| Project/Area Number |
14370351
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Nagoya University |
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Principal Investigator |
NAKAO Akimasa Nagoya University, Graduate School of Medicine, Professor, 大学院・医学系研究科, 教授 (70167542)
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| Co-Investigator(Kenkyū-buntansha) |
MIZUNO Masaaki Nagoya University, Graduate School of Medicine, Associate Professor, 大学院・医学系研究科, 助教授 (70283439)
TACHIBANA Katsuro Fukuoka University, School of Medicine, Assistant Professor, 医学部, 講師 (40271605)
SUZUKI Haruhiko Nagoya University, Graduate School of Medicine, Associate Professor, 大学院・医学系研究科, 助教授 (90283431)
YOSHIKAWA Kazuhiro Aichi Medical University, School of Medicine, Assistant Professor, 医学部, 講師 (60109759)
YOSHIOKA Kentaro Nagoya University, University Hospital, Assistant Professor, 医学部附属病院, 講師 (60201852)
福田 吉秀 名古屋大学, 医学部附属病院, 講師 (40181284)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥13,900,000 (Direct Cost: ¥13,900,000)
Fiscal Year 2003: ¥6,700,000 (Direct Cost: ¥6,700,000)
Fiscal Year 2002: ¥7,200,000 (Direct Cost: ¥7,200,000)
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| Keywords | space medicine / remote control / gene therapy / regeneration / liver |
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| Research Abstract |
Many kinds of gene therapy project have been tried for various diseases such as congenital metabolic ones, cardiovascular ones, and malignancies all over the world, however, the effectiveness has not been clearly identified due to the immature development of gene transferred vector, In this study, we investigated the effect of space-mediated and remote-controlled method in liver-directed gene therapy as a novel strategy. The ultrasound-mediated gene transfer with echo-contrast agents has been performed to analyze the transduction efficiency by LacZ or EGFP cDNA and to measure the anti-tumor effect by IFNbeta cDNA, In addition, using the hepatic metastasis model of murine colon cancer and the subcutaneous xenograft model of human hepatoma cells, in vivo anti-tumor effect of IFNbeta gene therapy by ultrasound with echo-contrast agent was examined. The results demonstrated that the ultrasound-mediated gene therapy with echo-contrast agents was applicable for hepatic cancer treatment. Next we investigated the effect of hyperbaric oxygen (HBO)-mediated gene transfer to the cold-preserved donor organ in the transplantation. Under the low temperature and short transfection period, the transgene expression by adneovirus-mediated gene transfer was significantly lower than that under the normal condition. The addition of HBO in the transfection period augmented the transgene expression compared to the simple method. We investigated the basic study about the remote-controlled gene therapy using micro and nanorobotics. The results demonstrated that the cavitation effect by the ultrasound was feasible for the remote-controlled gene therapy using micro and nanorobotics. It is concluded that the space-mediated and remote-controlled method are hopeful strategies in liver-directed gene therapy.
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