| Project/Area Number |
14370583
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
TAKATA Takashi Hiroshima University, Graduate School of Biomedical Sciences, Professor, 大学院・医歯学総合研究科, 教授 (10154783)
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| Co-Investigator(Kenkyū-buntansha) |
MIYAUCHI Mutsumi Hiroshima University, Graduate School of Biomedical Sciences, Associate Professor, 大学院・医歯学総合研究科, 助教授 (50169265)
OGAWA Ikuko Hiroshima University, Hospital, Assistant Professor, 病院・講師 (70136092)
KUDO Yasusei Hiroshima University, Graduate School of Biomedical Sciences, Research Associate, 大学院・医歯学総合研究科, 助手 (50314753)
UCHIDA Takashi Hiroshima University, Graduate School of Biomedical Sciences, Professor, 大学院・医歯学総合研究科, 教授 (50150305)
MATSUDA Naoki Nagasaki University, Isotope Center, Professor, アイソトープセンター, 教授 (00304973)
佐藤 淳 広島大学, 大学院・医歯薬学総合研究科, 助手 (70335660)
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| Project Period (FY) |
2002 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥13,800,000 (Direct Cost: ¥13,800,000)
Fiscal Year 2004: ¥4,300,000 (Direct Cost: ¥4,300,000)
Fiscal Year 2003: ¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥5,900,000 (Direct Cost: ¥5,900,000)
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| Keywords | enamel proteins / ameloblastin / periodontal tissue / tissue regeneration / peptide / periodontal ligament / calcification / cementum / 歯周靭帯細胞 / 骨芽細胞 / シグナル伝達 |
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| Research Abstract |
To identify the bioactive components and sites in Emdogain (EMD), which is widely used as a periodontal regenerative therapy, and to explore the possibility of developing new medicament using the synthetic peptide of the active sites for periodontal tissue regeneration, we achieved the following studies. 1.Cellular and molecular mechanism of stimulative effects of EMD on proliferation and differentiation of periodontal cells such as periodontal ligament cells (PDLC), gingival epithelial cells, gingival fibroblasts and osteoblasts. 2.Signal pathways and receptors of EMD, 3.Determination of active components and sites of EMD, and 4.Effects of the synthetic peptide of the active site on PDLC proliferation and differentiation in vitro and periodontal tissue regeneration in vivo. The results of the studies were 1.EMD stimulated proliferation and differentiation of PDLC and osteoblasts and inhibited those of gingival epithelial cells and fibroblasts, 2.RTK-ERK 1/2 pathway was possibly involved in mitogenic response of PDLC to EMD, 3.An anti-ameloblastin antibody suppressed stimulatory effects of EMD on PDLC proliferation and differentiation, and 4.The synthetic peptide of the active site of ameloblastin stimulated proliferation and differentiation of PDLC and periodontal tissue regeneration. In conclusions, EMD has suitable bioactivities to periodontal tissue regeneration and the synthetic peptide determined in the present study could be used as a new periodontal tissue regeneration therapy.
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