend on the calcium, methanol and water content of the solution. Since the solubility of chitin decreased drastically in the medium of high water content, the chitin hydro gel appeared by the addition of water in the chitin solution. Obtained material was a highly swelled hydro gel containing more than 95 % of water. Dialysis was necessary to remove excess calcium from the hydro gel. In the same manner, addition of methanol in the chitin solution also gave gel like material. However, two fold amount of methanol was required to precipitate out from the chitin solution. In addition, long term dialysis was necessary to remove calcium from the gel material. These results suggest that the solubility condition is different from each other.
Properties of the chitin hydro gel were examined by several manners. Enzyme digestion rate of chitin hydro gel was compared with that of powder. Chitin hydro gel digested about ten times faster than powder using chitinase and lysozime. The results strongly suggests that chitin hydro gel has excellent biocompatible and biodigestive feature and is appropriate for bio medical usage. Removal of water from the chitin hydro gel regenerated chitin again. Based on the result, the removal of water by the use of conventional filtration apparatus gave chitin sheet. Once the water was removed from the chitin hydro gel, addition of water did not gave hydro gel again. The phenomenon may be carried out by the regeneration of hydrogen bond network between chitin molecules. The sheet is very interesting because this is a sheet made up of 100 % of chitin without of any glue. Dye adsorption examination afforded high preference for anionic dye, which suggest the possibility for the carrier of drug delivery system.
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Report
(4 results)
Research Products
(29 results)
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[Journal Article] Feasibility of Polysaccharide Hybrid Materials for Scaffolds in Cartilage Tissue Engineering : Evaluation of Chondrocyte Adhesion to Polyion Complex Fibers Prepared from Alginate and Chitosan2004
Author(s)
N.Iwasaki, S.Yamane, T.Majima, A.Minami, K.Harada, S.Nonaka, N.Maekawa, H.Tamura, S.Tokura, K.Monde, S.Nishimura
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Journal Title
Biomacromolecules Vol.5, No.3
Pages: 828-833
Description
「研究成果報告書概要(和文)」より
Related Report
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[Journal Article] Feasibility of Polysaccharide Hybrid Materials for Scaffolds in Cartilage Tissue Engineering : Evaluation of Chondrocyte Adhesion to Polyion Complex Fibers Prepared from Alginate and Chitosan2004
Author(s)
N.Iwasaki, S.Yamane, T.Majima, A.Minami, K.Harada, S.Nonaka, N.Maekawa, H.Tamura, S.Tokura, K.Monde, S.Nishimura
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Journal Title
Biomacromolecules 5(No.3)
Pages: 828-833
Description
「研究成果報告書概要(欧文)」より
Related Report
-
-
[Journal Article] Feasibility of Polysaccharide Hybrid Materials for Scaffolds in Cartilage Tissue Engineering : Evaluation of Chondrocyte Adhesion to Polyion Complex Fibers Prepared from Alginate and Chitosan2004
Author(s)
N.Iwasaki, S.Yamane, T.Majima, A.Minami, K.Harada, S.Nonaka, N.Maekawa, H.Tamura, S.Tokura, K.Monde, S.Nishimura
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Journal Title
Biomacromolecules Vol.5,No.3
Pages: 828-833
Related Report
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