Research of Bioavailability of Dietary Polyphenols in the Cells
| Project/Area Number |
14360076
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
食品科学・栄養科学
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| Research Institution | Kobe University |
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Principal Investigator |
KANAZAWA Kazuki Kobe University, Faculty of Agriculture, Professor, 農学部, 教授 (90031228)
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| Project Period (FY) |
2002 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥15,000,000 (Direct Cost: ¥15,000,000)
Fiscal Year 2004: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2003: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 2002: ¥11,400,000 (Direct Cost: ¥11,400,000)
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| Keywords | Polyphenols / Bioavailability / Flavonol / Caco-2 / Antioxidative potency / 8-OHdG / Artepillin C / Conjugation / 大腸がん / Caco-2細胞 / 抗酸化効果 / p21タンパク質 / CYP1A1 / アリール炭化水素受容体 / グルクロニド抱合 / ケンフェロール / ケルセチン / フラボノイド / HepG2 / ルテオリン |
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| Research Abstract |
Bioavailability of dietary polyphenols was investigated using intestinal Caco-2, hepatic HepG2 and colonic WiDr cells.
1.Apigenin-O-glucoside was incorporated into Caco-2 cells after hydrolyzed to aglycone, but its C-glucoside was not. The aglycone was released to the basolateral phase by 0.07% of the closed and its conjugated forms was by 1.1%.
2.Various flavonoids were added to HepG2 cells and determined in the incorporated amounts into nuclei. They gave the maximum incorporation at 30 and 60 min after incubation. When the nuclei were exposed to active oxygens, flavonoids that possessed catechol structure in B ring were available to suppress formation of a DNA-oxidized products 8-OHdG.
3.Flavonol type of flavonoids was specifically transformed to a novel metabolite, 3-amino forms, at the absorption step. The amino forms maintained the potencies for antioxidative activity and for protein fluction-modulating and additionally were refractory to be conjugated. Amino forms were considered to be an active form of flavonoids in the body.
4.When a mixture of various polyphenols was added to Caco-2 cells, a prenyl phenol artepillin C was easily incorporated and released to the basolateral phase without receiving any conjugations.
5.The artepillin C was also incorporated into HepG2 cells unchanged in form and suppressed the oxidation of intracellular DNA in a dose-dependent manner. Oral dose of artepillin C significantly prevent a formation of aberrant crypt foci in mouse colon. The dose of artepillin C to WiDr induced the cell arrest at G_0/G_1 phase through stimulating expression of p21.
Thus, flavonols and prenyl phenol were found to be bioavailable since the former was metabolized to more active forms and the latter was refractory to be conjugated in the absorption prpcess.
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Report
(4 results)
Research Products
(64 results)
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[Journal Article] Preventive effects of food components on caspase-8-mediated apoptosis induced by dietary carcinogen, Trp-P-1, in rat mononuclear cells.2003
Author(s)
Takashi Hashimoto, Wakana Ito, Takashi Furuyashiki, Takashi Sano, Ken-ichiro Minato, Masashi Mizuno, Kazuki Kanazawa, Gen-ichi Danno, Hitoshi Ashida
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Journal Title
American Chemical Society ACS Symposium Series 851
Pages: 128-140
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Suppression of cytochrome P4501 a subfamily in mouse liver by oral intake of polysaccharaides from mushroom, Lentinus edodes and Agaricus blazei.2003
Author(s)
Hitoshi Ashida, Takashi Hashimoto, Yuji Nonaka, Itsuko Fukuda, Kazuki Kanazawa, Gen-ichi Danno, Ken-ichiro Minato, Sachiko Kawakami, Masashi Mizuno
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Journal Title
American Chemical Society, ACS Symposium Series 851
Pages: 235-248
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Book] 栄養機能化学2005
Author(s)
金沢和樹
Total Pages
198
Publisher
朝倉書店
Description
「研究成果報告書概要(和文)」より
Related Report
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[Book] 色から見た食品のサイエンス2004
Author(s)
金沢和樹
Total Pages
342
Publisher
(株)サイエンスフォーラム
Description
「研究成果報告書概要(和文)」より
Related Report
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