Electrophysiological study of the mechanism of unconsciousness of anesthesia
| Project/Area Number |
14370485
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | Shiga University of Medical Science |
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Principal Investigator |
TAKENOSHITA Makoto Shiga University of Medical Science, Dept. of Anesthesiology, associate professor, 医学部, 助教授 (00144486)
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| Co-Investigator(Kenkyū-buntansha) |
SETO Tomoyoshi Shiga University of Medical Science, 医学部, 講師 (10335177)
SAI Yoshikazu Shiga University of Medical Science, 医学部, 助教授 (50135703)
NOSAKA Shuichi Shiga University of Medical Science, 医学部, 教授 (80237833)
牧浦 弥恵子 滋賀医科大学, 医学部, 助手 (90293836)
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| Project Period (FY) |
2002 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥14,000,000 (Direct Cost: ¥14,000,000)
Fiscal Year 2004: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2003: ¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 2002: ¥8,800,000 (Direct Cost: ¥8,800,000)
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| Keywords | anesthesia / consciousness / thalamus / inhibitory synapse |
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| Research Abstract |
The effect of antianesthetic drug on the GABAergic current induced by anesthetics were investigated.
Method : Rats (17-20 days old) were used. Whole brain was removed and sliced in the cold icy saline. Thalalmic LD cells were identified under microscope. Whole cell recordings were made and GABAergic inhibitory currents were recorded while perfused with Ian solution bubbled with 95% O2 and 5% CO2. The effect of anesthetics (0.5% halothane and 0.5% sevoflurane) was first recorded, and then myristate or doxapram was applied with anesthetics for 10 to 15 min. and washed out.
Result : Anesthetic-induced augmentation of GABAergic currents was slightly subsided by myristate or doxapram.
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Report
(4 results)
Research Products
(5 results)
