| Project/Area Number |
14540386
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
物理学一般
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| Research Institution | RIKEN (The Institute of Physical and Chemical Research) |
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Principal Investigator |
HARADA Yoshihisa RIKEN (The Institute of Physical and Chemical Research), QUANTUM ELECTRONIC MATERIALS RESEARCH TEAM, CONTRACT RESEARCHER, 量子電子材料研究チーム, 連携研究員 (70333317)
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| Co-Investigator(Kenkyū-buntansha) |
KUSUNOKI Masami MEIJI UNIVERSITY, SCHOOL OF SCIENCE & TECHNOLOGY, PROFESSOR, 理工学部, 教授 (30061998)
SUGIURA Miwa OSAKA PREFECTURE UNIVERSITY, COLLEGE OF AGRICULTURE, ASSISTANT, 農学部, 助手 (80312255)
TAKATA Yasutaka SOFT X-RAY SPECTROSCOPY LABORATORY, SENIOR RESEARCHER, 放射光物性研究室, 先任研究員 (90261122)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 2003: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2002: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | SOFT X-RAY EMISSION / SOFT X-RAY ABSORPTION / LIQUID CELL / MANGANESE CLUSTER / 金属蛋白質 |
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| Research Abstract |
The purpose of this study was to perform Mn 2p soft x-ray absorption/emission measurement of the oxygen evolving complex, manganese cluster, in photosystem II. In order to apply soft x-ray absorption/emission measurement which is limited to a solid system or gas system, to a system in solution, we have improved the following three points:
1.Development of the soft X-ray emission spectrometer --balancing high energy resolution and high detection efficiency--
It has become possible to measure the soft x-ray emission from a monolayer adsorbed on a substrate in only 30 minites, with the energy resolution of 1000(E/ΔE).
2.Development of a solution cell with Si_3N_4 membrane to hold a solution in an ultra high vacuum
We have succeeded to maintain about 2μl solution sample between two Si plates with Si3N4 thin films of 200nm thickness using a sealant compatible for ultra high vacuum.
We used this cell to measure Fe 2p soft x-ray absorption and emission measurement of a stable and dense (-10mM) myoglobin.
3.Development of a sample scanning system to reduce radiation damage
We have developed a sample scanning system using the solution cell. However, an opening area toward soft x-rays required for control of a sample position and the opening area restricted for a vacuum-proof did not suit well, so we have given up the scanning of the sample position during measurements. Instead, we have developed a bulk sensitive spectrometer, a high energy photoemission spectrometer, to place a solution sample in a low vacuum.
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