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The development of an optimal mushroom farming system for the edible mushroom, using the bioelectric potential of the mushrooms as an index, with a LED as a source of illumination

Research Project

Project/Area Number 14550429
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Measurement engineering
Research InstitutionKanazawa Institute of Technology

Principal Investigator

HIRAMA Junji  Kanazawa Institute of Technology, Division of Electrical Engineering, Professor, 工学部, 教授 (40181185)

Co-Investigator(Kenkyū-buntansha) MIYAMOTO Toshio  Kanazawa Institute of Technology, Division of Electrical Engineering, 工学部, 教授 (20229892)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2003: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2002: ¥2,400,000 (Direct Cost: ¥2,400,000)
KeywordsMushroom / Optimum Growth Condition / Bio-Electric Potential / Light Stimuli / Wavelength Dependence / Genetic Analysis / Morphgenetic Property / MRI System / 至適生育条件
Research Abstract

The scientific study of the optimal growth conditions for mushroom farming, such as culture medium, temperature, humidity, illumination, etc, has been making slow progress. By observing the bioelectric-potential of mushrooms, which is considered to be closely related to the growth, the relationship between light stimulus conditions (wavelength, brightness, and illumination methods) and the morphogenetic properties has been investigated. A new type of light source was developed out of studies of the optimum farming conditions from an engineering standpoint. Photographs were taken non-destructively of the growth of fungal threads by MRI. Furthermore, with regard to the effect of the light quality on the morphogenesis, genetic investigations were made into the expression of DNA by an advanced study of bio-molecules.
Here are the outlines of this study :
(1)Systematic changes in light stimulus factor :
An improvement was made in the accurate auto-measurement system for recording bio-electric … More potentials stimulated by illumination in a purpose-built growth chamber.
(2)Measurement of very weak spontaneous magnetic field :
The very weak spontaneous magnetic field generated by the mushrooms were measured using a SQUID as a non-destructive and non-invasive measuring technique. The effectiveness of the use of the measured results in the study of ripeness of the fungal threads in the culture medium or growth levels of the pileus was evaluated.
(3)Development of a light source at the mushroom farming site :
Using the improved purpose-built simple growth chamber, comparative experiments were conducted on morphogenetics with various light stimuli. Bio-electric potential sensing was also undertaken at the same time in order to make health checks on the mushrooms. The effectiveness of the use of mushrooms as a bio-sensor as an environmental monitor was also studied. Currently, a comparison study of morphogenesis under various illumination regimes has been conducted, including continuous illumination, fluctuated illumination, pulsed illumination, etc. However, no clear conclusions have yet been arrived at.
(4)Cross sectional photographs taken using MRI :
The photographs of the mushroom growth process from fungus through to the formation of primordium have been taken, non-destructively and non-invasively, every two weeks. The results have led to a perspective study of the mushroom ripeness.
(5)Genetic analysis :
With regard to the influence of the characteristics of illumination on the morphogenesis, the messenger RNA(mRNA) was detected using the DD method and compared for analysis. Less

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] 松田真人, 平間淳司, 他3名: "マイタケにおける光照射時の自発性極微弱磁場信号計測"日本植物工場学会誌. 16巻・1号. 20-24 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 柳橋秀幸, 平間淳司, 他5名: "光刺激による茸(舞茸)の生体電位応答(第3報)-周囲温度と生体電位との関係"日本植物工場学会誌. 15巻・2号. 94-97 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Junji Hirama, Masato Matsuda, Hideyuki Yanagibashi: "Relationship between the bio-electric potential and the morphogenetic properties of Grifola Frondosa when subjected to different light stimuli"First Japan-Thailand Joint Symposium on Advanced Technologies in Agricultual Production and Post-Production. (印刷準備中). (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 古澤紀光, 平間淳司, 他4名: "光刺激に応答した舞茸の子実体発達過程における発現遺伝子の検出"農業環境工学関連学会2003年合同大会 講演論文集. 153 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 平間淳司, 柳橋秀幸, 他3名: "光刺激による舞茸の子実体の生体電位応答と形態形成との関係"電気学会論文誌. 122巻・9号. 1679-1685 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 原田健一, 平間淳司, 他3名: "MRI装置を用いた舞茸培地内部の菌糸の撮影-植菌直後から原基形成に至るまで"日本植物工場学会中部支部創立10周年記念大会 梗概集. 30-31 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Masato Matsuda, Junji Hirama: "Measurement of Very Weak Spontaneous Magnetic Fields Associated with Photo Irradiated Grifola frondosa"Journal of Society of High Technology in Agriculture. 16(1). 20-23 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hideyuki Yanagibashi, Junji Hirama: "Bio-Electric Potential under Light Stimuli of Grifola frondosa.(Part 3) -Relatinship Between the Environmental Temperature and the Bio-Electric Potential-"Journal of Society of High Technology in Agriculture. 15(2). 94-97 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Junji Hirama: "Relatinship between the Bio-electric Potential and the Morphogenetic Property of Grifola frondosa when Subjected to Different Light Stimuli"First Japan-Thailand Joint Symposium on Advanced Technologies in Agricultual Production and Post-Production. (Now Printing). (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Junji Hirama: "Relationship between the bio-electric potential and the morphogenetic property of Grifola Frondosa under different light stimuli"Trans IEE of Japan. Vol.122-C, No.9. 1679-1685 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 松田真人, 平間淳司, 他5名: "マイタケにおける光照射時の自発性極微弱磁場信号計測"日本植物工場学会誌. 16巻1号. 20-23 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 柳橋秀幸, 平間淳司. 他5名: "光刺激による茸(舞茸)の生体電位反応(第3報)-周囲温度と生体電位との関係-"日本植物工場学会誌. 15巻2号. 94-97 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 原田健一, 平間淳可, 他6名: "MRI装置を用いた培地内部の撮影(2)-植菌から子実体形成に至るまで-"農業環境工学関連学会2003年合同大会. 154 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Junji Hirama, Masato Matsuda, Hideyuki Yanagibashi: "Relationship between the bio-electric potential and the morphogenetic properties of Grifola Frondosa when subjected to different light stimuli"First Japan-Thailand Joint Symposium on Advanced Technologies in Agricultural Production and Post-Production. (印刷準備中). (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 古澤紀光, 平間淳司, 他4名: "光刺激に応答した舞茸の子実体発達過程における発現遺伝子の検出"農業環境工学関連学会2003年合同大会. 153 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 坂本香織, 平間淳司, 他6名: "なめこ子実体の光形態形成初期過程における発現遺伝子の解析"農業環境工学関連学会2003年合同大会. 361 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 柳橋秀幸, 平間淳司, 他5名: "光刺激による茸(舞茸)の生体電位反応(第3報)-周囲温度と生体電位との関係-"日本植物工場学会誌. 15巻・2号(掲載決定). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 平間淳司, 柳橋秀幸, 他3名: "光刺激による舞茸の子実体の生体電位応答と形態形成との関係"電気学会論文誌. 122巻・9号. 1679-1685 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 平間淳司: "舞茸培地の培養段階が菌糸の発達へ与える影響-MRI装置を用いた培地内部の画像-"ファイトテクノロジー研究会. (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 原田健一, 平間淳司, 他3名: "MRI装置を用いた舞茸培地内部の菌糸の撮影-植菌直後から原基形成に至るまで-"日本植物工場学会中部支部創立10周年記念大会梗概集. 30-31 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 神野克典, 平間淳司, 他5名: "光刺激に応答した子実体形成初期段階における発現遺伝子の解析"農業環境工学関連4学会2002年合同大会. 161 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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