| Project/Area Number |
14370433
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | KAGAWA UNIVERSITY(FACULTY OF MEDICINE) (2003)
Okayama University (2002) |
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Principal Investigator |
TAMIYA Takashi KAGAWA UNIVERSITY, SCHOOL OF MEDICINE, ASSOCIATE PROFESSOR, 医学部, 助教授 (50252953)
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| Co-Investigator(Kenkyū-buntansha) |
ONO Yasuhiro OKAYAMA UNIVERSITY, SCHOOL OF MEDICINE, ASSISTANT, 医歯学総合研究科, 講師 (40294409)
MATSUMOTO Yoshihito KAGAWA UNIVERSITY, SCHOOL OF MEDICINE, LECTURE, 医学部, 講師 (80311827)
KAWAI Nobuyuki KAGAWA UNIVERSITY, HOSPITAL, LECTURE, 医学部附属病院, 講師 (40294756)
SUGIU Kenji OKAYAMA UNIVERSITY, SCHOOL OF MEDICINE, ASSISTANT, 医学部附属病院, 講師 (40325105)
TOTUNAGA Koiji OKAYAMA UNIVERSITY, SCHOOL OF MEDICINE, ASSISTANT, 医学部附属病院, 講師 (40294467)
富田 享 岡山大学, 医学部附属病院, 講師 (90237115)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥10,500,000 (Direct Cost: ¥10,500,000)
Fiscal Year 2003: ¥4,700,000 (Direct Cost: ¥4,700,000)
Fiscal Year 2002: ¥5,800,000 (Direct Cost: ¥5,800,000)
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| Keywords | gene therapy / gene expression / glioma / Herpes virus vector / EB virus vector / Adenovirus vector / Cytosin deaminase gene / transferrin receptor / MRI |
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| Research Abstract |
Previously, we evaluated the therapeutic efficacy of the virus-mediated transduction of the chemosensitivity genes and prodrugs for malignant gliomas such as 5-fluorocytosine (5-FC)/cytosine deaminase (CD) gene therapy.
Firstly, we investigated the ability of radiation therapy to enhance 5-fluorocytosine (5-FC)/cytosine deaminase (CD) plus uracil phosphoribosyltransferase (UPRT) gene therapy in malignant gliomas. This combination therapy may be feasible for the treatment of gliomas, although the radiation dose and area should be reduced in order to prevent side effects (Cancer Gene Therapy 9 : 840-845, 2002).
Secondly, we examined the induction of apoptosis and the role of caspases in 5-FC/CD gene therapy using human malignant glioma cells. These results indicate that 5-FC/CD gene therapy induces apoptosis in human malignant glioma cells and that the apoptotic cell death is mediated by the activation of mitochondrial caspase cascades involving caspases 3 and 9 (J of Neuro-Oncology 66 : 117-127, 2004).
Recently, we are making new HSV/EB hybrid-based amplicon vector system for transgene delivery and developing the use MRI for in vivo imaging of transgene expression employing engineered transferrin receptor.
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