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Effect of physical factors on osseointegration

Research Project

Project/Area Number 14370637
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 補綴理工系歯学
Research InstitutionThe University of Tokushima

Principal Investigator

ICHIKAWA Tetsuo  The University of Tokushima, Institute of Health Bio-Sciences, Professor, 大学院・ヘルスバイオサイエンス研究部, 教授 (90193432)

Co-Investigator(Kenkyū-buntansha) TOMOTAKE Yoritoki  The University of Tokushima, Institute of Health Bio-Sciences, Research Associate, 大学院・ヘルスバイオサイエンス研究部, 助手 (70263853)
KAWAMOTO Naeko  The University of Tokushima, Medical & Dental Hospital, Research Associate, 医学部・歯学部附属病院, 助手 (40335823)
WATANABE Megumi  The University of Tokushima, Medical & Dental Hospital, Research Associate, 医学部・歯学部附属病院, 助手 (40380050)
山下 菊治  徳島大学, 歯学部, 助教授 (30182497)
蟹谷 英生  徳島大学, 歯学部, 助手 (10294711)
Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥7,800,000 (Direct Cost: ¥7,800,000)
Fiscal Year 2004: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2003: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 2002: ¥4,100,000 (Direct Cost: ¥4,100,000)
Keywordsmechanical stress / compressing force / magnetic field / micro pulse magnetic field / Cox2 / cDNA microarray / Hsp25 / immune reaction / 免疫応答 / 骨芽細胞 / 細胞外マトリクス / 骨形成 / Osseointegration / 物理的因子 / 磁場 / in vitro / in vivo / 組織 / リモデリング
Research Abstract

It is well known that appropriate occlusal force promotes bone formation around implants, whereas bone atorophy and bone resorption develop under no mechanical stress or overstressing. It has been reported that when shear stress loads to osteoblasts, increase of intracellular Ca^<2+> concentration, activation of protein kinase A, and upregulation of cyclooxygenase 2 (Cox-2) gene result in differentiation of osteoblasts. The purpose of this study was to clarify appropriate levels of stress for the bone remodeling and mechanotransduction signaling at molecular levels.
We examined pattern of gene expression when compressing force was directly loaded to a mouse osteoblast like cell line (MC3T3-E1) for 24 hours by reverse transcription polymerase chain reaction and cDNA macroarray. Furthermore, we examined change of gene expression under its stress by cDNA microarray.
Cox-2, c-fos, and alkaline phosphatase mRNAs were upregulated in proportion to the stress value when compressing force was loaded. The analysis of cDNA macroarray showed that expression of bone morphogenetic protein 4 (BMP4) and BMP7, which promote differentiation of osteoblasts, was upregulated. No differences of survival of MC3T3-E1 cells were observed between loading and unloading of stress. Furthermore, the analysis of cDNA microarray showed that compressing force upregulated expression of heat shock protein 25kDa (Hsp25). And when MC3T3-E1 cells were cultured in magnetic field and micro pulse magnetic field, Cox-2, c-fos, and alkaline phosphatase mRNAs were upregulated.
The present study indicates that the compression force promotes differentiation of MC3T3-E1 cells through the signaling pathway upregulating Hsp25.
In the study of immune reaction of dendritic cells to titaniumtitanium could not induce the immune reactions easily. It may be caused by the interface of Ti and immune cells which are ‘static' environment.

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (13 results)

All 2004 2003 2002

All Journal Article (11 results) Book (2 results)

  • [Journal Article] インプラント義歯の適合度 第1章 インプラントの臨床・技工の考え方と基礎知識 4 インプラント技工の変遷と課題 3)インプラント技工の適合度2004

    • Author(s)
      斎賀明彦, 石田 修, 市川哲雄
    • Journal Title

      歯科技工別冊 2004インプラントの技工

      Pages: 30-31

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 光造形法 第3章 症例から見るインプラント技工の実際 4 即時インプラント補綴法 1)光造形法2004

    • Author(s)
      友竹偉則, 市川哲雄
    • Journal Title

      歯科技工別冊 2004インプラントの技工

      Pages: 128-130

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] In vitro Study of Stress Distribution on Additional Posterior Mandibular Implants2004

    • Author(s)
      Wei Hua, Tomotake Yoritoki, Nagao Kan, Oguri Takafumi, Nagao Daisuke, Ichikawa Tetsuo
    • Journal Title

      Prosthodontic Research & Practice 3

      Pages: 84-91

    • NAID

      10013728469

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] New Magnetic Applications in Clinical Dentistry (Minoru Ai, Uuh-Yuan Shiau)2004

    • Author(s)
      Tetsuo Ichikawa, Naeko Kawamoto
    • Journal Title

      Biological Effects of Magnetic Attachment on the Human Body and Tissue (Quintessence books)

      Pages: 44-47

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] In vitro Study of Stress Distribution on Additional Posterior Mandibular Implants2004

    • Author(s)
      Wei Hua, Tomotake Yoritoki, Nagao Kan, Oguri Takafumi, Nagao Daisuke, Ichikawa Tetsuo
    • Journal Title

      Prosthodontic Research & Practice 3(1)

      Pages: 84-91

    • NAID

      10013728469

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] In vitro Study of Stress Distribution on Additional Posterior Mandibular Implants2004

    • Author(s)
      Wei Hua, Tomotake Yoritoki, Nagao Kan, Oguri Takafumi, Nagao Daisuke, Ichikawa Tetsuo
    • Journal Title

      Prosthodontic Research & Practice 3・1

      Pages: 84-91

    • NAID

      10013728469

    • Related Report
      2004 Annual Research Report
  • [Journal Article] スクリュー締め付け回転角/トルク計測によるインプラント上部構造の適合性評価2003

    • Author(s)
      齋賀明彦, 市川哲雄, 友竹偉則, 蟹谷英生, 本釜聖子
    • Journal Title

      日本補綴歯科学会雑誌 47

      Pages: 353-360

    • NAID

      10010529528

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 欠損補綴の選択肢インプラント適応条件をどのように考えるか2003

    • Author(s)
      大栗孝文, 友竹偉則, 市川哲雄
    • Journal Title

      別冊the Quintessence YEARBOOK 2003 現代の治療指針

      Pages: 336-337

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Fracture mechanisms of retrieved titanium screw thread in dental implant2002

    • Author(s)
      Ken'ichi Yokoyama, Tetsuo Ichikawa, Hiroki Murakami, Youji Miyamoto, Kenzo Asaoka
    • Journal Title

      Biomaterials 23

      Pages: 2459-2465

    • NAID

      10012357492

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Dynamics of bone marrow pressure with tapping of titanium and hydroxyapatite implants in rabbits2002

    • Author(s)
      Yoritoki Tomotake, Tetsuo Ichikawa, Hideo Kanitani, Masahide Nakao, Naoki Kitaoka
    • Journal Title

      Clinical Oral Implants Research 13(2)

      Pages: 221-224

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Immediate implant-supported oral rehabilitation using a photocurable plastic skull model and laser welding : A technical Note of Screw Retained type : Part I2002

    • Author(s)
      Tomotake Y, Kanitani H, Ichikawa T
    • Journal Title

      International Journal of Prosthodontics 15(3)

      Pages: 303-306

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] New Magnetic Application in Clinical Dentistry (Minoru Ai, Uuh-Yuan Shiau) Biological Effects of Magnetic Attachment on the Human Body and Tissue (44-47)2004

    • Author(s)
      Tetuo Ichikawa, Naeko Kawamoto
    • Publisher
      Quintessence books
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] New Magnetic Applications in Clinical Dentistry(Minoru Ai, Uuh-Yuan Shiau) Biological Effects of Magnetic Attachment on the Human Body and Tissue(44-47)2004

    • Author(s)
      Tetsuo Ichikawa, Naeko Kawamoto
    • Publisher
      Quintessence books
    • Related Report
      2004 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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