| Project/Area Number |
14360097
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
林産学
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| Research Institution | Nagoya University |
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Principal Investigator |
FUKUSHIMA Kazuhiko Nagoya University, Graduate school of bioagricultural sciences, Professor, 大学院・生命農学研究科, 教授 (80222256)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUSHITA Yasuyuki Nagoya University, Graduate school of bioagricultural sciences, Assistant, 大学院・生命農学研究科, 助手 (60335015)
YASUDA Seiichi Nagoya University, Graduate school of bioagricultural sciences, Professor, 大学院・生命農学研究科, 名誉教授 (80002070)
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| Project Period (FY) |
2002 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥14,700,000 (Direct Cost: ¥14,700,000)
Fiscal Year 2004: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2003: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 2002: ¥10,900,000 (Direct Cost: ¥10,900,000)
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| Keywords | coniferin / coniferyl alcohol / coniferaldehyde / lignin / Ginkgo / biosynthesis / sinapic acid / hardwood / コニフェリアルアルコール |
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| Research Abstract |
Although recent molecular biological studies have led to the general idea of a "metabolic grid" for lignin biosynthesis, leading to novel findings in the study of lignin, many problems remain. One of these problems is the monolignol supply mechanism. To examine the storage and supply of monolignol in lignifying xylem tissue, we focused on coniferin (coniferyl alcohol glucoside) and coniferaldehyde glucoside. Tracer experiments using labeled glucosides showed that both glucosides were efficiently incorporated into lignin, and that a portion of the coniferin administered passed through an aldehyde form before polymerization into lignin. This suggests that there is another pathway by which coniferin joins the monolignol biosynthesis pathway, in addition to the pathway consisting of de-glucosylation catalyzed by β-glucosidase. Interestingly, syringyl units were rarely synthesized from coniferin via the cinnamyl alcohol stage, whereas numerous syringyl units were synthesized from coniferaldehyde glucoside. Based on these results, we propose a possible pathway in which some of the stored coniferin is oxidized to coniferaldehyde glucoside and de-glucosylation generates coniferaldehyde, which flows into the monolignol pathway.
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