| Co-Investigator(Kenkyū-buntansha) |
EBATA Naoyuki Shindo Faiber Co.Ltd., R & D Division, 研究開発本部, 開発主任
MORI Yuuichi Waseda Univerisity, Advanced Research Center for Science and Engineering, 理工学総合研究センター, 教授 (10288003)
KOSUGE Takayuki Yokohama City University, University Hospital, Assistant, 医学部附属病院, 助手 (10336555)
市川 由起夫 横浜市立大学, 医学部, 助手 (70305473)
磯田 晋 横浜市立大学, 医学部附属病院, 講師 (40254172)
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| Budget Amount *help |
¥15,100,000 (Direct Cost: ¥15,100,000)
Fiscal Year 2003: ¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥11,500,000 (Direct Cost: ¥11,500,000)
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| Research Abstract |
A fabric vascular prosthesis coated with bioabsorbable mateiraal was developed. This project was aimed to obtain a coated fabric vascular prosthesis free from bovine spongious encepharopathy. At first, we searched a suitable material for the coating. After some examinations, we selected non-pathological protein free substances, such as cellulose, extremely high molecule hyaluronic acid, polyethylene glycol, polyvinyl alcohol, and their substrates. Our basic experiments showed that the most suitable material for this purpose was carbomethyl cellulose (CMC). Secondly, we made efforts was how to insolubilize the materials on a fabric prosthesis. We checked chemical reagents such as formaldehyde, glutarardehyde and hexamethylene diisocyanate. All of them showed cytotoxicity. After long-term washing, the materials crosslinked with these chemical reagents showed cytotoxicity. From this results, we gave up to use these cehmical reagent.
Finally, we adpoted physical methos such as gannma-ray irradiation, ultraviolet irradiation., and electron beam irradiation. But all of them showed adequate damages to the fabric fibers.
Therefore, we used heat-cross-linking method. This was a simple method, but it did not show any citotoxic reacton. Thirdly, we made effort to create a best matrilx of the fabric vascular prosthesis for the coating.. For this purpose, we developed a prosthesis fabricated with ultrafine polyester fibers. These ultrafine fibers can trap the coated substance very firmaly. Finally, with these basic experiments, a fabric prosthesis made of ultrafine polyester fibers which was coated with CMC. The CMC was insolubilized by heat cross-linking. We implanted the created prostheses into the thoracic descending aortae of dogs. The graft showed expected results. We are now underway to observe this in vivo experiment for long-term analysis.
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