| Project/Area Number |
14370271
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Radiation science
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| Research Institution | International University of Health and Welfare (2003)
The University of Tokyo (2002) |
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Principal Investigator |
SUZUKI Norio International University of Health and Welfare, Radiological Sciences, Professor, 保健学部, 教授 (10010050)
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| Co-Investigator(Kenkyū-buntansha) |
MORITA Akinori The University of Tokyo, Radiation Research, Assistant professor, 大学院・医学系研究科, 助手 (90334234)
ENOMOTO Atsushi The University of Tokyo, Radiation Research, Assistant professor, 大学院・医学系研究科, 助手 (20323602)
MATSUMOTO Yoshihisa The University of Tokyo, Radiation Research, Assistant professor, 大学院・医学系研究科, 助手 (20302672)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥12,800,000 (Direct Cost: ¥12,800,000)
Fiscal Year 2003: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2002: ¥9,400,000 (Direct Cost: ¥9,400,000)
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| Keywords | SAPK / JNK / wortmannin / hyperthermia / cell death / apoptosis / DNA-PK / Heat sensitization / U937 cells / p53 / 温熱 / シグナル伝達 / p53 null / U937細胞 / 細胞死、アポトーシス / 温熱高感受性 / Akt / Wortmannin / リン酸化特異抗体 / 細胞死 / セラミド / 温熱療法 |
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| Research Abstract |
The overall goal of the present project has been to analyze mechanisms of heat-induced apoptotic cell death and radio-sensitization for better treatment of cancer. Toward this goal, we have accomplished the following progress.
1)We have clarified the involvement of ceramide-SAP/JNK pathway, and radio-sensitization process in heat-induced apoptotic cell death.
2)RT-PCR analysis of apoptosis-related genes revealed that the expression of c-myc gene was reduced in the process of cell death. The introduction of c-myc antisense oligonucleotides or the treatment of c-Myc inhibitor induced cell death.
3)Involvement and the role of DNA-PK in radio-sensitization by heat was evaluated using various repair deficient and knockout mutants of mammalian cells and DT40 chicken cells.
4)We found appearance of fragment of JNK, which were identified as cleaved products by Caspase 3 from INK.
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