| Project/Area Number |
14550222
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dynamics/Control
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| Research Institution | Osaka Prefecture University |
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Principal Investigator |
HIRAI Yoshihiko Osaka Prefecture University, Graduate School of engineering, Associate professor, 工学研究科, 助教授 (50285300)
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| Co-Investigator(Kenkyū-buntansha) |
KAWATA Hiroaki Osaka Prefecture University, Graduate school of engineering, Associate professor, 工学研究科, 助教授 (90186099)
KIKUTA Hisao Osaka Prefecture University, Graduate school of engineering, Associate professor, 工学研究科, 助教授 (10214743)
TANAKA Yoshio Osaka Prefecture University, Honorary professor, 名誉教授 (60081246)
HAGA Hisashi Hokkaido University, Graduate school of science, Associate professor, 大学院・理学研究科, 助教授 (00292045)
KAWABATA Kazuhshige Hokkaido University, Graduate school of science, Professor, 大学院・理学研究科, 教授 (20261274)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2003: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2002: ¥2,700,000 (Direct Cost: ¥2,700,000)
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| Keywords | visco-eleastic / cell / living material / atomic force microscopy / カンチレバー / フォースモジュレーション法 / 表面剛性 / 表面粘性 |
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| Research Abstract |
Understanding cellular migration as an integrated mechanical system requires an experimental investigations on mechanical properties of living cells. Force modulation mode with SPM is proposed as a useful method fur measuring stiffness of living cells with high temporal and spatial resolution. However, in liquid environment, cellular stiffness obtained by force modulation mode is often incredible.
To clear this problem, numerical analysis of the 1-dimensional dynamic equation of a-micro cantilever, which concern about the visco-elastic property of the sample and viscosity of the liquid, is carried out to evaluate the relations between phase shift and amplitude modulations when the cantilever contacts with the living cells in a liquid. Also, the vibration analysis is performed when the cantilever periodically contact and not contact to the sample for various visco-elastic samples. Based on those results, the quantitative relations between vibration property of the cantilever and visco-elastic property of the sample are derived. Using these results, analysis system of the visco-elastic property for living cells is newly developed and the mechanical property of the living cells are demonstrated.
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