New immunosuppressive reagent FTY720 and its combined immunotherapy with anti-ICOS antibody to organ transplantation

• Project/Area Number: 14370368
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General surgery
• Research Institution: National Research Institute for Child Health and Development
• Principal Investigator: KIMURA Hiromitsu National Research Institute for Child Health and Development, Division of Collaborative Research, The head of a laboratory (80115477)
• Co-Investigator(Kenkyū-buntansha): 岩崎 師壽江 国立成育医療センター研究所, 共同研究管理室, 流動研究員
• Project Period (FY): 2002 – 2005
• Project Status: Completed (Fiscal Year 2005)
• Budget Amount: ¥12,300,000 (Direct Cost: ¥12,300,000); Fiscal Year 2005: ¥2,400,000 (Direct Cost: ¥2,400,000); Fiscal Year 2004: ¥3,100,000 (Direct Cost: ¥3,100,000); Fiscal Year 2003: ¥3,100,000 (Direct Cost: ¥3,100,000); Fiscal Year 2002: ¥3,700,000 (Direct Cost: ¥3,700,000)
• Keywords: Immunosuppressant / FTY720 / ICOS / Microchimerism / Quantitative RT-PCR / 抗ICOS抗体 / AdCTLA4-Ig / CD28ファミリー / 慢性拒絶反応 / 心臓移植 / 皮膚移植 / CD152 / sphingosine 1-phosphate receptors / 免疫学的メモリーT細胞 / 樹状細胞 / 遺伝子ベクター / 第三世代HIV改変lenti virusベクター / 移植免疫寛容 / CTLA-4Ig / メモリーT細胞 / 長期リンパ液排導 / TDL / 再循環 / 臓器移植 / 移植免疫 / 免疫寛容

Research Abstract

New immunosuppressive reagent FYY720, in particular, its effect on immunological memory was investigated. Employing sex-mismatched skin isograft model, FTY720 was found to be no serious effect on immunological memory despite of strong immunosuppressive activity.
As for co-stimulation molecule of CD28 superfamily, i.e., CTLA-4 and ICOS, was investigated in the preset study. Although single immunotherapy of Adenovirus vector encoding chimeric molecule of CTLA-4 and IgG (Ad-CTLA-4-IgG) and anti-ICOS monoclonal antibody had limited activity to rat cardiac allograft model. Thus cardiac allografts were ultimately rejected in chronic fashion under Ad-CfLA-4-IgG or anti-ICOS immunotherpy. In particular anti-ICOS single immunotherapy had less effective than CTLA4-IgG However, when the imuunotherapy of Ad-CTLA-4-IgG and anti-ICOS were combined, no rejection was observed. Moreover, immunohistological examination revealed that the combined Ad-CTLA-4-IgG and anti-ICOS immunotherapy were found to induce more complete tolerance than single immunotherapy. Nevertheless the combined immunotherapy failed to induce complete tolerance to skin allograft models. These results indicate that regulation of T cell activation co-stimulation molecules, in particular, CD28 superfamily, are apparent targets of imuunosuppression strategy.
In order to induce complete tolerance to skin allograft models, more precise study must be explored.

Research Products

• [Journal Article] Potential Reulatory Function of Dendritic Cells Overexpression Indoleamine 2, 3-Dioxygenase (2005)
  • Author(s): Funeshima N, Fujino M, Kitazawa Y, Hara Y, Hayakawa K, Okuyama T, Kimura H, LI XK.
  • Journal Title: J Gene Medicine May ; 7(5) Pages: 565-75
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Studies on the most efficient vector systems for gene transduction into dendritic cells. (2005)
  • Author(s): Satoh E, Yan H, Miyagi T, Li XK, Sugiura W, Yamamoto N, Teramoto K, Arii S, Kimura H.
  • Journal Title: Transplant Proc. Jan-Feb ; 37(1 Pages: 12-4
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Phenotype and functional identity of GM -CSF-independent dendritic cells generated by long -term propagation of DC progenitor cells in bone marrow cells and skin Langerhans cells. (2005)
  • Author(s): Katayama H, Hattori Y, Ogata K, Yan H, Satoh E, Teramoto K, Arii S, Kamide R, Nakagawa H, Kimura H.
  • Journal Title: Transplant Proc. Jan-Feb ; 37(l Pages: 17-9
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Comparison of the vector systems for gene transduction into ratdendritic cells and peritoneal exudate cells. (2005)
  • Author(s): Satoh E, Hara Y, Fuji N, Li XK, Teramoto K, Arii S. Kimura H.
  • Journal Title: Transplant Proc. May ; 37(4) Pages: 1953-6
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Li XK. Inhibition of allogeneic T-cell responses by dendritic cells expressing transduced indoleamine 2,3-dioxygenase. (2005)
  • Author(s): Funeshima N, Fujino M, Kitazawa Y, Hara Y, Hayakawa K, Okuyama T, Kimura H.
  • Journal Title: J Gene Medicine 7(5) Pages: 565-75
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Studies on the most efficient vector systems for gene transduction into dendritic cells (2005)
  • Author(s): Satoh E, Yan H, Miyagi T, Ii XK, Sugiura W, Yamamoto N, Teramoto K, Arii S, Kimura H
  • Journal Title: Transplant Proc 37(1) Pages: 12-12
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Phenotype and functional identity of GM-CSF-independent dendritic cells generated by long-term propagation of DC progenitor cells in bone marrow cells and skin Langerhans cells. (2005)
  • Author(s): Katayama H, Hattori Y, Ogata K, Yan H, Satoh E, Teramoto K, Arii S, Kamide R, Nakagawa H, Kimura H
  • Journal Title: Transplant Proc. 37(1) Pages: 17-9
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Comparison of the vector systems for gene transduction into rat dendritic cells and peritoneal exudate cells. (2005)
  • Author(s): Satoh E, Hara Y, Fuji N, Li XK, Teramoto K, Arii S. Kimura H.
  • Journal Title: Transplant Proc. 37(4) Pages: 1953-6
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Potential Regulatory Function of Dendritic Cells Overexpression Indoleamine 2,3-Dioxygenase (2005)
  • Author(s): Fuuneshima N, Fujino M, Kitazawa Y, Hara Y, Hayakawa K, Okuyama T, Kimura H, Li XK.
  • Journal Title: J Gene Medicine May;7(5) Pages: 565-575
• [Journal Article] Studies on the most efficient vector systems for gene transduction into dendritic cells. (2005)
  • Author(s): Satoh E, Yan H, Miyagi T, Li XK, Sugiura W, Yamamoto N, Teramoto K, Arii S, Kimura H.
  • Journal Title: Transplant Proc. Jan-Feb;37(1) Pages: 12-14
• [Journal Article] Phenotype and functional identity of GM-CSF-independent dendritic cells generated by long-term propagation of DC progenitor cells in bone marrow cells and skin Langerhans cells. (2005)
  • Author(s): Katayama H, Hattori Y, Ogata K, Yan H, Satoh E, Teramoto K, Arii S, Kamide R, Nakagawa H, Kimura H.
  • Journal Title: Transplant Proc. Jan-Feb;37(1) Pages: 17-19
• [Journal Article] Comparison of the vector systems for gene transduction into rat dendritic cells and peritoneal exudate cells. (2005)
  • Author(s): Satoh E, Hara Y, Fuji N, Li XK, Teramoto K, Arii S, Kimura H.
  • Journal Title: Transplant Proc. May;37(4) Pages: 1953-1956
• [Journal Article] AdCTLA-4Ig combined with donor splenocytes, bone marrow cells and anti-ICOS antibody treatment induce tolerance in a rat heart transplantation model (2004)
  • Author(s): Guo L, Fujino M, Kimura H, Funeshima N, Kitazawa Y, Harihara Y, Tezuka K, Makuuchi M, Suzuki S, LiXK.
  • Journal Title: 1Transplantation Internation Jan ; 17(1) Pages: 15-21
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Establishment of a set of combined immunodeficient DA/Slc-Foxnl (rnu) Lyst (bg) congenic rat strains. (2004)
  • Author(s): Masui N, Nishikawa T, Takagi Y, Kimura H, Mori M, Yokose S, Asai H, Gonda T, Yanabe M, Sato K.
  • Journal Title: Exp Anim. 53(5) Pages: 399-407
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Phenotype and function of GM-CSF independent dendritic cells generated by long-term propagation of rat bone marrow cells. (2004)
  • Author(s): Nan H, Miyagi T, Satoh E, Sugiura W, Yamamoto N, Kimura H.
  • Journal Title: Cell Immunol 229(2) Pages: 117-29
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Significant enhancement byanti-ICOS antibody of suboptimaltacrolimus immunosuppression in rat liver transplantation. (2004)
  • Author(s): Guo L, Li XK, Enosawa S, Funeshima N, Suzuki S, Kimura H, Sugawara Y, Tezuka K, Makuuchi M.
  • Journal Title: Liver Transpl June ; 10(6) Pages: 743-7
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Differences in lymphocyte gene expression between tolerant and syngeneic liver grafted rats (2004)
  • Author(s): Fujino M, Kitazawa Y, Kawasaki M, Funeshima N, Kimura H, Nakajima T, Saito H, Li XK.
  • Journal Title: Liver Transpl 2004Mar ; 10(3) Pages: 379-91
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Establishment of a set of combined immunodeficient DA/Slc-Foxnl(rnu) LystQbg) congenic rat strains (2004)
  • Author(s): Masui N, Nishikawa T, Takagi Y, Kimura H. MoriM, Yokose S, Asai H, Gonda T, Yanabe M, Sato K
  • Journal Title: Exp Anim 53(5) Pages: 399-407
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Phenotype and function of GM-CSF independent dendritic cells generated bv lone-term nrooaeation of rat bone marrow cells. (2004)
  • Author(s): Yan H, Miyagi T, Satoh E, Sugiura W, Yamamoto N, Kimura H.
  • Journal Title: Cell Immunol. 229(2) Pages: 117-29
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Significant enhancement by anti-ICOS antibody of suboptimal tacrolimus immunosuppression in rat liver transplantation. liver Transpl (2004)
  • Author(s): Guo L, Li XK, Enosawa S, Funeshima N, Suzuki S, Kimura H. Sugawara Y. Tezuka K. Makuuchi M.
  • Journal Title: Liver Transpl. 10(6) Pages: 743-7
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Li XK. Differences in lymphocyte gene expression between tolerant and syngeneic liver grafted rats. (2004)
  • Author(s): Fujino M, Kitazawa Y, Kawasaki M, Funeshima N, Rimura H. Nakajima T Saito H
  • Journal Title: Liver Transpl 10(3) Pages: 379-91
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Establishment of a set of combined immunodeficient DA/Slc-Foxn1(rnu) Lyst(bg) congenic rat strains. (2004)
  • Author(s): Masui N, Nishikawa T, Takagi Y, Kimura H, Mori M, Yokose S, Asai H, Gonda T, Yanabe M, Sato K.
  • Journal Title: Exp Anim. 53(5) Pages: 399-407
• [Journal Article] Phenotype and function of GM-CSF independent dendritic cells generated by long-term propagation of rat bone marrow cells. (2004)
  • Author(s): Yan H, Miyagi T, Satoh E, Sugiura W, Yamamoto N, Kimura H.
  • Journal Title: Cell Immunol 229(2) Pages: 117-129
• [Journal Article] Significant enhancement by anti-ICOS antibody of suboptimal tacrolimus immunosuppression in rat liver transplantation. (2004)
  • Author(s): Guo L, Li XK, Enosawa S, Funeshima N, Suzuki S, Kimura H, Sugawara Y, Tezuka K, Makuuchi M.
  • Journal Title: Liver Transpl June;10(6) Pages: 743-747
• [Journal Article] Differences in lymphocyte gene expression between tolerant and syngeneic liver grafted rats. (2004)
  • Author(s): Fujino M, Kitazawa Y, Kawasaki M, Funeshima N, Kimura H, Nakajima T, Saito H, Li XK.
  • Journal Title: Liver Transpl Mar;10(3) Pages: 379-391
• [Journal Article] Ameliolation of experimental autoimmune encephalomyelitis in Lewisrats by FTY 720 (2003)
  • Author(s): Fujino M, Funeshima, N, Kitazawa Y, Kimura H, Amemiya H, Suzuki S, Li X-K
  • Journal Title: The Journal of Pharmacology and Experimental Therapeutics J Pharmacol Exp Ther. Apr ; 305(1) Pages: 70-7
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] MProlonged survival of liver xenograft with AdCTLA-4Ig one dose tailvein injection (2003)
  • Author(s): Guo L, xiao-Kang Li, Harihara Y, Fujino M, Funeshima N, Kimura H, Amemiya H, Suzuki S, Makuuchi M
  • Journal Title: Transplant Immunology Apr-Jun11(2 Pages: 155-62
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] HFurther studies on thermal treatment of two -cell stage embryos to produce complete ES -cell derived mice by cell -aggregation methods (2003)
  • Author(s): Iwasaki S, Kimura H
  • Journal Title: methodsDev Growth Differ 45(5-6) Pages: 427-34
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Simultaneous blockade of co-stimulatory signals, CD28 and ICOS, induced a stable tolerance in rat heart transplantati on (2003)
  • Author(s): Guo L, Fujino M, Kimura H, Funeshima N, Kitazawa Y, Harihara Y, Tezuka K, Makuuchi M, Suzuki S, Li XK
  • Journal Title: TranspIantImmunology 12 Pages: 41-48
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Prolongation of liver xenograft survival by adenovirus vector -mediated CTLA-4Ig gene transfer (2003)
  • Author(s): Guo L, Li X, Enosawa S, Harihara Y, Funeshima N, Kimura H, Fujino M, Makuuchi M, Suzuki S.
  • Journal Title: Transplant Immunology 11 Pages: 155-162
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Li X-KAmeliolation of experimental autoimmune encephalomyelitis in Lewis rats by FTY 720 (2003)
  • Author(s): Fujino M, Funeshima, N, Kitazawa Y, Kimura H, Amemiya H, Suzuki S
  • Journal Title: The Journal of Pharmacology and Experimental Therapeutics J Pharmacol Exp Ther 305(1) Pages: 70-7
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] MProlonged survival of liver xenograft with AdCTLA-4Ig one dose tail vein injection (2003)
  • Author(s): Guo L, Xiao-Kang Li, Harihara Y, Fujino M, Funeshima N, Kimura H. Amemiya H, Suzuki S, Makuuchi
  • Journal Title: Transplant Immunology 11(2) Pages: 155-62
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Further studies on thermal treatment of two-cell stage embryos to produce complete ES-cell derived mice by cell-aggregation (2003)
  • Author(s): Iwasaki S, Kimura H
  • Journal Title: Growth Differ 45(5-6) Pages: 427-34
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Li XK. Simultaneous blockade of co-stimulatory signals, CD28 and ICOS, induced a stable tolerance in rat heart transplantation (2003)
  • Author(s): Guo L, Fujino M, Kimura H. Funeshima N, Kitazawa Y, Harihara Y, Tezuka K, Makuuchi M, Suzuki S
  • Journal Title: TransplantImmunology 12 Pages: 41-48
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Prolongation of liver xenograft survival by Adenovirus vector-mediated CTLA-4Ig gene transfer (2003)
  • Author(s): Guo L, Li X, Enosawa S, Harihara Y, Funeshima N, Kimura H. Fujino M, Makuuchi M, Suzuki S
  • Journal Title: Transplant Immunology 11 Pages: 155-162
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] SProlonged survival of rat hepatic allograft with mouse monoclonalantibody against inducible co-stimulator (ICOS) (2002)
  • Author(s): Guo L, Xiao-Kang Li, Funeshima N, Fujino M, Nagata Y, Kimura H, Amemiya H, Enosawa S, Harihara, Y, Makuuchi M, Suzuki
  • Journal Title: Transplantatio Apr 15 ; 73(7) Pages: 1027-32
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] SProlonged survival of rat hepatic allograft with mouse monoclonal antibody against inducible co-stimulator (ICOS) (2002)
  • Author(s): Guo L, Xiao-Kang Li, Funeshima N, Fujino M, Nagata Y, Kimura H. Amemiya H, Enosawa S, Harihara, Y, Makuuchi M, Suzuki
  • Journal Title: Transplantation 73(7) Pages: 1027-32
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Li XK. AdCTLA-4Ig combined with donor splenocytes, bone marrow cells and anti-ICOS antibody treatment induce tolerance in a rat heart transplantation model
  • Author(s): Guo L, Fujino M, Kimura H. Funeshima N, Kitazawa Y, Harihara Y, Tezuka K, Makuuchi M, Suzuki S
  • Journal Title: Transplantation Internationl 17(1) Pages: 15-21
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Inhibition of allogeneic T-cell responses by dendritic cells expressing transduced indoleamine 2,3-dioxygenase.
  • Author(s): Funeshima N, Fujino M, Kitazawa Y, Hara Y, Hayakawa K, Okuyama T, Kimura H, Li XK.
  • Journal Title: J Gene Medicine (In press)
• [Presentation] Determination Of The Most Efficient Vector Systems For Transduction Into Dendritic Cells(DC) (2005)
  • Author(s): Eigo Satoh, Hua Yan, Tohko Miyagi, Xiao-Kang Li, Wataru Sugiura, Naoki Yamamoto, Kenichi Teramoto, Shigeki Arii, Hiromitsu Kimura
  • Organizer: 8^<th> Annual meeting of American Society of Gene Therapy
  • Place of Presentation: St. Louis(USA)
  • Year and Date: 2005-06-01
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] DETERMINATION OF THE MOST EFFICIENT VECTOR SYSTEMS FOR GENE TRANSDUCTION INTO DENDRITIC CELLS (DC) (2004)
  • Author(s): Eigo Satoh, Hua Yan, Tohko Miyagi, Xiao-Kang Li, Wataru Sugiura, Naoki Yamamoto, Kenichi Teramoto, Shigeki Arii, Hiromitsu Kimura
  • Organizer: 7^<th>Annual meeting of American Society of Gene Therapy
  • Place of Presentation: Minneapolis (USA)
  • Year and Date: 2004-06-04
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] STUDIES ON THE MOST EFFICIENT VECTOR SYSTEMS FOR GENE TRANSDUCTION INTO DENDRITIC CELLS (DC) (2004)
  • Author(s): Eigo Satoh, Hua Yan, Tohko Miyagi, Xiao-Kang Li, Wataru Sugiura, Naoki Yamamoto, Kenichi Teramoto, Shigeki Arii, Hiromitsu Kimura
  • Organizer: XX In ternational Congress of the Transplantation Society
  • Place of Presentation: Vienna (Austria)
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Xiao-kang Li Potential Regulatory Function of Dendritic Cells Overexpression Indoleamine 2,3-Dioxygenase (2004)
  • Author(s): Naoko Fuji-Funeshima, Yoshiaki Hara, Tuhsuke Kitazawa, Kensuke Adachi, Hiromitsu Kimura
  • Organizer: 3^<rd> International Congress on Immunosuppuression
  • Place of Presentation: San Diego (USA)
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] EVALUATION OF THE MOST EFFICIENT VECTOR SYSTEMS FOR GENETRANSDUCTION INTO DENDRITIC CELLS (DC) (2004)
  • Author(s): Eigo Satoh, Naoko Fuji, Xiao-Kang Li, Kenichi Teramoto, Shigeki Arii, Hiromitsu Kimura
  • Organizer: 3^<rd>International Congress on Immunosuppuression
  • Place of Presentation: San Diego (USA)
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] IMMUNOSUPPRESSIVE EFFECT OF LONG TERM DRAINAGE OF THORACIC DUCT ON MMUNOLOGICAL MEMORY IN ADULT THYME CTOMIZED RATS (2004)
  • Author(s): Eigo Satoh, Yuhichi Hattori, Naoko Fuji, Xiao-Kang Li, Kenichi Teramoto, Shipeki Arii. Hiromitsu Kimura
  • Organizer: 3^<rd> International Congress on Immunosuppuression
  • Place of Presentation: San Diego (USA)
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Immunological Behavior of Enhanced Green Fluorescence Protein (EGFP) and Beta Galactosidase (B-Gal) Gene Product : I. Effect of Minor Histocompatibility Antigen (2003)
  • Author(s): Eigo Satoh, Yasuo Kokai, Kenichi Teramoto, Shigeki Arii, Hiromitu Kimura
  • Organizer: Transplant Congress 2003
  • Place of Presentation: Washington, D.C.
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Cytokine Requirement forthe Generation of a Large Number of Rat Dendritic Cells (DC) by In Vitro Culturing of Bone Marrow Cells And Its Selection of CD 161 (NKR-P1 A) (2003)
  • Author(s): Eigo Satoh, Hua Yan, Xiao-K. Li, Kenichi Teramoto, Shigeki Arii, Hiromitu Kimura
  • Organizer: 12 th International Symposium on Molecular Cell Biology of Macrophage 2003
  • Place of Presentation: Utunomiya
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] GENERATION OF GM-CSF INDEPENDENT MYELOID DENDRITIC CELLS(DC) FROM BONE MARROW CELLS (BMC) IN GM-CSF RECEPTOR DEFICIENT MOUSE (2003)
  • Author(s): Eigo Satoh, Hua Yan, Robb L, Hajime Hikino, Tohko Miyagi, Hirohisa Saito, Daniel P. Gold, Xiao-K. Li, Kenichi Teramoto, Shigeki Arii, Hiromitsu Kimura
  • Organizer: 8 th Congress of the Asian Society of Transplantation
  • Place of Presentation: Kuala Lumpur
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] CYTOKINE REQUIREMENT FOR THE GENERATION OF A LARGE NUMBER OF RAT DENDRITICCELLS (DC) BY IN VITRO CULTURING OF BONE MARROW CELLS AND IT S SELEC-TION OF CD 161 (NKR-P 1 A) (2003)
  • Author(s): Eigo Satoh, Hua Yan, Tohko Miyagi, Hirohisa Saito, Daniel P. Gold, Xiao-K. Li, Masayuki Fujino, Kenichi Teramoto, Shigeki Arii, Hiromitsu Kimura
  • Organizer: 8 th Congress of the Asian Society of Transplantation
  • Place of Presentation: Kuala Lumpur
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] TRANSGENIC ENHANCED GREEN FLUORESCENCE PROTEIN (EGFP) AND BETA GALACTO-SIDASE (B-GAL) GENES BEHAVEAS MINOR HISTOCOMPATIBILITY ANTIGENS (2003)
  • Author(s): Eigo Satoh, Yasuo Kokai, Kenichi Teramoto, Shigeki Arii, Hiromitsu Kimura
  • Organizer: 8 th Congress of the Asian Society of Transplantation
  • Place of Presentation: Kuala Lumpur
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] CYTOKINE REQUIREMENT FOR THE GM-CSF INDEPENDENT DEVELOPMENT OF HUMAN DENDRITIC CELLS (DC) BY IN VITRO CULTURING OF HEMATOPOIETC STEM CELL. (2003)
  • Author(s): Eigo Satoh, Xiao-K. Li, Yan Hua, Tohko Miyagi, Hirohisa Saito, Daniel P. Gold, Masayuki Fujino, Taga Tetsuya, Hiroshi Amemiya, Seiichi Suzuki, Kenichi Teramoto, Shigeki Arii, Hiromitsu Kimura
  • Organizer: 8 th Congress of the Asian Society of Transplantation
  • Place of Presentation: Kuala Lumpur
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] EFFECT OF LONG TERM DRAINAGE OF THORACIC DUCT ON IMMUNOLOGICALT CELL MEMORY IN ADULT THYMECTOMIZED RATS (2003)
  • Author(s): Yuhichi Hattori, Eigo Satoh, Hua Yan, Tohko Miyagi, Xiao-Kang Li, Wataru Sugiura, Naoki Yamamoto, Kenichi Teramoto, Shigeki Arii, Hiromitsu Kimura
  • Organizer: 8 th Congress of the Asian Society of Transplantation
  • Place of Presentation: Kuala Lumpur
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Differential Expression of CD4 in GM-CSF Independent Human Dendritic Cells (DC) from CD34+ and CD 133+ Umbilical Cord Blood Cells (UBC) (2002)
  • Author(s): Hua Yan, Xiao-Kang Li, Tetsuo Taga, Hiroshi Amemiya, Seiichi Suzuki, Hirohisa Saito, Hiromitsu Kimura
  • Organizer: 7^<th> International Symposium on Dendritic Cells, Bamberg
  • Place of Presentation: Bamberg, Germany
  • Description: 「研究成果報告書概要(欧文)」より
• [Book] 免疫寛容とギメリズム-過去、現在、未来-臨床免疫学(上)臨床編III. 移植免疫 (2005)
  • Author(s): 木村廣光
  • Publisher: 日本臨床 : 63巻増刊号4
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Hua Yan, Tohko Miyagi, Eigo Satoh, et al.: "Phenotype and Function of GM-CSF Independent Dendritic Cells Generated by Long-Term Propagation of Rat Bone Marrow Cells"Cellular Immunology. in press.
• [Publications] Shizue Iwasaki, Hiromitsu Kimura: "Further Studies on thermal treatment of two-stage embyos to produce complete embryonic sstem-cell-derived micce by cell-aggregation methods"Development, Grwoth and Differentiation. 45. 427-434 (2003)
• [Publications] Lei Guo, Masayuki Fujino, Hiromitsu Kimura, et al.: "Simultaneous blockage of co-stimulatory signals, CD 28 and ICOS, induced a stable tolerance in rat heart transplantation"Transplant Immunology. 12. 41-48 (2003)
• [Publications] Lei Guo, Masayuki Fujino, Hiromitsu Kimura, et al.: "AdCTLA-4Ig combined with donor splenocytes, bone marrow cells and anti-ICOS antibody treatment induce tolerance in a rat heart transplantation model"Transplantation International. 17. 15-21 (2003)
• [Publications] Hayakawa-K, Guo-L, Terenyeva-EA et al.: "Size-exclusion chromatography of biological samples which contain extreamly alkaline proteins"Journal of Biochem Biophys Methods. 56. 155-162 (2003)
• [Publications] Lei Guo, Masayuki Fujino, Hiromitsu Kimura, et al.: "Prolongation of liver xenograft survival by adenovirus vector mediated CTLA-Ig gene transfer"Transplant Immunology. 11. 155-162 (2003)
• [Publications] L.Guo, et al.: "Simultaneous Blockade of Co-stimulatory Signals, CD28 and ICOS, Induced a Stable Tolerance in Rat Heart Transplantation"Transplant Immunology. (In press).
• [Publications] L.Guo, et al.: "Prolonged survival in rat liver transplantation woth mouse monoclonal antibody against an inducible co-stimulator (ICOS)"Transplantation. 73. 1027-1032 (2002)