| Project/Area Number |
14370509
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Nagoya University |
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Principal Investigator |
ONO Yoshinari (2004) Nagoya University, Graduate School of Medicine, Associate Professor, 大学院・医学系研究科, 助教授 (00301218)
大島 伸一 (2002-2003) 名古屋大学, 大学院・医学系研究科, 教授 (80293702)
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| Co-Investigator(Kenkyū-buntansha) |
HATTORI Ryohei Nagoya University, University Hospital, Assistant Professor, 医学部附属病院, 講師 (20324410)
YAMAMOTO Tokunori Nagoya University, Graduate School of Medicine, Research Associate, 大学院・医学系研究科, 助手 (20182636)
YOSHINO Yasushi Nagoya University, University Hospital, Research Associate, 医学部附属病院, 助手 (70324415)
小野 佳成 名古屋大学, 大学院・医学系研究科, 助教授 (00301218)
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| Project Period (FY) |
2002 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥11,700,000 (Direct Cost: ¥11,700,000)
Fiscal Year 2004: ¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 2003: ¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2002: ¥6,200,000 (Direct Cost: ¥6,200,000)
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| Keywords | peritubular capillary / microcirculation / a magnifying-endoscopy enabling / cadaveric transplants / living donor transplants / warm and cold ischemia / acute tubular necrosis / direct imaging system / Human kidney transplantation / 慢性移植腎腎症 / chronic rejection, hyperfiltration / renal microcirculation / ischemic damage / 腎阻血再灌流障害 / 腎移植 human kidney transplantation / chronic rejection / hyperfiltration / ischenmic damage / 腎移植Human kidney trasplantation / ishenic damege |
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| Research Abstract |
We developed a direct imaging system of renal microcirculation by a magnifying-endoscopy enabling visualization of the movement of erythrocyte in glumerular and cortical peritubular capillary(CPC). We investigated the microcirculation of CPC in the early phase of both living and cadaveric donor transplant kidneys. Erythrocyte velocity in CPC were monitored and measured in twenty renal transplants at 20,60,90 and 120 minutes after reperfusion. The kidney grafts came from 11 living donors and 9 non-heart beating cadaveric donors. In living donor transplants, erythrocyte velocity in CPC at 20 minutes after revascularization declined to one third of base line value just before nephrectomy, and recovered to the pre-nephrectomy value 120minutes after reperfusion. In contrast, it continued to be disturbed for 90 minutes in cadaveric donor transplants. Erythrocyte velocity in CPC more significantly deteriorated in cadaveric transplants than in those of living transplants at 20 through 60 minutes after the revascularization.
In living donor transplants, erythrocyte velocity did not correlate with donor age, both warm and cold ischemic time, time to the initial urination and best creatine clearance. In the cadaveric transplants ischemic time, both WIT and CIT, did not correlate with the erythrocyte velocity. However, donor age, duration of ATN and best creatine clearance after transplantation significantly correlated with the erythrocyte velocity.
The measurement of erythrocyte velocity in CPC is a reliable method for predicting the recovery of renal function and reserved renal function of kidney allografts undergoing prolonged ischemia.
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