A defense mechanism of a living body in the odontoblastic complex via gap junction
| Project/Area Number |
14370614
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Conservative dentistry
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
IKEDA Hideharu Tokyo Medical and Dental University, Dental Hospital, Lecturer, 歯学部附属病院, 講師 (20222896)
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| Co-Investigator(Kenkyū-buntansha) |
SUDA Hideaki Tokyo Medical and Dental University, Graduate School, Professor, 大学院・医歯学総合研究科, 教授 (00114760)
砂川 光宏 東京医科歯科大学, 歯学部附属病院, 助教授 (30179288)
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| Project Period (FY) |
2002 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥8,600,000 (Direct Cost: ¥8,600,000)
Fiscal Year 2004: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2003: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2002: ¥5,200,000 (Direct Cost: ¥5,200,000)
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| Keywords | odontoblast / gap junction / dye coupling / electrical coupling / tooth pulp / dentinal tubules / dentinal fluid flow / tooth pain / 象牙芽細胞 / Gap junction / イオンチャンネル / 象牙細管 / transjunctional conductance / 生体防御 / 象牙細管内容液の流れ / 象牙質細胞 / gap-junction / 歯髄 / 細芽細管 / patch-clamp法 / dual patch / dye-coupling |
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| Research Abstract |
In our series of studies, we electrophysiologically obtained the function of odontoblasts and a direct, non-neural transduction mechanism between odontoblasts and between odontoblasts and neighboring cells. We recognized dentinal tubules as that have a close relation with odontoblasts as the pathway for several stimuli, and focused on the defense mechanism of outward flow of dentinal fluid, microcirculation of dental pulp and chronic dental pain.
We revealed
1.Dye coupling using low molecule dye
2.Rack of evidence that there were voltage dependent currents in feline odontoblasts
3.Defense mechanism of the outward flow against the LPS entry through dentinal tubules of intact and caries affected dentin
4.Evidence of a functional syncytium of odontoblast complex
5.Effect of aging on electrical conductance between odontoblasts
6.Effect of extracellular pH on electrical conductance between odontoblasts
7.Effect of mechanical distortion of odontoblast cell membrane electrical conductance between odontoblast
8.Application of whole-cell voltage-clamp method to feline odontoblast
9.Gap junction between human odontoblasts and neighboring cells
10.Effect of iontophoresis and pulpward hydrostatic pressure on drug delivery through dentinal tubules 1
11.Effect of pulpal components on dentin permeability
12.Changes in human sensation and neural activity with aging
13.Neuropathic orofacial pain evoked by cold stimulus of oral mucosa
14.Microcirculation in the dental pulp
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Report
(4 results)
Research Products
(48 results)
