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Where is the pump switch of bacteriorhodopsin located?

Research Project

Project/Area Number 14380316
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biophysics
Research InstitutionNagoya Institute of Technology

Principal Investigator

KANDORI Hideki  Nagoya Institute of Technology, Materials Science and Engineering, Professor, 工学研究科, 教授 (70202033)

Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥15,000,000 (Direct Cost: ¥15,000,000)
Fiscal Year 2004: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 2003: ¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2002: ¥9,300,000 (Direct Cost: ¥9,300,000)
Keywordsbacteriorhodopsin / FTIR / proton pump / hydrogen bond / internal water molecule / light sensor / vectorial transport / isotope effect / 能動輸送 / エントロピー減少 / 光異性化反応 / 水素結合ネットワーク / 内部結合水
Research Abstract

This research project aims at revealing the mechanism of proton pump in bacteriorhodopsin(BR). For this aim, we applied low-temperature polarized Fourier-transform infrared(FTIR) spectroscopy. Highly accurate FTIR spectroscopy of BR and related proteins provided the following results.
(1)A new model for the primary proton transfer reaction is proposed based on the detailed structural analysis of internal water molecules in BR by low-temperature FTIR spectroscopy. This method led to the so called "hydration switch model". According to the model, the proton transfer reaction is controlled by a hydration switch of a water molecule from Asp85 to Asp212.
(2)By use of isotope label and mutant proteins, we identified stretching vibrations of the protonated Schiff base and Arg82,both of which constitute hydrogen-bonding network in BR.
(3)Hydrogen-bonding network in halorhodopsin, a light-driven chloride ion pump, is studied. We found that a chloride ion is hydrated by internal water molecules differently from those in BR.
(4)Complex formation between pharaonis phoborhodopsin and its transducer protein was detected by FTIR spectroscopy.
(5)Protein structural changes of Neurospora rhodopsin and visual rhodopsin were studied. Unique hydrogen-bonding structures are revealed.

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (95 results)

All 2005 2004 2003 2002 Other

All Journal Article (64 results) Publications (31 results)

  • [Journal Article] Water structural changes in the activation process of the LOV2 domain of Adiantum phytochrome32005

    • Author(s)
      D.Nozaki et al.
    • Journal Title

      J.Mol.Struct. 735

      Pages: 259-265

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Reactive cysteine is protonated in the triplet excited state of the LOV2 domain in Adiantum phytochrome32005

    • Author(s)
      Y.Sato et al.
    • Journal Title

      J.Am.Chem.Soc. 127

      Pages: 1088-1089

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Structural changes of the complex between pharaonis phoborhodopsin and its cognate transducer upon formation of the M photointermediate2005

    • Author(s)
      Y.Furutani et al.
    • Journal Title

      Biochemistry 44(In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Strongly hydrogen-bonded water molecules in the Schiff base region of rhodopsins2005

    • Author(s)
      Y.Furutani et al.
    • Journal Title

      Photochem.Photobiol.Sci. (In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] FTIR Spectroscopy of the O Photointermediate in pharaonis Phoborhodopsin2004

    • Author(s)
      Y.Furutani et al.
    • Journal Title

      Biochemistry (発表予定)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] FTIR spectroscopy of the O photointermedate in pharaonis phoborhodopcin2004

    • Author(s)
      Y.Furutani et al.
    • Journal Title

      Biochemistry 43

      Pages: 5204-5212

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Role of Gln1029 in the photoactivation processes of the LOV2 domain in Adiantum phytochrome32004

    • Author(s)
      D.Nozaki et al.
    • Journal Title

      Biochemistry 43

      Pages: 8373-8379

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Internal water molecules of light-driven chloride pump proteins2004

    • Author(s)
      M.Shibata et al.
    • Journal Title

      Chem.Phys.Lett. 392

      Pages: 330-333

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Altered hydrogen bonding of Arg82 during the proton pump cycle of bacteriorhodopsin : A low-temperature polarized FTIR spectroscopic study.2004

    • Author(s)
      T.Tanimoto et al.
    • Journal Title

      Biochemistry 43

      Pages: 9439-9447

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] FTIR spectroscopy of the K photointermedate of neurospora rhodopsin : Structural changes of the retinal, protein, and water molecules after photoisomerization2004

    • Author(s)
      Y.Furutani et al.
    • Journal Title

      Biochemistry 43

      Pages: 9636-9646

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Role of hydrogen-bond network in energy storage of bacteriorhodopsin's light-driven proton pump revealed by ab initio normal mode analysis.2004

    • Author(s)
      S.Hayashi et al.
    • Journal Title

      J.Am.Chem.Soc. 126

      Pages: 10516-10517

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Hydration switch model for the proton transfer in the Schiff base region of bacteriorhodopsin2004

    • Author(s)
      H.Kandori
    • Journal Title

      Biochim.Biophys.Acta 1658

      Pages: 72-79

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Molecular mechanism of protein-protein interaction of pharaonis phoborhodopsin/transducer and photo-signal transfer reaction by complex2004

    • Author(s)
      Y.Sudo et al.
    • Journal Title

      Recent Res.Devel.Biophys. 3

      Pages: 1-16

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] バクテリオロドプシンにおける内部結合水の振動解析2004

    • Author(s)
      柴田幹大, 神取秀樹
    • Journal Title

      生物物理 44

      Pages: 113-117

    • NAID

      110001151703

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] FTIR Spectroscopy of the O Photointermediate in pharaonis Phoborhodopsin2004

    • Author(s)
      Y.Furutani, M.Iwamoto, K.Shimono, A.Wada, M.Ito, N.Kamo, H.Kandori
    • Journal Title

      Biochemistry 43

      Pages: 5204-5212

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Role of Gln 1029 in the Photoactivation Processes of the LOV2 Domain in Adiantum Phytochrome32004

    • Author(s)
      D.Nozaki, T.Iwata, T.Ishikawa, T.Todo, S.Tokutomi, H.Kandori
    • Journal Title

      Biochemistry 43

      Pages: 8373-8379

    • NAID

      130006986938

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Internal Water Molecules of Light-Driven Chloride Pump Proteins2004

    • Author(s)
      M.Shibata, N.Muneda, K.Ihara, T.Sasaki, M.Demura, H.Kandori
    • Journal Title

      Chem.Phys.Lett. 392

      Pages: 330-333

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Altered Hydrogen Bonding of Arg82 during the Proton Pump Cycle of Bacteriorhodopsin : A Low-Temperature Polarized FTIR Spectroscopic Study2004

    • Author(s)
      T.Tanimoto, M.Shibata, M.Belenky, J.Herzfeld, H.Kandori
    • Journal Title

      Biochemistry 43

      Pages: 9439-9447

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] FTIR Spectroscopy of the K Photointermediate of Neurospora Rhodopsin ; Structural Changes of the Retinal, Protein, and Water Molecules after Photoisomerization.2004

    • Author(s)
      Y.Furutani, A.G.Bezarra Jr, S.Waschuk, M.Sumii, L.S.Brown, H.Kandori
    • Journal Title

      Biochemistry 43

      Pages: 9636-9646

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Role of Hydrogen-Bond Network in Energy Strage of Bacteriorhodopsin's Light-Driven Proton Pump Revealed by ab Initio Normal-Mode Analysis2004

    • Author(s)
      S.Hayashi, E.Tajkhorshid, H.Kandori, K.Schulten
    • Journal Title

      J.Am.Chem.Soc. 126

      Pages: 10516-10517

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] FTIR spectroscopy of the O photointermedate in pharaonis phoborhodopsin2004

    • Author(s)
      Y Furutani et al.
    • Journal Title

      Biochemistry 43

      Pages: 5204-5212

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Altered hydrogen bonding of Arg82 during the proton pump cycle of bacteriorhodopsin : A low-temperature polarized FTIR spectroscopic study2004

    • Author(s)
      T.Tanimoto et al.
    • Journal Title

      Biochemistry 43

      Pages: 9439-9447

    • Related Report
      2004 Annual Research Report
  • [Journal Article] FTIR spectroscopy of the K photointermedate of neurospora rhodopsin: Structural Changes of the retinal, protein, and water molecules after photoisomerization2004

    • Author(s)
      Y.Furutani et al.
    • Journal Title

      Biochemistry 43

      Pages: 9636-9646

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Structural Changes of Water in the Schiff Base Region of Bacteriorhodopsin : Proposal of a Hydration Switch Model2003

    • Author(s)
      T.Tanimoto et al.
    • Journal Title

      Biochemistry 42

      Pages: 2300-2306

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Proton Transfer Reactions in the F86D and F86E Mutants of Pharaonis Phoborhodopsin (Sensory Rhodopsin II)2003

    • Author(s)
      M.Iwamoto et al.
    • Journal Title

      Biochemistry 42

      Pages: 2790-2796

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] FTIR Spectroscopy of the complex between pharaonis Phoborhodopsin and its Transducer Protein2003

    • Author(s)
      Y.Furutani et al.
    • Journal Title

      Biochemistry 42

      Pages: 4837-4842

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Vibrational Modes of the Protonated Schiff Base in pharaonis Phoborhodopsin2003

    • Author(s)
      K.Shimono et al.
    • Journal Title

      Biochemistry 42

      Pages: 7801-7806

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Light-induced structural changes in the LOV2 domain of Adiantum phytochrome3 studied by low-temperature FTIR and UV-visible spectroscopy2003

    • Author(s)
      T.Iwata et al.
    • Journal Title

      Biochemistry 42

      Pages: 8183-8191

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Structural Changes in Lumirhodopsin and Metarhodopsin I Studied by their Photoreactions at 77K2003

    • Author(s)
      Y.Furutani et al.
    • Journal Title

      Biochemistry 42

      Pages: 8494-8500

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Structural Changes of Water Molecules during the Photoactivation Processes in Bovine Rhodopsin2003

    • Author(s)
      Y.Furutani et al.
    • Journal Title

      Biochemistry 42

      Pages: 9619-9625

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Water molecules in the Schiff Base Region of Bacteriorhodopsin2003

    • Author(s)
      M.Shibata et al.
    • Journal Title

      J.Am.Chem.Soc. 125

      Pages: 13312-13313

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Hydrogen bonding alteration of Thr204 in the complex between pharaonis phoborhodopsin and its transducer protein2003

    • Author(s)
      Y.Sudo et al.
    • Journal Title

      Biochemistry 42

      Pages: 14166-14172

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Photochemical properties of pharaonis phoborhodopsin (sensory rhodopsin II)2003

    • Author(s)
      M.Iwamoto et al.
    • Journal Title

      Recent Res.Develp.Chem. 1

      Pages: 15-30

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 視覚の初期過程における光化学反応2003

    • Author(s)
      神取秀樹
    • Journal Title

      レーザー研究 31

      Pages: 184-189

    • NAID

      80015843943

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Structural Changes of Water in the Schiff Base Region of Bacteriorhodopsin : Proposal of a Hydration Switch Model2003

    • Author(s)
      T.Tanimoto, Y.Furutani, H.Kandori
    • Journal Title

      Biochemistry 42

      Pages: 2300-2306

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Proton Transfer Reaction in the F86D and F86E Mutants of pharaonis Phoborhodopsin (Sensory Rhodopsin II)2003

    • Author(s)
      M.Iwamoto, Y.Furutani, N.Kamo, H.Kandori
    • Journal Title

      Biochemistry 42

      Pages: 2790-2796

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] FTIR Spectroscopy of the Complex between pharaonis Phoborhodopsin and its Transducer Protein2003

    • Author(s)
      Y.Furutani, Y.Sudo, N.Kamo, H.Kandori
    • Journal Title

      Biochemistry 42

      Pages: 4837-4842

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Vibrational Models of the Protonated Schiff Base in pharaonis Phoborhodopsin2003

    • Author(s)
      K.Shimono, Y.Furutani, N.Kamo, H.Kandori
    • Journal Title

      Biochemistry 42

      Pages: 7801-7806

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Light-Induced Structural Changes in the LOV2 Domain of Adiantum Phytochrome3 Studied by Low-Temperature FTIR and UV-Visible Spectroscopy2003

    • Author(s)
      T.Iwata, D.Nozaki, S.Tokutomi, T.Kagawa, M.Wada, H.Kandori
    • Journal Title

      Biochemistry 42

      Pages: 8183-8191

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Structural Changes in Lumirhodopsin and Metarhodopsin I Studied by Their Photoreactions at 77 K2003

    • Author(s)
      Y.Furutani, H.Kandori, Y.Shichida
    • Journal Title

      Biochemistry 42

      Pages: 8494-9500

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Structural Changes of Water Molecules during the Photoactivation Process in Bovine Rhodopsin2003

    • Author(s)
      Y.Furutani, Y.Shichida, H.Kandori
    • Journal Title

      Biochemistry 42

      Pages: 9619-9625

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Water Molecules in the Schiff Base Region of Bacteriorhodopsin2003

    • Author(s)
      M.Shibata, T.Tanimoto, H.Kandori
    • Journal Title

      J.Am.Chem.Soc. 125

      Pages: 13312-13313

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Hydrogen Bonding Alteration of Thr-204 in the Complex between pharaonis Phoborhodopsin aud Its Transducer Protein2003

    • Author(s)
      Y.Sudo, Y.Furutani, K.Shimono, N.Kamo, H.Kandori
    • Journal Title

      Biochemistry 42

      Pages: 14166-14172

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Isomer-Specific Interaction of the Retinal Chromophore with Threonine-118 in Rhodopsin2002

    • Author(s)
      T.Nagata et al.
    • Journal Title

      J.Phys.Chem.A 106

      Pages: 1969-1975

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Excited-State Dynamics of pharaonis Phoborhodopsin Probed by Femtosecond Fluorescence Spectroscopy2002

    • Author(s)
      H.Kandori et al.
    • Journal Title

      J.Phys.Chem.A 106

      Pages: 2091-2095

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Photoisomerization of the Rhodopsin Chromophore in Clay Interlayers at 77K2002

    • Author(s)
      H.Kandori et al.
    • Journal Title

      Chem.Phys.Lett. 354

      Pages: 251-255

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Interaction of Asn105 with the Retinal Chromophore during Photoisomerization of pharaonis Phoborhodopsin2002

    • Author(s)
      H.Kandori et al.
    • Journal Title

      Biochemistry 41

      Pages: 4554-4559

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Vibrational Frequency and Dipolar Orientation of the Protonated Schiff Base in Bacteriorhodopsin before and after Photoisomerization2002

    • Author(s)
      H.Kandori et al.
    • Journal Title

      Biochemistry 41

      Pages: 6026-6031

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A pharaonis Phoborhodopsin Mutant with the Same Retinal Binding Site Residues as in Bacteriorhodopsin2002

    • Author(s)
      K.Shimono et al.
    • Journal Title

      Biochemistry 41

      Pages: 6504-6509

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Role of Asp193 in Chromophore-Protein Interaction of pharaonis Phoborhodopsin (Sensory Rhodopsin II)2002

    • Author(s)
      M.Iwamoto et al.
    • Journal Title

      Biophys.J. 83

      Pages: 1130-1135

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Photoreaction of the Cysteine S-H Group in the LOV2 Domain of Adiantum Phytochrome32002

    • Author(s)
      T.Iwata et al.
    • Journal Title

      J.Am.Chem.Soc. 124

      Pages: 11840-11841

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] FTIR Spectroscopy of the M Photointermediate in pharaonis Phoborhodopsin2002

    • Author(s)
      Y.Furutani et al.
    • Journal Title

      Biophys.J. 83

      Pages: 3482-3489

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Internal Water Molecules of Archaeal Rhodopsins2002

    • Author(s)
      Y.Furutani, H.Kandori
    • Journal Title

      Mol.Membr.Biol. 19

      Pages: 257-265

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] バクテリオロドプシンのプロトンポンプ機構2002

    • Author(s)
      神取秀樹
    • Journal Title

      酵素工学ニュース 47

      Pages: 17-22

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] フォボロドプシンの結晶構造と波長制御・光情報伝達機構2002

    • Author(s)
      神取秀樹, 加茂直樹
    • Journal Title

      蛋白質核酸酵素 47

      Pages: 620-625

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Isomer-Specific Interaction of the Retinal Chromophore with Threonine-118 in Rhodopsin2002

    • Author(s)
      T.Nagata, T.Oura, A.Terakita, H.Kandori, Y.Shichida
    • Journal Title

      J.Phys.Chem.A 106

      Pages: 1969-1975

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Excited-State Dynamics of pharaonis Phoborhodopsin Probed by Femtosecond Fluorescence Spectroscopy2002

    • Author(s)
      H.Kandori, H.Tomioka, H.Sasabe
    • Journal Title

      J.Phys.Chem.A 106

      Pages: 2091-2095

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Photoisomerization of the Rhodopsin Chromophore in Clay Interlayers at 77 K2002

    • Author(s)
      H.Kandori, T.Ichioka, M.Sasaki
    • Journal Title

      Chem.Phys.Lett. 354

      Pages: 251-255

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Interaction of Asn105 with the Retinal Chromophore during Photoisomerization of pharaonis Phoborhodopsin2002

    • Author(s)
      H.Kandori, K.Shimono, Y.Shichida, N.Kamo
    • Journal Title

      Biochemistry 41

      Pages: 4554-4559

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Vibrational Frequency and Dipolar Orientation of the Protonated Schiff Base in Bacteriorhodopsin before and after Photoisomerization.2002

    • Author(s)
      H.Kandori, M.Belenky, J.Herzfeld
    • Journal Title

      Biochemistry 41

      Pages: 6026-6031

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A pharaonis Phoborhodopsin Mutant with the Same Retinal Binding Site Residues as in Bacteriorhodopsin2002

    • Author(s)
      K.Shimono, Y.Furutani, H.Kandori, N.Kamo
    • Journal Title

      Biochemistry 41

      Pages: 6504-6509

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Role of Asp193 in Chromophore-Protein Interaction of pharaonis Phoborhodopsin (Sensory Rhodopsin II)2002

    • Author(s)
      M.Iwamoto, Y.Furutani, Y.Sudo, K.Shimono, H.Kandori, N.Kamo
    • Journal Title

      Biophys.J. 83

      Pages: 1130-1135

    • NAID

      120000963626

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Photoreaction of the Cysteine S-H Group in the LOV2 Domain of Adiantum Phytochrome32002

    • Author(s)
      T.Iwata, S.Tokutomi, H.Kandori
    • Journal Title

      J.Am.Chem.Soc. 124

      Pages: 11840-11841

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] FTIR Spectroscopy of the M Photointermediate in pharaonis Phoborhodopsin2002

    • Author(s)
      Y.Furutani, M.Iwamoto, K.Shimono, N.Kamo, H.Kandori
    • Journal Title

      Biophys.J. 83

      Pages: 3482-3489

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] T.Tanimoto et al.: "Structural Changes of Water in the Schiff Base Region of Bacteriorhodopsin : Proposal of a Hydration Switch Model"Biochemistry. 42. 2300-2306 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] M.Iwamoto et al.: "Proton Transfer Reactions in the F86D and F86E Mutants of Pharaonis Phoborhodopsin (Sensory Rhodopsin II)"Biochemistry. 42. 2790-2796 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Y.Furutani et al.: "FTIR Spectroscopy of the complex between pharaonis Phoborhodopsin and its Transducer Protein"Biochemistry. 42. 4837-4842 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] K.Shimono et al.: "Vibrational Modes of the Protonated Schiff Base in pharaonis Phoborhodopsin"Biochemistry. 42. 7801-7806 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] T.Iwata et al.: "Light-induced structural changes in the LOV2 domain of Adiantum phytochrome3 studied by low-temperature FTIR and UV-visible spectroscopy"Biochemistry. 42. 8183-8191 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Y.Furutani et al.: "Structural Changes in Lumirhodopsin and Metarhodopsin I Studied by their Photoreactions at 77 K"Biochemistry. 42. 8494-8500 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Y.Furutani et al.: "Structural Changes of Water Molecules during the Photoactivation Processes in Bovine Rhodopsin"Biochemistry. 42. 9619-9625 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] M.Shibata et al.: "Water molecules in the Schiff Base Region of Bacteriorhodopsin"J.Am.Chem Soc.. 125. 13312-13313 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Y.Sudo et al.: "Hydrogen bonding alteration of Thr2O4 in the complex between pharaonis phoborhodopsin and its transducer protein"Biochemistry. 42. 14166-14172 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Y.Furutani et al.: "FTIR Spectroscopy of the O Photointermediate in phdraonis Phoborhodopsin"Biochemistry. (発表予定). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] M.Iwamoto et al.: "Photochemical properties of pharaonis phoborhodopsin (sensory rhodopsin II)"Recent Res.Develp.Chem.. 1. 15-30 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 神取秀樹: "視覚の初期過程における光化学反応"レーザー研究. 31. 184-189 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 神取秀樹: "ロドプシンを究める"日本化学会、生体機能関連化学部会News Letter. 17. 10-13 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] H.Kandori: "Hydration Switch Model for the proton transfer in the Schiff base region of bacteniorhodopsin"Biochim.Biophys.Acta. (In press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 柴田幹大, 神取秀樹: "バクテリオロドプシンにおける内部結合水の振動解析"生物物理. (印刷中). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] T.Nagata et al.: "Isomer-Specific Interaction of the Retinal Chromophore with Threonine-118 in Rhodopsin"J.Phys.Chem.A. 106. 1969-1975 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] H.Kandori et al.: "Excited-State Dynamics of pharaonis Phoborhodopsin Probed by Femtosecond Fluorescence Spectroscopy"J.Phys.Chem.A. 106. 2091-2095 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] H.Kandori et al.: "Photoisomerization of the Rhodopsin Chromophore in Clay Interlayers at 77K"Chem.Phys.Lett.. 354. 251-255 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] H.Kandori et al.: "Interaction of Asn105 with the Retinal Chromophore during Photoisomerization of pharaonis Phoborhodopsin"Biochemistry. 41. 4554-4559 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] H.Kandori et al.: "Vibrational Frequency and Dipolar Orientation of the Protonated Schiff Base in Bacteriorhodopsin before and after Photoisomerization"Biochemistry. 41. 6026-6031 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] K.Shimono et al.: "A pharaonis Phoborhodopsin Mutant with the Same Retinal Binding Site Residues as in Bacteriorhodopsin"Biochemistry. 41. 6504-6509 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] M.Iwamoto et al.: "Role of Asp193 in Chromophore-Protein Interaction of pharaonis Phoborhodopsin (Sensory Rhodopsin II)"Biophys.J.. 83. 1130-1135 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] T.Iwata et al.: "Photoreaction of the Cysteine S-H Group in the LOV2 Domain of Adiantum Phytochrome3"J.Am.Chem Soc.. 124. 11840-11841 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Y.Furutani et al.: "FTIR Spectroscopy of the M Photointermediate in pharaonis Phoborhodopsin"Biophys.J.. 83. 3482-3489 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Y.Furutani, H.Kandori: "Internal Water Molecules of Archaeal Rhodopsins"Mol.Menbr.Biol.. 19. 257-265 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 神取秀樹: "バクテリオロドプシンのプロトンポンプ機構"酵素工学ニュース. 47. 17-22 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 神取秀樹, 加茂直樹: "フォボロドプシンの結晶構造と波長制御・光情報伝達機構"蛋白質核酸酵素. 47. 620-625 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 神取秀樹: "光駆動プロトンポンプの方向性を決める反応"化学. 57. 58-59 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] T.Tanimoto et al.: "Structural Changes of Water in the Schiff Base Region of Bacteriorhodopsin : Proposal of a Hydration Switch Model"Biochemistry. 42. 2300-2306 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] M.Iwamoto et al.: "Proton Transfer Reactions in the F86D and F86E Mutants of Pharaonis Phoborhodopsin (Sensory Rhodopsin II)"Biochemistry. 42. 2790-2796 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 神取秀樹: "視覚の初期過程における光化学反応"レーザー研究. (印刷中). (2003)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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