| Project/Area Number |
14370734
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | MUKOGAWA WOMEN'S UNIVERSITY |
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Principal Investigator |
HAGINAKA Jun Mukogawa Women's University, Pharmaceutical Sciences, Professor, 薬学部, 教授 (20164759)
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| Co-Investigator(Kenkyū-buntansha) |
HOSOYA Ken Kyoto Institute of Technology, Polymer Science and Engineering, Associate Professor, 繊維学部, 助教授 (00209248)
三瓶 春代 武庫川女子大学, 薬学部, 助手 (00312132)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥14,200,000 (Direct Cost: ¥14,200,000)
Fiscal Year 2003: ¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 2002: ¥11,500,000 (Direct Cost: ¥11,500,000)
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| Keywords | molecularly imprinted polymer / biomacromolecule / reversed micelle technique |
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| Research Abstract |
The aim of this study is to develop the novel preparation method of uniformly sized, molecularly imprinted polymers(MIPs) and to apply the method for the preparation of MIPs for a biomacromolecule such as a protein or nucleic acid. We tried to develop a novel preparation method using a glass or silica membrane with a relatively uniform, pore. First, a Shirasu porous glass(SPG) or porous silica membrane was reacted with octadecyltrimethylchlorosilane. Then a mixture of glyceroldimethacrylate, acrylamide, methanol and water was emulsified through hydrophobilized SPG or silica membranes in the presence of oxytocin as a template molecule. The emulsion of monomers obtained was then thermally polymerized. The obtained MIPs prepared using porous silica membranes gave more uniformly sized particles than those using SPG membranes. With regard to molecular recognition properties, the MIPs prepared by both methods gave molecular recognition for oxytocin, compared with non-imprinted polymers. Further studies are required to apply the proposed method for the preparation of MIPs for a biomacromolecule such as a protein or nucleic acid.
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