Functional analysis of plastid transcription factors by chloroplast transformation.
| Project/Area Number |
14540598
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物生理
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| Research Institution | Kyoto Prefectural University |
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Principal Investigator |
SHIINA Takashi Kyoto Prefectural University, aculty of Human environment, Professor (10206039)
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| Co-Investigator(Kenkyū-buntansha) |
YAMASHITA Hirofumi Kyoto Prefectural University, Assistant Professor (50264750)
TSUNOYAMA Yiichi Kyoto University, RI research center, Assistant Professor (90314260)
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| Project Period (FY) |
2002 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2004: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2003: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2002: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | PEP / NEP / transcription / sigma factor / promoter / RNA polvmeraze / chloroplast / Arabidopsis / SIG1 / SIG5 / SIBI / 葉緑体形質転換 / 大量発現 / タバコ / RNAポリメラーゼ / 光 / 転写因子 / 転写制御 / プロモータ |
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| Research Abstract |
Plastids are known to have originated from prokaryotic photosynthetic cyanobacteria. Consequently plastids are semi-autonomous and contain their own genetic system. In higher plants, plastids contain a small prokaryotic-type genome which encodes _120 genes. Higher plant plastids contain two types of RNA polymerases, eubacterial-type PEP (plastid-encoded plastid RNA polymerase) and phage-type NEP (nuclearencoded plastid RNA polymerase). In order to recognize promoters, PEP requires an exchangeable subunit, sigma factor. In bacteria, a variety of sigma factors specifically recognize different promoter sequences. Higher plants also encode multiple sigma factor genes. Chloroplast sigma factors are key regulators of the chloroplast gene expression and chloroplast differentiation. This research revealed the roles of plastid sigma factors in chloroplast differentiation and environmental responses. Firstly, we have revealed that SIG6 is an early general sigma factor in plastids that plays a key role in chloroplast development at an early stage of seedling development. Furthermore, we demonstrated that SIG5 acts as a special sigma factor in chloroplasts that selectively recognizes the psbD LRP in response to various environmental stresses.
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Report
(4 results)
Research Products
(66 results)
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[Journal Article] A nuclear-encoded sigma factor, Arabidopsis SIG6, recognizes sigma-70 type chloroplast promoters and regulates early chloroplast development in cotyledons.2005
Author(s)
Ishizaki,Y., Tsunoyama,Y., Hatano,K, Ando,K, Kato,K, Shinmyo,A., Kobori,M., Takeba,G., Nakahira,Y. Shiina,T.
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Journal Title
Plant J. 42
Pages: 133-144
Description
「研究成果報告書概要(和文)」より
Related Report
Peer Reviewed
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[Journal Article] A nuclear-encoded sigma factor, Arabidopsis SIG6, recognizes sigma-70 type chloroplast promoters and regulates early chloroplast development in cotyledons2005
Author(s)
Ishizaki, Y., Tsunoyama, Y., Hatano, K., Ando, K., Kato, K., Shinmyo, A., Kobori, M., Takeba, G., Nakahira, Y., Shiina, T.
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Journal Title
Plant J. 42
Pages: 133-144
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] A nuclear-encoded sigma factor, Arabidopsis SIG6, recognized sigma-70 type chloroplast promoters and regulates early chloroplast development in cotyledo2005
Author(s)
Ishizaki, Y., Tsunoyama, Y., Hatano, K., Ando, K., Kato, K., Shinmyo, A., Nakahira, Y., Shiina, T.
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Journal Title
Related Report
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[Journal Article] A nuclear-encoded sigma factor, AtSig6, plays a key role in early chloroplast development in cotyledons.2005
Author(s)
Ishizaki, Y., Tsunoyama, Y., Hatano, K., Ando, K., Kato, K., Shinmyo, A., Nakahira, Y., Shiina, T.
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Journal Title
Photosynthesis : Fundamental Aspects to Global Perspectives (in press)
Related Report
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[Journal Article] Characterization of a plastid sigma factor AtSIG5 responsible for psbD light-responsive transcription.2005
Author(s)
Nozoe, M., Ishizaki, Y., Tsunoyama, Y., Tsubokura, Y., Kato, K., Shinnmyo, A., Nakahira, Y., Shiina, T.
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Journal Title
Photosynthesis : Fundamental Aspects to Global Perspectives (in press)
Related Report
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[Journal Article] Organellar gene transcription and early seedling development are affected in the rpoT ; 2 mutant of Arabidopsis.2004
Author(s)
Baba,K, Schmidt,J., Espinosa-Ruiz,A., Villarejo,A., Skiina,T., Gardestrom,P., Sane,A.P., Bhalerao,P.
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Journal Title
Description
「研究成果報告書概要(和文)」より
Related Report
Peer Reviewed
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[Journal Article] Organellar gene transcription and early seedling development are affected in the rpoT;2 mutant of Arabidopsis2004
Author(s)
Baba, K., Schmidt, J., Espinosa-Ruiz, A., Villarejo, A., Shiina, T., Gardestrom, P., Sane, A.P., Bhalerao, P.
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Journal Title
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] A role of the -35 element in the initiation of transcription at psbA promoter in tobacco plastids.2003
Author(s)
Hayashi,K., Shiina,T., Ishii,N., Iwai,K., Ishizaki,Y., Morikawa.K., Toyoshima,Y.
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Journal Title
Plant Cell Physiol. 44
Pages: 334-341
NAID
Description
「研究成果報告書概要(和文)」より
Related Report
Peer Reviewed
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[Journal Article] A role of the -35 element in the initiation of transcription at psbA promoter in tobacco plastids2003
Author(s)
Hayashi, K., Shiina, T., Ishii, N., Iwai, K., Ishizaki, Y., Morikawa, K., Toyoshima, Y.
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Journal Title
Plant Cell Physiol. 44
Pages: 334-341
NAID
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] Characterization of a plastid sigma factor AtSIG5 responsible for psbD light-responsive transcription2004
Author(s)
Nozoe,M., Ishizaki,Y., Tsunoyama,Y., Tsubokura,Y., Kato,K., Shinnmyo,A., Nakahira,Y., Shiina,T.
Organizer
国際光合成会議
Place of Presentation
モントリオール
Description
「研究成果報告書概要(和文)」より
Related Report
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[Presentation] A nuclear-encoded sigma factor, AtSig6, plays a key role in early chloroplast development in cotyledons.2004
Author(s)
Ishizaki,Y., Tsunoyama,Y., Hatano,K., Ando,K., Kato,K., Shinmyo,A., Nakahira,Y., Shiina,T.
Organizer
国際光合成会議
Place of Presentation
モントリオール
Description
「研究成果報告書概要(和文)」より
Related Report
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[Presentation] Knock-out of the plastid sigma factor SIG6 in Arabidopsis : effects on transcription of plastid-encoded genes2003
Author(s)
Ishizaki, Y., Tsunoyama, Y., Hatano, K., Takeba, G., nakahira, Y., Shiina, T.
Organizer
Plant Biology 2003
Place of Presentation
Hawaii
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] Characterization of a plastid sigma factor AtSIG5 responsible for psbD light-responsive transcription2003
Author(s)
Nozoe, M., Ishizaki, Y., Tsunoyama, Y., Tsubokura, Y., Kato, K., Shinnmyo, A., Nakahira, Y., Shiina, T.
Organizer
ICP meeting 2003 Photosynthesis : Fundamental Aspects to Global Perspectives
Place of Presentation
Canada
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] A nuclear-encoded sigma factor, AtSig6, plays a key role in early chloroplast development in cotyledons2003
Author(s)
Ishizaki, Y., Tsunoyama, Y., Hatano, K., Ando, K., Kato, K., Shinmyo, A., Nakahira, Y., Shiina, T.
Organizer
ICP meeting 2003 Photosynthesis : Fundamental Aspects to Global Perspectives
Place of Presentation
Canada
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Publications] Tsunoyama, Y., Ishizaki, Y., Morikawam K., Kobori, M., Nakahira, Y., Takeba, G., Toyoshima, Y., Shiina T.: "Blue light-induced transcription of plastid-encoded psbD gene is mediated by a nuclear-encoded transcription iitiation factor, AtSig5"Proc.Natl.Acad.Sci.USA. 101. 3304-3309 (2004)
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[Publications] Baba, K., Schmidt, J., Espinosa-Ruis, A., Villarejo, A., Shiina, T., Gardestrom, P., Sane, A.P., Bhalerao, R.P.: "The dual targeted organells RNA polymerase RpoT ; 2 of Arabidopsis plays a significant role in root growth and early development of plants"Plant J.. (印刷中). (2004)
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