| Project/Area Number |
14560092
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
食品科学・栄養科学
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| Research Institution | Tokyo University Agriculture And Technology |
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Principal Investigator |
HATTORI Makoto Tokyo University of Agriculture and Technology, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (40221501)
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| Co-Investigator(Kenkyū-buntansha) |
KONISHI Yoshiko National Institute of Health Sciences, Division of Microbiology, Chief of the fourth section, 衛星微生物部, 室長(研究職) (10195761)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2003: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2002: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | intestinal infection / prevention / glycopeptide / glycomacropeptide / ovomucin |
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| Research Abstract |
Preventive effects of glycomacropeptide (GMP) and ovomucin glycopeptide (OGP) against intestinal infection were investigated and the conjugates of GMP with xylooligosaccharide (XOS) and carboxymethyldextran (CMD) were prepared by the Maillard reaction to enhance the effect of GMP. Binding ability of GMP to intestinal pathogenic bacteria was evaluated by ELISA with biotinylated bacteria. GMP showed binding ability to Salmonella enteritidis (S.enteritidis) and enterohaemorrhic Escherichia coli O157:H7 (EHEC O157). OGP showed binding ability to EHEC O157. These binding abilities markedly decreased by sialydase treatment and were completely eliminated by penodate oxidation. These results indicated that carbohydrate moieties such as sialic acid in GMP and OGP are involved in binding to these bacteria. The effect of GMP on the adhesion and invasion of pathogenic bacteria to Caco-2 cells was also investigated. GMP was not potent inhibitor to adhesion and invasion of Salmonella infection. However, GMP-XOS and GMP-CMD significantly suppressed IL-8 production which was the index of infection. Our results indicate that GMP and OGP are promising as the agent to prevent intestinal infection.
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