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Development of the new vaccine and a delivery vector by the recombination Morbilli virus.

Research Project

Project/Area Number 14360186
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied veterinary science
Research InstitutionThe University of Tokyo

Principal Investigator

KOHARA Kyoko  The University of Tokyo, Institute of Medical Science, Lecturer, 医科学研究所, 講師 (20225478)

Co-Investigator(Kenkyū-buntansha) KAI Chieko  The University of Tokyo, Institute of Medical Science, Professor, 医科学研究所, 教授 (10167330)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥16,900,000 (Direct Cost: ¥16,900,000)
Fiscal Year 2003: ¥8,200,000 (Direct Cost: ¥8,200,000)
Fiscal Year 2002: ¥8,700,000 (Direct Cost: ¥8,700,000)
KeywordsNew Reverse Genetics method / Morbilli virus / vaccine / Deliver vector
Research Abstract

It aims at development of the safe recombination oral vaccine, half-oral vaccine and a new delivery vector by the new reverse genetics method which we established and which rearranges and makes development of a recombinant Morbilli virus possible in this research. This fiscal year mainly performed fundamental analysis of a virus vector using the rinderpest virus (RPV). First, it suggested that P protein was greatly concerned with the pathogenical change. when exchange the host using the RBOK and L strain of RPV. Moreover, to virus protein which governs pathogenicity was solved. Virus cloning established the highly pathogenic RPV-Lv strain and the weak pathogenic RPV-La strain out of the RPV-L strain. Consequently, among both, the amino acid substitution per site was found out in N, P, C, and L protein. When analyzed using seven sorts of viruses which rearranged the variation of N, P, and L genes of La strain with L strain, independently or simultaneous using the reverse genetics system … More , clinical condition was mitigated only for what changed N gene to La strain type. It became clear that N protein is important for pathogenesis of L strain. Moreover, in the B95a cell, although the vector which makes conditional expression system of M gene using a Cre/loxP system was produced for half-oral vaccine production, since the cell strain could nott be established. H gene mutated measles (MV) vector was tried to established, but a rescue was not succeeded, therefore, the new receptor binding unit which. is containing the penetration domain of the antibody recognition part gene (Fv) was induced in MV vector. They were obtained and analyzing whether an infection capacity has been changed. Furthermore, in order to analyze easily the delivery vector, the virus incorporating GFP and the luciferase gene was produced using the canine distemper virus (CDV) and MV vector. Among these, it succeeded in a MV-GFP vector visualizing the nerve cells which used the in vivo imaging equipment after inoculation in the mouse brain. Less

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (45 results)

All Other

All Publications (45 results)

  • [Publications] Kashiwagi et al.: "Kinetic analysis of C-terminally truncated RNA-dependent RNA polymerase of Hepatitis C virus."B.B.R.C.. 290. 1188-1194 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hirama, K. et al.: "Lymphocyte Activity Specific for Hemagglutinin (H) Protein of Canine Distemper Virus in Dogs."J.V.M.S. 65. 109-112 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nishi, T., et al.: "Involvement of apoptosis in syncytial cell death induced by canine distemper virus."Comp.Immunol.Microbiol.Infect.Dis.. in press. (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nuntaprasert, A., et al.: "Expression and purification of recombinant swine interleukin-4"Comp.Immunol.Microbiol.Infect.Dis.. in press. (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nuntaprasert, A., et al.: "Characterization of specific antibodies and the establishment of sandwich ELISA and ELISPOT systems for swine IL-4"Comp.Immunol.Microbiol.Infect.Dis.. in press. (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tanaka, T., et al.: "Virological significance of low level hepatitis B virus infection in patients with hepatitis C liver disease."J.Med.Virol.. 72. 223-229 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tsukiyama-Kohara, K., et al.: "Activation of the CKI-cdk-Rb-E2F Pathway in Full-Genome Hepatitis C Virus Expressing Cells."J.Biol.Chem.. 279. 14531-14541 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yoneda, M. et al.: "Rinderpest virus phosphoprotein gene is major determinant of species-specific pathogenicity."Journal of Virology. in press. (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nuntaprasert, A., et al.: "The effect of recombinant swine interleukin-4 on swine immune cells and on pro- inflammatory cytokine productions in pigs."Comp.Immunol.Microbiol.Infect.Dis.. in press.. (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nuntaprasert, A. et al.: "Expression and characterization of the recombinant swine interleukin-6."Comp.Immunol.Microbiol.Infect.Dis.. in press.. (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nuntaprasert, A. et al.: "Establishment of swine interleukin-6 sandwich ELISA."Comp.Immunol.Microbiol.Infect.Dis.. in press.. (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yoneda, M. et al.: "Rinderpest virus H protein : role in determining host range in rabbits."J.Gen.Virol.. 83. 1457-1463 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 小原 恭子: "代謝および脂肪分化制御における4E-BP1の新たな役割"実験医学. 5 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 小原 恭子: "C型肝炎ウイルスによる細胞死抑制と病原性発現機序"特集●慢性肝炎と肝癌 Molecular Medicine. 7 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 小原 恭子: "4E-BP1遺伝子-翻訳抑制因子4E-BP1の代謝、および脂肪分化制御における役割 増刊「肥満症」"日本臨床. 5 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 小原 恭子: "C型肝炎動物モデルによる病態解明へのアプローチ"実験動物ニュース. 5 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 小原 恭子: "C型肝炎治療の新たな展開"WORLD FOCUS. 2 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yoneda, M., Bandyopadhyay, S., Shiotani, M., Fujita, K., Nuntaprasert, A., Miura, R., Baron, M.D., Barrett, T., Kai.C.: "Rinderpest virus H protein : role in determining host range in rabbits."J Gen Virol.. 83. 1457-1463 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kashiwagi, T., Hara, K., Kohara, M., Kohara, K., Iwahashi, J., Hamada, N., Yoshino, H., Toyoda, T.: "Kinetic analysis of C-terminally truncated RNA-dependent RNA polymerase of Hepatitis C virus."B.B.R.C. 290. 1188-1194 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hirama, K., Togashi, K., Wakasa, C., Yoneda, M., Nishi, T., Endo, Y., Miura, R., Tsukiyama-Kohara, K., Kai.C.: "Cytotoxic T -Lymphocyte Activity Specific for Hemagglutinin (H) Protein of Canine Distemper Virus in Dogs."JVMS. 65. 109-112 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nishi, T., Tsukiyama-Kohara, K., Togashi, K., Kohriyama, N., Kai, C.: "Involvement of apoptosis in syncytial cell death induced by canine distemper virus."Comp.Immunol.Microbiol.Infect.Dis.. (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nuntaprasert, A., Mori, Y, Fujita, K., Yoneda, M., Miura, R., Tsukiyama-Kohara, K., Kai, C.: "Expression and purification of recombinant swine interleukin-4."Comp.Immunol.Microbiol.Infect.Dis.. (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nuntaprasert, A., Mori, Y., Fujita, K., Yoneda, M., Miura, R., Tsukiyama-Kohara, K., Kai, C.: "Characterization of specific antibodies and the establishment of sandwich ELISA and ELISPOT systems for swine IL-4"Comp.Immunol.Microbiol.Infect.Dis.. (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tanaka, T., Inoue, K., Hayashi, Y., Abe, A., Tsukiyama-Kohara.K., Nuriya, H., Aoki, Y., Kawaguchi, R., Kubota, K., Yoshiba, M., Koike, M., Tanaka, S., Kohara.M.: "Virological significance of. low level hepatitis B virus infection in patients with hepatitis C liver disease."J.Med.Virol. 72. 223-229 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Tsukiyama-Kohara S.Tone', I.Maruyama, K.Inoue, A.Katsume, H.Nuriya, H.Ohmori, J.Ohkawa, K.Taira, Y.Hoshikawa, F.Shibasaki, M.Reth, Y.Minatogawa, M.Kohara: "Activation of the CKI-cdk-Rb-E2F Pathway in Full-Genome Hepatitis C Virus Expressing Cells."J.Biol.Chem. 279. 14531-14541 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yoneda, M., Miura, R., Barrett, T., Tsukiyama-Kohara, K, Kai C.: "Rinderpest virus phosphoprotein gene is major determinant of species-specific pathogenicity."J.Viorol.. (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nuntaprasert, A., Mori, Y., Muneta, Y., Yoshihara, K., Tsuikiyama-Kohara, K., Kai, C.: "The effect of recombinant swine interleukin-4 on swine immune cells and on pro-inflammatory cytokine productions in pigs."Comp.Immunol.Microbiol.Infect.Dis.. (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nuntaprasert, A., Mori, Y., Fujita, K., Yoneda, M., Miura, R., Tsuikiyama-Kohara, K., Kai, C.: "Expression and characterization of the recombinant swine interleukin-6."Comp.Immunol.Microbiol.Infect.Dis.. (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nuntaprasert, A., Mori, Y, Tuikiyama-Kohara, K., Kai C.: "Establishment of swine interleukin-6 sandwich ELISA."Comp.Immunol.Microbiol.Infect.Dis.. (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] "The novel role of 4E-BP1 on metabolism and differentiation of adiocytes."Experimental Medicine. 5 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] "4E-13P1 gene-The role of 4E-BP1 in the regulation of metabolism and adipose tissue differentiation-"Nippon Rinsho. 5 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] "Approach to characterize pathogenesis of hepatitis C virus using animal models."Experimental Animal News. 5 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hirama, K.et al.: "Lymphocyte Activity Specific for Hemagglutinin (H) Protein of Canine Distemper Virus in Dogs"J.V.M.S.. 65. 109-112 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Nishi, T., et al.: "Involvement of apoptosis in syncytial cell death induced by canine distemper virus"Comp.Immunol.Microbiol.Infect.Dis.. (in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Nuntaprasert, A., et al.: "Expression and purification of recombinant swine interleukin-4"Comp.Immunol.Microbiol.Infect.Dis.. (in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Nuntaprasert, A., et al.: "Characterization of specific antibodies and the establishment of sandwich ELISA and ELISPOT systems for swine IL-4"Comp.Immunol.Microbiol.Infect.Dis.. (in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Tanaka, T., et al.: "Virological significance of low level hepatitis B virus infection in patients with hepatitis C liver disease"J.Med.Virol.. (in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Tsukiyama-Kohara, K., et al.: "Activation of the CKI-cdk-Rb-E2F Pathway in Full-Genome Hepatitis C Virus Expressing Cells"J.Biol.Chem.. (in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yoneda, M.et al.: "Rinderpest virus phosphoprotein gene is major determinant of species-specific pathogenicity"Journal of Virology. (in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 小原 恭子: "4E-BP1遺伝子-翻訳抑制因子4E-BP1の代謝、および脂肪分化制御における役割"日本臨床 増刊「肥満症」. 5 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 小原 恭子: "C型肝炎動物モデルによる病態解明へのアプローチ"実験動物ニュース. 5 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yoneda, M., et al.: "Rinderpest virus H protein : role in determining host range in rabbits"J. Gen. Virol.. 83(Pt6). 1457-1463 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hirama, K. et al.: "Lymphocyte Activity Specific for Hemagglutinin (H) Protein of Canine Distemper Virus in Dogs"J. V. M. S.. 65. 109-112 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kashiwagi, T., et al.: "Kinetic analysis of C-terminally truncated ANA-dependent RNA polymerase of Hepatitis C virus"B. B. R. C.. 290. 1188-1194 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 小原 恭子: "代謝および脂肪分化制御における4E-BP1の新たな役割"実験医学. 4 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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