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Molecular epidemiology concerned with effect of nitric oxide synthase (NOS) gene polymorphism on NOS protein function in human cultured vascular endothelial cells

Research Project

Project/Area Number 14370123
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Hygiene
Research InstitutionNational University Corporation Tottori University

Principal Investigator

KISHIMOTO Takuji  Tottori University, Faculty of Medicine, Professor, 医学部, 教授 (00116394)

Co-Investigator(Kenkyū-buntansha) OSAKI Yoneatu  Tottori University, Faculty of Medicine, Associate Professor, 医学部, 助教授 (10325003)
OKAMOTO Mikizo  Tottori University, Faculty of Medicine, Assistant Professor, 医学部, 講師 (40032205)
KAETU Akihiko  Tottori University, Faculty of Medicine, Assistant, 医学部, 助手 (70248483)
Project Period (FY) 2002 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥13,600,000 (Direct Cost: ¥13,600,000)
Fiscal Year 2005: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2004: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2003: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2002: ¥4,600,000 (Direct Cost: ¥4,600,000)
Keywordsnitric oxide synthase / gene polymorphism / primary culture / endothelial cell / protein function / molecular epidemiology
Research Abstract

The relationship between cardiovascular disease and Glu298Asp polymorphism of the nitric oxide synthase (eNOS) gene in vascular endothelial cells is controversial due to conflicting results. To demonstrate the effects of Glu298Asp polymorphism of the eNOS gene on eNOS gene expression by excluding the effects of various confounding factors, eNOS mRNA and protein expressions were measured using cultured human vascular endothelial cells according to genotypes of Glu298Asp polymorphism of the eNOS gene. After human umbilical cords were aseptically obtained immediately after delivery, human umbilical vein endothelial cells (HUVECs) were collected by collagenase treatment, and HUVECs with the Glu298Glu or Glu298Asp genotype were obtained. eNOS mRNA expression was measured by real-time PCR/RT-PCR. eNOS protein expression was measured by an immunoassay method. HUVECs with Glu298Asp tended to show higher eNOS mRNA and protein expressions than those with Glu298Glu, but the difference was not significant. These results suggest that the genotypic difference of Glu298Glu and Glu298Asp has no effect on eNOS mRNA and protein expressions.

Report

(5 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (8 results)

All 2006 2005 2004

All Journal Article (8 results)

  • [Journal Article] Effects of Glu298Asp Polymorphism of Endothelial Nitric Oxide Synthase (eNOS) Gene on eNOS mRNA and Protein Expressions in Cultured Human Vascular Endothelial Cells2006

    • Author(s)
      Takuji Kishimoto, Akihiko Kaetsu, Yoneatsu Osaki, Mikizoh Okamoto, Mariya Nagai, Yoichi Kurosawa, Soichi Yoshida
    • Journal Title

      Yonago Acta medica 49

      Pages: 59-62

    • NAID

      110004600424

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Effects of Glu298Asp Polymorphism of Endothelial Nitric Oxide Synthase (eNOS) Gene on eNOS mRNA and Protein Expressions in Cultured Human Vascular Endothelial Cells2006

    • Author(s)
      Takuji Kishimoto, et al.
    • Journal Title

      Yonago Acta medica 49

      Pages: 59-62

    • NAID

      110004600424

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Effects of Glu298Asp polymorphism of Endothelial Nitric Oxide Synthase (eNOS) Gene on eNOS mRNA and Protein Expressions in Cultured Human Vascular Endothelial Cells2005

    • Author(s)
      Takuji Kishimoto, et al.
    • Journal Title

      Yonago Acta medica 49

      Pages: 59-62

    • NAID

      110004600424

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] eNOS Glu298Asp polymorphism and hypertension in a cohort study in Japanese2004

    • Author(s)
      Takuji Kishimoto, Yumiko Misawa, Akihiko Kaetu, et al.
    • Journal Title

      Preventive Medicine 39

      Pages: 927-931

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] The lack of relationship between an endothelin-1 gene polymorphism (Ala288Ser) and incidence of hypertension : A retrospective cohort study among Japanese workers.2004

    • Author(s)
      Akihiko Kaetu, Takuji Kishimoto, Yoneatu Osaki, et al.
    • Journal Title

      Journal of Epidemiology 114

      Pages: 129-136

    • NAID

      10013439321

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] eNOS Glu298Asp polymorphism and hypertension in a cohort study in Japanese2004

    • Author(s)
      Takuji Kishimoto, Yumiko Misawa, Akihiko Kaetu, Mariya Nagai, Yoneatsu Osaki, Mikizoh Okamoto, Soiti Yoshida, Yoichi Kurosawa, Soji Fukumoto
    • Journal Title

      Preventive Medicine 3

      Pages: 927-931

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The lack of relationship between an endothelin-1 Gene polymorphism (Ala288Ser) and incidence of hypertension A retrospective cohort study among Japanese workers2004

    • Author(s)
      Akihiko Kaetu, Takuji Kishimoto, Yoneatu Osaki, Mikizoh Okamoto, Soji Fukumoto, Yoichi Kurosawa
    • Journal Title

      Journal of Epidemiology 14

      Pages: 129-136

    • NAID

      10013439321

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The lack of relationship between an endothelin-1 gene polymorphism (Ala288Ser) and incidence of hypertension : A retrospective cohort study among Japanese workers.2004

    • Author(s)
      Akihiko Kaetu, Takuji Kishimoto, Yoneatu Osaki, et al.
    • Journal Title

      Journal of Epidemiology 14

      Pages: 129-136

    • NAID

      10013439321

    • Related Report
      2004 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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