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Accelerated degradation of tumor suppressor gene products in human cancers

Research Project

Project/Area Number 14380304
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional biochemistry
Research InstitutionHAMAMATSU UNIVERSITY SCHOOL OF MEDICINE

Principal Investigator

KITAGAWA Masatoshi  HAMAMATSU UNIVERSITY, Medicine, Professor, 医学部, 教授 (50294971)

Co-Investigator(Kenkyū-buntansha) KITAGAWA Kyoko  HAMAMATSU UNIVERSITY, Medicine, Assistant Professor, 医学部, 助手 (20299605)
UCHIDA Chiharu  HAMAMATSU UNIVERSITY, Medicine, Assistant Professor, 医学部, 助手 (60223567)
ODA Toshiaki  HAMAMATSU UNIVERSITY, Medicine, Associate Professor, 医学部, 助教授 (90126805)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥15,100,000 (Direct Cost: ¥15,100,000)
Fiscal Year 2003: ¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 2002: ¥10,100,000 (Direct Cost: ¥10,100,000)
KeywordsUbiquitin ligase / p27^<Kip1> / RB protein / Cell cycle / cancer / prognosis / 癌
Research Abstract

Ubiquitin-proteasome pathway is a key system that controls cellular levels of number of proteins. This pathway is responsible not only for the degradation of short-lived proteins but also tumor suppressors, transcription factors and cell cycle proteins. Ubiquitin-protein ligases determine the substrate specificity of ubiquitination. In this study, we analyzed the molecular mechanisms of proteolytic degradation of two major tumor suppressor gene products such as p27^<Kip1> and RB protein. Fast of all, we tried to identify the ubiquitin ligase for RB protein and analyze its function in malignant transformation process (1). The other project is identification of the down-regulation mechanisms of p27^<Kip1> (2).
In this study, we demonstrated that Mdm2 promotes ubiqutination of RB protein as an ubiquitin ligase and proteasome-mediated degradation of it. Knockdown of Mdm2 significantly accumulated steady state levels of RB protein. In human lung cancer tissues, high expression of Mdm2 correlated with down regulation of RB protein. These results suggest that facilitation of degradation of RB protein by Mdm2 perturbs the tumor suppressor RB-pathway. On the other hand, we found that Cks1, a subcomponent of SCF-Skp2 ubiquitin ligase for p27-degradation, was post-translalionally regulated by ubiquitin-proteasome pathway. We also found high expression of Cks1 in human lung cancers. Therefore, altered degradation of these cell cycle proteins or tumor suppressors is thought to promote growth and malignancy of cancer.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (58 results)

All Other

All Publications (58 results)

  • [Publications] Uchida, C. et al.: "The role of Sp1 and AP-2 in basal and protein kinase A-induced expression of mitochondrial serine : pyruvate aminotransferase in hepatocytes."J.Biol.Chem.. 277. 39082-39092 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mizuno, T. et al.: "Analyses in transfected cells and in vitro of a putative peroxisomal targeting signal of rat liver serine : pyruvate aminotransferase."Histochem. Cell Biol.. 118. 321-328 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Isse, T. et al.: "Diminished alcohol preference in transgenic mice lacking aldehyde dehydrogenase activity."Pharmacogenetics. 12. 621-626 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tsukino, H. et al.: "Genetic polymorphisms of CYP2A6 and CYP2E1 with tabacco smoking is not associated with risk of urothelial cancer."Environ. Health Prev.Med.. 7. 129-131 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hattori, T. et al.: "Cks1 is degraded via the ubiquitin-proteasome pathway in a cell cycle-dependent manner."Genes to Cells. 8. 889-896 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Togawa, A. et al.: "Ubiquitin-dependent degradation of Smad2 is increased in the glomeruli of rats with anti-thymocyte serum nephritis."Am.J.Pathol.. 163. 1645-1652 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Natsume, H. et al.: "β-Catenin/Tcf-1-mediated transactivation of cyclin D1 promoter is negatively regulated by thyroid hormone."Biochem.Biophys.Res.Commun.. 309. 408-413 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Imaki, H. et al.: "Cell Cycle-dependent Regulation of the Skp2 Promoter by GA-binding Protein."Cancer Res.. 63. 4607-4613 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hayakawa, M. et al.: "Evidence that reactive oxygen species do not mediate NF-κB activation."EMBO J.. 22. 3356-3366 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Inui, N. et al.: "High expression of Cks1 in human non-small cell lung carcinomas."Biochem.Biophys.Res.Commun.. 303. 978-984 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tahara, K. et al.: "ΔFosB but not FosB induces delayed apoptosis independent of cell proliferation in the Rat1a embryo cell line."Cell Death and Diff.. 10. 496-507 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Katayama, K. et al.: "Cdk4/cyclin D1 kinase, a universal and critical regulator of apoptosis."Anticancer Res.. 23. 235-244 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Matsumoto, A. et al.: "Bisphenol A levels in human urine."Environmental Health Perspectives. 111. 101-104 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Huang, Y. et al.: "Altered antigenic disposition of peroxisomal urate oxidase in PEX5-defective Chinese hamster ovary cells."Biochem.Biophys.Res.Com.. 302. 703-709 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Inoue, Y. et al.: "Contribution of the constitutive and inducible degradation of Smad3 by the ubiquitin-proteasome pathway to transforming growth factor-β signaling."J. Interferon & Cytokine Research. 24. 43-54 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kimura, M. et al.: "Cell cycle-dependent regulation of the human aurora B promoter."Biochem Biophys Res Commun.. 316. 930-936 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fukasawa, H. et al.: "Treatment with anti-TGF-β antibody ameliorates progressive nephritis by inhibiting Smad/TGF-β signaling."Kidney Int.. 65. 63-74 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Matsumoto, M. et al.: "Molecular clearance of ataxin-3 is regulated by a mammalian E4."EMBO J.. 23. 659-669 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kawamoto, T. et al.: "Effects of genetic polymorphism of drug metabolizing enzymes on smoking and drinking."J. UOEH. 25. 97-106 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Oyama, T. et al.: "Expression of cytochrome P450 in tumor tissues and its association with cancer development."Frontier in Bioscience. 9. 1967-1976 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 北川雅敏(分担執筆): "細胞周期メカニズムの解明に向けて ポストゲノムの分子生物学 村上康文 編"化学同人. 95-106 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 北川雅敏: "現代医療36"現代医療社. 147-153 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Uchida, C. et al.: "The role of Sp1 and AP-2 in basal and protein kinase A-induced expression of mitochondrial serine : pyruvate aminotransferase in hepatocytes."J.Biol.Chem.. 277. 39082-39092 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mizuno, T. et al.: "Analyses in ttansfected cells and in vitro of a putative peroxisomal targeting signal of rat liver serine : pyruvate aminotransferase."Histochem. Cell Biol.. 118. 321-328 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Isse, T. et al.: "Diminished alcohol preference in transgenic mice lacking aldehyde dehydrogenase activity."Phamacogenetics. 12. 621-626 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tsukino, H. et al.: "Genetic polymorphisms of CYP2A6 and CYP2E1 with tabacco smoking is not associated with risk of urothelial cancer."Environ. Health Prey. Med.. 7. 129-131 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hattori, T. et al.: "Cks1 is degraded via the ubiquitin-proteasome pathway in a cell cycle-dependent manner."Genes to Cells. 8. 889-896 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Togawa, A. et al.: "Ubiquitin-dependent degradation of Smad2 is increased in the glomeruli of rats with anti-thymocyte serum nephritis."Am.J.Pathol.. 163. 1645-1652 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Natsume, H. et al.: "β-Catenin/Tcf-1-mediated transactivation of cyclin D1 promoter is negatively regulated by thyroid hormone."Biochem.Biophys.Res.Commun.. 309. 408-413 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Imaki, H. et al.: "Cell Cycle-dependent Regulation of the Skp2 Promoter by GA-binding Protein."Cancer Res.. 63(15). 4607-4613 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hayakawa, M. et al.: "Evidence that reactive oxygen species do not mediate NF-κB activation."EMBO J.. 22. 3356-3366 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Inui, N. et al.: "High expression of Cks1 in human non-small cell lung carcinomas."Biochem.Biophys.Res.Commun. 303. 978-984 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tahara, K. et al.: "Δ FosB but not FosB induces delayed apoptosis independent of cell proliferation in the Rat1a embryo cell line."Cell Death and Diff. 10. 496-507 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Katayama, K. et al.: "Cdk4/cyclin D1 kinase, a universal and critical regulator of apoptosis."Anticancer Res.. 23. 235-244 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Matsumoto, A. et al.: "Bisphenol A levels in human urine."Environmental Health Perspectives. 111. 101-104 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Huang, Y. et al.: "Altered antigenic disposition of peroxisomal urate oxidase in PEX5-defective Chinese hamster ovary cells."Biochem.Biophys.Res.Com.. 302. 703-709 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Inoue, Y. et al.: "Contribution of the constitutive and inducible degradation of Smad3 by the ubiquitin-proteasome pathway to transforming growth factor-β signaling."J. Interferon & Cytokine Research. 24. 43-54 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kimura, M. et al.: "Cell cycle-dependent regulation of the human aurora B promoter."Biochem Biophys Res Commun.. 316(3). 930-936 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fukasawa, H. et al.: "Treatment with anti-TGF-β antibody ameliorates progressive nephritis by inhibiting Smad/TGF-β signaling."Kidney Int.. 65. 63-74 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Matsumoto, M. et al.: "Molecular, clearance of ataxin-3 is regulated by a mammalian E4."EMBO J. 23. 659-669 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kawamoto, T. et al.: "Effects of genetic polymorphism of drug metabolizing enzymes on smoking and drinking."J UOEH. 25. 97-106 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Oyama, T. et al.: "Expression of cytochrome P450 in tumor tissues and its association with cancer development."Frontier in Bioscience. 9. 1967-1976 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Katayama, K.et al.: "Cdk4/cyclin D1 kinase, a universal and critical regulator of apoptosis."Anticancer Res.. 23. 235-244 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Tahara, K.et al.: "ΔFosB but not FosB induces delayed apoptosis independent of cell proliferation in the Rat1a embryo cell line."Cell Death and Diff.. 10. 496-507 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Inui, N.et al.: "High expression of Cks1 in human non-small cell lung carcinomas."Biochem.Biophys.Res.Commun. 303. 978-984 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hayakawa, M.et al.: "Evidence that reactive oxygen species do not mediate NF-κB activation."EMBO J.. 22. 3356-3366 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Imaki, H.et al.: "Cell Cycle-dependent Regulation of the Skp2 Promoter by GA-binding Protein."Cancer Res.. 63. 4607-4613 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Natsume, H.et al.: "β-Catenin/Tcf-1-mediated transactivation of cyclin D1 promoter is negatively regulated by thyroid hormone."Biochem.Biophys.Res.Commun.. 309. 408-413 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Togawa, A.et al.: "Ubiquitin-dependent degradation of Smad2 is increased in the glomeruli of rats with anti-thymocyte serum nephritis."Am.J.Pathol.. 163. 1645-1652 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hattori, T.et al.: "Cks1 is degraded via the ubiquitin-proteasome pathway in a cell cycle-dependent manner."Genes to Cells. 8. 889-896 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Fukasawa, H.et al.: "Treatment with anti-TGF-β antibody ameliorates progressive nephritis by inhibiting Smad/TGF-β signaling."Kidney Int.. 65. 63-74 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 北川雅敏(分担執筆): "ポストゲノムの分子生物学「細胞周期メカニズムの解明に向けて」(村上康文 編)"化学同人社. 184 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Uchida, C. et al.: "The role of Sp1 and Ap-2 in basal and protein kinase A-induced expression of mitochondrial serine : pyruvate aminotransferase in hepatocytes"Journal of Biological Chemistry. 277. 39082-39092 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Mizuno, T., et al.: "Analyses in transfected cells and in vitro of a putative peroxisomal targeting signal of rat liver serine : pyruvate aminotransferase"Histochem. Cell Biol.. 118. 321-328 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Matsumoto A. et al.: "Bisphenol a levels in human urine"Environ. Health Perspect.. 111. 101-104 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Katayama, K. et al.: "Cdk4/Cyclin D1 kinase, a universal and critical regulator of apoptosis"Anticancer Res.. 23. 235-244 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Inui, N. et al.: "High expression of Cks1 in human non-small cell lung carcinomas"Biochem. Biophys. Research. Communication. 303. 973-984 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Isse, T. et al.: "Diminished alcohol preference in transgenic mice lacking aldehyde dehydrogenase activity"Pharmacogenetics. 12. 621-626 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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