Mechanism of meiosis initiation in spermatogonia
| Project/Area Number |
14380347
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | Kumamoto University |
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Principal Investigator |
ABE Shin-ichi Kumamoto Univ., Graduate School of Science and Technology, Professor, 理学部, 教授 (90109637)
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| Project Period (FY) |
2002 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥12,400,000 (Direct Cost: ¥12,400,000)
Fiscal Year 2005: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2004: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2003: ¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥4,600,000 (Direct Cost: ¥4,600,000)
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| Keywords | testis / meiosis initiation / spermatogonia / Sertoli cell / neuregulin / FSH / イモリ精巣 / マイクロアレイ / Neuregulin / BMP4 / 変異型BMPR IA / トランスジェニックマウス / 精子形成 / アポトーシス / stem cell factor / c-kit |
|---|
| Research Abstract |
The mechanism underlying meiosis initiation has yet to be clarified. We have shown that there is a checkpoint of meiosis initiation in the 7^<th> generation of spermatogonia in newt testis and presented a hypothesis that meiosis initiation is regulated by the concentration ratio of FSH and prolactin. It was thought that meiosis initiation requires Sertoli cells, upon stimulation by FSH, produce some factor(s) that circumvent apoptosis and act on spermatogonia.
Hence we screened cDNA microarray derived from newt testis and identified neuregulin (NRG) as one of the FSH-upregulated clones. It was revealed that Ig-type NRG is expressed in Sertoli cells, while CRD-type mRNA is expressed in both Sertoli and germ cells. FSH stimulates the expression of Ig-type mRNA highly, but not that of CRD-type. When recombinant protein of the EGF-like domain which is a functional domain of NRG is added to organ culture of the testis, it stimulated the spermatogonial proliferation.
Then we found that NRG mRNA is expressed in mouse testis; NRG1 mRNA level expressed in Sertoli cells decreases as development proceeds, while NRG 3 mRNA level expressed both in Sertoli and germ cells increases. Recombinant proteins of NRG1 and 3 were added to organ culture of testis from 6dpp when only A-type spermatogonia are present, resulting in promotion of spermatogonial proliferation and expression of SPO11 mRNA that is specific to primary spermatocytes. FSH stimulated the expression of NRGs mRNA. These results indicate that NRGs play a pivotal role in meiosis initiation as important factors to act on spermatogonia.
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Report
(5 results)
Research Products
(48 results)
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[Journal Article] Assessment of estrogenic chemicals using an estrogen receptor α (ER α )- and ER β -mediated reporter gene assay in fish.2006
Author(s)
Kitano, T., Koyanagi, T., Adachi, Sakimura, N., Takamune, K., Abe, S.-I.
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Journal Title
Marine Biology (in press)
Description
「研究成果報告書概要(和文)」より
Related Report
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[Journal Article] Assessment of estrogenic chemicals using an estrogen receptor α (ERα)- and ER β -mediated reporter gene assay in fish.2006
Author(s)
Kitano, T., Koyanagi, T., Adachi, R., Sakimura, N., Takamune, K., Abe, S.-I.
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Journal Title
Marine Biology (in press)
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Assessment of estrogenic chemicals using an estrogen receptor α (ERα)- and ERβ-mediated reporter gene assay in fish.2006
Author(s)
Kitano, T., Koyanagi, T., Adachi, R., Sakimura, N., Takamune, K., Abe, S.-I.
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Journal Title
Marine Biology (In press)
Related Report
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[Journal Article] Organochlorine contaminants in human adipose tissues from China : mass balance approach for estimating historical exposure to DDTs by Chinese.2005
Author(s)
Nakata, H., Nasu, T., Abe, S., Kitano, T., Fan, Q., Li W., Ding, X.
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Journal Title
Environmental Science and Technology 39
Pages: 4714-4720
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Grganochlorine contaminants in human adipose tissues from China : mass balance approach for estimating historical exposure to DDTs by Chinese.2005
Author(s)
Nakata, H., Nasu, T., Abe, S., Kitano, T., Fan, Q., Li W., Ding, X.
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Journal Title
Environ.Sci.Technol. 39
Pages: 4714-4720
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Concentrations and compositions of organochlorine contaminants in sediments, soils, crustaceans, fishes and birds collected from lake Tai, Hangzhou bay and Shanghai city region, China.2004
Author(s)
Nakata H., Hirakawa Y., Kawazoe M., Nakabo T., Arizono K., Abe S., Kitano T., Shimada H., Watanabe I., Li W., Ding X.
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Journal Title
Environmental Pollution 133
Pages: 415-429
Description
「研究成果報告書概要(和文)」より
Related Report
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[Journal Article] Concentrations and compositions of organochlorine contaminants in sediments, soils, crustaceans, fishes and birds collected from lake Tai, Hangzhou bay and Shanghai city region, China.2004
Author(s)
Nakata.H., Hirakawa Y., Kawazoe M., Nakabo T., Arizona K., Abe S.,.Kitano T., Shimada H., Watanabe I., Li W., Ding X.
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Journal Title
Environ.Poll. 133
Pages: 415-429
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Expression of Tom34 splicing isoforms in mouse testis and knockout of Tom34 in mice.,2003
Author(s)
Terada, K., Ueno, S., Yomogida, K., Imai, T., Kiyonari, H., Takeda, N., Yano M., Abe, S., Aizawa, S., Mori,M
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Journal Title
J. Biochem 133
Pages: 625-631
NAID
Description
「研究成果報告書概要(和文)」より
Related Report
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[Journal Article] Abnormal spermatogenesis at low temperatures in the Japanese red-bellied newt, Cynops pyrrhogaster : Possible biological significance of the cessation of spermatocytogenesis2003
Author(s)
Yazawa, T., Nakayama, Y., Fujimoto, K., Matsuda, M., Abe, K., Kitano, T., Abe, S, -I, Yamamoto, T.
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Journal Title
Mol Reprod Dev. 66
Pages: 60-66
Description
「研究成果報告書概要(和文)」より
Related Report
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[Journal Article] Expression of Tom34 splicing isoforms in mouse testis and knockout of Tom34 in mice.2003
Author(s)
Terada, K., Ueno, S., Yomogida, K., Imai, T., Kiyonari, H., Takeda, N., Yano, M., Abe, S., Aizawa, S., Mori, M.
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Journal Title
J.Biochem. 133
Pages: 625-631
NAID
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Organochlorine Pesticides and Polychlorinated Biphenyl Residues in Foodstuffs and Human Tissues from China : Status of Contamination, Historical Trend, and Human Dietary Exposure.2002
Author(s)
Nakata, H., Kawazoe, M., Arizono, K., Abe, S., Kitano, T., Shimada, H., Ding, W.Li, X
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Journal Title
Arch. Environ. Contain. Toxicol 43
Pages: 473-480
Description
「研究成果報告書概要(和文)」より
Related Report
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[Journal Article] Organochlorine Pesticides and Polychlorinated Biphenyl Residues in Foodstuffs and Human Tissues from China : Status of Contamination, Historical Trend, and Human Dietary Exposure. Arch. Environ.2002
Author(s)
Nakata, H., Kawazoe, M., Arizona, K., Abe, S., Kitano, T., Shimada, H., Ding, W.Li, X.
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Journal Title
Contam.Toxicol. 43
Pages: 473-480
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Publications] Terada, K., Ueno, S., Yomogida, K., Imai, T., Kiyonari, H., Takeda, N., Yano, M., Abe, S.-I., Aizawa, S., Mori, M.: "Expression of Tom34 splicing isoforms in mouse testis and knockout of Tom34 in mice."J.Biochem.. 133(5). 625-631 (2003)
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[Publications] Yazawa, T., Nakayama Y., Fujimoto K., Matsuda M., Abe, K., Kitano, T., Abe, S.-I., Yamamoto, T.: "Abnormal spermatogenesis at low temperatures in the Japanese red-bellied newt, Cynops pyrrhogaster : Possible biological significance of the cessation of spermatocytogenesis"Mol.Reprod.Dev.. 66(1). 60-66 (2003)
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[Publications] Terada, K., Ueno, S., Yomogida, K., Imai, T., Kiyonari, H., Takeda, N., Yano, M., Abe, S.-I., Aizawa, S., Mori, M.: "Expression of Tom34 splicing isoforms in mouse testis and knockout of Tom34 in mice"Journal of Biochemistry. (In press). (2003)
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[Publications] Nakata, H., Kawazoe, M., Arizono, K., Abe, S.-I., Kitano, T., Shimada, H., Ding, W., Li, X.: "Organochlorine Pesticides and Polychlorinated Biphenyl Residues in Foodstuffs and Human Tissues from China: Status of Contamination, Historical Trend, and Human Dietary Exposure"Arch.Environ.Contam.Toxicol.. 43. 473-480 (2002)
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