| Project/Area Number |
14560053
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plant nutrition/Soil science
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
NAGAOKA Toshinori Hiroshima University, Graduate School of Biosphere Science, Associate Professor, 大学院・生物圏科学研究科, 助教授 (80237502)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 2003: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2002: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | Cruciferae / Clubroot disease / Plasmodiophora brassicae / Resting snore / Organic matter / Rapeseed meal / Plasmodiophara brassicae / 油粕 |
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| Research Abstract |
1. Soil samples amended with various organic matters were inoculated with resting spores of Plasmodiophora brassicae, and incubated. Rapeseed meal remarkably reduced the number of resting spores in the soil, as compared with other organic matters. Application of rapeseed meal to the field infested with P.brassicae at 5t/ha also decreased the number of resting spores, indicating the effectiveness of rapeseed meal in the field condition
2. Water extracts of rapeseed meal and the soils amended with rapeseed meal did not show any stimulating effects on the germination of resting spores of P.brassicae in germination tests. It was suggested that the reduction of the number of resting spores would not be caused by the germination of resting spores
3. Leafy daikon (Raphanus sativus) and Oats (Avena sariva), which were known decoy plants reducing the resting-spore density of P.brassicae in soil, were cultured in pots filled with the inoculated soil after the amendment with rapeseed meal. The number of resting spores in amended soil decreased more considerably than non-amended soil, suggesting the availability of cultivating the decoy plants in combination with application of rapeseed meal to soil
4. The number of resting spores of P.brassicae in soil decreased in lower soil-moisture contents, and more significantly at 35℃ than at 25℃ in incubation experiments. Autoclaving of soil before inoculation suppressed the reduction of the number of resting spores in soil, suggesting the involvement of soil microorganisms in the reduction of the number of resting spores
5. Phospholipid fatty acid profiles of the soils amended with various organic matters exhibited the differences of microbial community structure among the soils amended with different organic matters. Further investigations are required into soil microbial community involving the reduction of the number of resting spores
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