| Project/Area Number |
14360013
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
園芸・造園学
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| Research Institution | Tohoku University |
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Principal Investigator |
SATOH Shigeru Tohoku University, Graduate School of Agricultural Sciences, Associate Professor, 大学院・農学研究科, 助教授 (40108428)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIOKA Toshihito Tohoku University, Graduate School of Agricultural Sciences, Assistant Professor, 大学院・農学研究科, 助手 (10240243)
KOSUGI Yosuke Kagawa University, Faculty of Agriculture, Assistant Professor, 農学部, 助手 (80325323)
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| Project Period (FY) |
2002 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥16,700,000 (Direct Cost: ¥16,700,000)
Fiscal Year 2004: ¥4,400,000 (Direct Cost: ¥4,400,000)
Fiscal Year 2003: ¥5,400,000 (Direct Cost: ¥5,400,000)
Fiscal Year 2002: ¥6,900,000 (Direct Cost: ¥6,900,000)
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| Keywords | long-lasting carnation / fower senescence / ehylene production / petal / wilting / fading / cysteine proteinase / promoter / 雌ずい / 遺伝子発現 / ACC合成酵素 / ACC酸化酵素 |
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| Research Abstract |
1.Analysis of ethylene production in the long-lasting carnation 'White Candle'
In senescing carnation (Dianthus caryophyllus L.) flowers, ethylene is produced from the gynoecium, and acts as a diffusible signal received by petals to induce autocatalytic ethylene production in the petals. We discovered cv. ‘White Candle (WC)' as a long-lasting cultivar, of which cut flowers produce ethylene only in trace amounts and have a long vase life. The low ethylene production in these flowers was due to low ethylene production in the gynoecium, accompanied by low accumulation of ACC synthase (DC-ACS1) transcripts. These findings further support the importance of ethylene production from the gynoecium in the senescence of carnation flowers.
2.Cloning and characterization of a cDNA for a novel carnation cysteine proteinase
The wilting of carnation petals is caused by degradation of cell components, which leads to cell death during senescence of petals, mediated by some hydrolytic enzymes such as cysteine proteinase (CPase, DC-CP1), lipase and so on. We isolated a cDNA for a novel cysteine proteinase (DC-CP2) from carnation and compared its expression to that of DC-CP1 in senescing carnation petals. Furthermore, we isolated and characterized the nucleotide sequences of promoter region of both DC-CP1 and DC-CP2 genes.
3.Analysis of wilting and fading in senescing carnation petals
We categorized senescence of carnation flowers was into two types ; the first is 'wilting' which is induced by the action of ethylene and the second is 'fading' which occurs independent on ethylene. We revealed that a cysteine proteinase (DC-CP1) and its inhibitor (DC-CPIn) were up-regulated in carnation petals undergoing wilting as well as fading.
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