• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Molecular recognition process in the ultrathin-film surface observed by surface plasmon spectroscopy (SPR)

Research Project

Project/Area Number 14550854
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 高分子構造・物性(含繊維)
Research InstitutionNATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY

Principal Investigator

TAWA Keiko  National Institute of Advanced Industrial Science and Technology, Research Institute for Cell Engineering, senior researcher, セルエンジニアリング研究部門, 主任研究員 (80344109)

Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2004: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2003: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2002: ¥2,600,000 (Direct Cost: ¥2,600,000)
KeywordsRotaxane ultrathin-film / Poly(ethylene glycol) / Optical control / Azobenzene / Cyclodextrin / Surface Plasmon Spectroscopy / Self-assembled monolayer / 表面プラズモン励起増強蛍光分光法 / 分子認識 / ロタキサン / 超薄膜
Research Abstract

It has been known that poly (ethylene glycol)(PEG) can form a polyrotaxane with α-cyclodextrin(α-CD) due to the molecular recognition. In this study, a self-assembled-monolayer(SAM) composed of mixture thiols, MeAzoPEGn (n=6,10,16) and diluent thiol (11-undecanol thiol : HSC11OH or dodecane thiol : HSC12), was prepared, and the formation process of polyrotaxane-ultrathin film with α-CD and its photo-controllability were investigated by the surface plasmon resonance spectroscopy(SPR).
The novel compounds, MeAzoPEGn, synthesized by ourselves consist of 4-methyl azobenzene moiety, PEG (unit number:6,10,16), and mercapt group. The increase in the film thickness of 2.0±0.2 nm was observed at the SAM of MeAzoPEG6 after addition of α-CD solution. α-CD rings were found to form polyrotaxane-film selectively with MeAzoPEG6 chain regardless of the kind of the diluent thiols. 4-methyl azobenzene moiety substituted at the end of MeAzoPEG6 is considered to be a trigger molecule to capture α-CD rings and then, CD rings can penetrate through a PEG chain. As the result, a PEG chain was extended and a new rotaxane layer was observed. In the case of the SAM of MeAzoPEG10 and MeAzoPEG16,the increase in the film thickness by adding α-CD was larger. It is considered to be due to that more CD molecules are possible to form a thicker polyrotaxane-film for the longer PEG chain.
Furthermore, the formation of polyrotaxane-film was investigated under UV irradiation. UV irradiation induces the isomerization of azobenzene moiety and the increase in the film thickness after adding α-CD was suppressed by 1.4 nm. The complete control was impossible but the partial control was possible for the formation of the polyrotaxane-film by UV light irradiation.

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (21 results)

All 2005 2004 2003 2002 Other

All Journal Article (14 results) Book (1 results) Patent(Industrial Property Rights) (2 results) Publications (4 results)

  • [Journal Article] Dependence of the Kinetics of DNA-DNA Hybridization Studied by Surface Plasmon Fluorescence Spectroscopy2005

    • Author(s)
      Keiko Tawa, Danfeng Yao, Wolfgahg Knoll
    • Journal Title

      Biosensors and Bioelectronics (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A novel azobenzene-containing polyamic acid with excellent Langmuir-Blodgett-Kuhn film formation behavior suitable for all optical-switching2005

    • Author(s)
      Y.Zong, K.Tawa, B.Menges, J.Ruhe, W.Knoll
    • Journal Title

      Langmuir (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Polyrotaxane-Film Formation Observed by Surface Plasmon Spectroscopy2005

    • Author(s)
      K.Tawa, N.Kuboyama, M.Tanaka
    • Journal Title

      MRS-J proceedings 30(in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Dependence of the Kinetics of DNA-DNA Hybridization Studied by Surface Plasmon Fluorescence Spectroscopy2005

    • Author(s)
      K.Tawa, D.Yao, W.Knoll
    • Journal Title

      Biosensors and Bioelectronics in press

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Mismatching base-pair dependence of the kinetics of DNA-DNA hybridization studied by surface plasmon fluorescence spectroscopy2004

    • Author(s)
      Keiko Tawa, Wolfgang Knoll
    • Journal Title

      Nucleic Acids Research 32(8)

      Pages: 2372-2377

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Fluorescence Emission Control and Switching of Oxymethylcrowned Spirobenzopyrans by Metal Ion2004

    • Author(s)
      S.A.Ahmed, M.Tanaka, H.Ando, K.Tawa, K.Kimura
    • Journal Title

      Tetrahedron 60

      Pages: 6029-6036

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Mismatching base-pair dependence of the kinetics of DNA-DNA hybridization studied by surface plasmon fluorescence Spectroscopy2004

    • Author(s)
      K.Tawa, W.Knoll
    • Journal Title

      Nucleic Acids Research 32(8)

      Pages: 2372-2377

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Fluorescence Emission Control and Switching of Oxymethylcrowned Spirobenzopirans by Metal Ion2004

    • Author(s)
      A.A.Saleh, M.Tanaka, H.Ando, K.Tawa, K.Kimura
    • Journal Title

      Tetrahedron 60

      Pages: 6029-6036

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Out of Plane Photoreorientation of Azo Dyes in Polymer Thin Films Studied by Surface Plasmon Resonance Spectroscopy2002

    • Author(s)
      Keiko Tawa, Wolfgang Knoll
    • Journal Title

      Macromoolecules 35(18)

      Pages: 7018-7023

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Polarized-Light Induced Orientation of Azo-Dyes Polymer Matrix Studied by Polarized Spectroscopy2002

    • Author(s)
      Keiko Tawa
    • Journal Title

      Kobunshi Ronbunshu 59(8)

      Pages: 499-509

    • NAID

      10009433499

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Out-of-Plane Photoreorientation of Azo-Dyes in Polymer Thin Films Studied by Surface Plasmon Resonance Spectroscopy2002

    • Author(s)
      K.Tawa, W.Knoll
    • Journal Title

      Macromolecules 35(18)

      Pages: 7018-7023

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Polarized-Light Induced Orientation of Azo-Dyes Polymer Matrix Studied by Polarized Spectroscopy2002

    • Author(s)
      K.Tawa
    • Journal Title

      Kobunshi Ronbunshu 59(8)

      Pages: 499-509

    • NAID

      10009433499

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Dependence of the Kinetics of DNA-DNA Hybridization Studied by Surface Plasmon Fluorescence Spectroscopy

    • Author(s)
      K.Tawa, D.Yao, W.Knoll
    • Journal Title

      Biosensors and Bioelectronics (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Polyrotaxane-Film Formation Observed by Surface Plasmon Spectroscopy

    • Author(s)
      K.Tawa, N.Kuboyama, A.Saleh, M.Tanaka
    • Journal Title

      MRS-J proceedings 30(in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] A novel azobenzene-containing polyamic acid with excellent Langmuir-Blodgett-Kuhn film formation behavior suitable for all optical-switching

    • Author(s)
      Y.Zong, K.Tawa, B.Menges, J.Ruhe, W.Knoll
    • Publisher
      Langmuir(in press)
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Patent(Industrial Property Rights)] プラズモン共鳴蛍光を用いた生体分子相互作用検出装置及び検出方法2005

    • Inventor(s)
      田和 圭子, 田口 隆久
    • Industrial Property Rights Holder
      産業技術総合研究所
    • Industrial Property Number
      2005-170808
    • Filing Date
      2005-01-26
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Patent(Industrial Property Rights)] アゾアリールメルカプトアルキルポリエチレングリコール2003

    • Inventor(s)
      田和 圭子, 田中 睦生
    • Industrial Property Rights Holder
      産業技術総合研究所
    • Industrial Property Number
      2003-287629
    • Filing Date
      2003-08-06
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Keiko Tawa: "Mismatching base-pair dependence of the kinetics of DNA-DNA hybridization studied by surface plasmon fluorescence spectroscopy"Nucleic Acids Research. in press. (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 田和圭子: "高分子辞典第3版(可視吸収スペクトルなど一部の項目)"朝倉書店. 2 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Keiko Tawa, Wolfgang Knoll: "Out of Plane Photoreorientation of Azo Dyes in Polymer Thin Films Studied by Surface Plasmon Resonance Spectroscopy"Macromoolecules. 35(18). 7018-7023 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Keiko Tawa: "Polarized-Light Induced Orientation of Azo-Dyes Polymer Matrix Studied by Polarized Spectroscopy"Kobunshi Ronbunshu. 59(8). 499-509 (2002)

    • Related Report
      2002 Annual Research Report

URL: 

Published: 2002-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi